Anti-biotin Antibody, clone F1 | PA007522.h1
$300.00 – $1,500.00
Recombinant anti-biotin antibodies and mutants good for in vitro and in vivo studies. The variable regions were not humanized in the human antibody versions.
- Details & Specifications
- References
| Catalog No. | PA007522.h1 |
|---|---|
| Product Name | In vivo Grade Recombinant Anti-biotin Antibody, Human IgG1 Kappa |
| Supplier Name | Syd Labs, Inc. |
| Brand Name | Syd Labs |
| Synonyms | Biotin; Vitamin H; Vitamin B7 |
| Clone | F1. |
| Isotype | Human IgG1 kappa. |
| Specificity/Sensitivity | Biotin. |
| Applications | ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research. |
| Form Of Antibody | 0.2 uM filtered solution of 1x PBS. |
| Endotoxin | < 1 EU per 1 mg of the protein by the LAL method. |
| Purity | >95% by SDS-PAGE under reducing conditions. |
| Shipping | The In vivo Grade Recombinant Anti-biotin Antibody, Human IgG1 Kappa is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied. |
| Note | Recombinant anti-biotin antibodies and mutants good for in vitro and in vivo studies. The variable regions were not humanized in the human antibody versions. |
| Order Offline | Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card. |
Description
PA007522.h1: Recombinant Anti-biotin Antibody, Human IgG1 Kappa
Anti-biotin antibody is an antibody that specifically binds to biotin, a small vitamin (vitamin B7) involved in various metabolic processes. These antibodies are typically raised in animals (e.g., rabbits, goats, or mice) by immunizing them with biotin conjugated to a carrier protein, as biotin alone is too small to elicit a strong immune response.
Anti-biotin antibodies are widely used in research and biotechnology, particularly in techniques like ELISA, Western blotting, or immunohistochemistry, where biotin’s strong affinity for proteins like streptavidin or avidin is exploited. For example, a biotinylated molecule (e.g., a protein or nucleic acid tagged with biotin) can be detected or captured using an anti-biotin antibody, which may be conjugated to a reporter molecule (like an enzyme or fluorophore) for visualization or quantification.
In essence, anti-biotin antibodies serve as tools to detect or manipulate biotin-tagged molecules in various experimental setups.
The in vivo grade recombinant anti-biotin antibody (human IgG1 kappa) of Syd Labs was produced in mammalian cells.
Syd Labs provides the following in vivo grade recombinant anti-Biotin/DNP/Fluorescein antibodies:
In Vivo Grade Recombinant Anti-Biotin Monoclonal Antibodies (Clone F1)
In Vivo Grade Recombinant Anti-DNP Monoclonal Antibodies (Clone DNP.m1)
In Vivo Grade Recombinant Anti-Fluorescein (FITC) Monoclonal Antibodies (Clone 4-4-20 (enhanced))
Recombinant Anti-biotin Antibody(Clone f1) from: In vivo Grade Recombinant Anti-biotin Antibody, Human IgG1 Kappa: PA007522.h1 Syd Labs
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