Anti-biotin Antibody, Mouse IgG2a
$300.00 – $1,500.00
Recombinant anti-biotin antibodies and mutants good for in vitro and in vivo studies. The variable regions were not humanized in the human antibody versions.
- Details & Specifications
- References
| Catalog No. | PA007522.m2a |
|---|---|
| Product Name | In vivo Grade Recombinant Anti-biotin Antibody, Mouse IgG2a Kappa |
| Supplier Name | Syd Labs, Inc. |
| Brand Name | Syd Labs |
| Synonyms | Biotin; Vitamin H; Vitamin B7 |
| Clone | F1. |
| Isotype | Mouse IgG2a Kappa |
| Specificity/Sensitivity | Biotin. |
| Applications | ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research. |
| Form Of Antibody | 0.2 uM filtered solution of 1x PBS. |
| Endotoxin | < 1 EU per 1 mg of the protein by the LAL method. |
| Purity | >95% by SDS-PAGE under reducing conditions. |
| Shipping | The in vivo grade recombinant anti-biotin antibodies (clone of F1) is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 1 month from date of receipt, 2 to 8°C as supplied. 12 months from date of receipt, -20 to -70°C as supplied. |
| Note | Recombinant anti-biotin antibodies and mutants good for in vitro and in vivo studies. The variable regions were not humanized in the human antibody versions. |
| Order Offline | Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card. |
Description
PA007522.m2a: In vivo Grade Recombinant Anti-biotin Antibody, Mouse IgG2a Kappa
The in vivo grade recombinant anti-biotin antibody, Mouse IgG2a Kappa was produced in mammalian cells.
Background of Recombinant Anti-biotin Antibody
Recombinant mouse anti-biotin antibodies recognize biotin (vitamin B7) attached to proteins, peptides, nucleic acids, or solid matrices, and can be offered in fluorophore and enzyme-conjugated versions.
The anti-biotin monoclonal antibody can be used as a second-step reagent in various applications for detecting biotinylated targets. The biotin-streptavidin system is widely used in immunoassay signal amplification techniques because streptavidin binds biotin with extremely high affinity.
Because biotin is naturally present in a large variety of biological samples, it may affect the results of biotin-based assays, such as reduced sensitivity, false negatives or false positives. Streptavidin/biotin blocking kit, avidin/biotin blocking system, or biotin blockers are used to block signals from endogenous avidin, biotin and biotin-binding proteins in tissues when used with biotin-based IHC detection.
Please contact us to ask for a quote for the engineered anti-biotin antibody mutants of various isotypes, and mutants with Avi-, His-, and DYKDDDDK-tags, and biotinylated antibodies and mutants. A variety of conjugates (such as dyes and fluorophores) with the anti-biotin antibodies and mutants are available.
Syd Labs provides the following in vivo grade recombinant anti-Biotin/DNP/Fluorescein antibodies:
In Vivo Grade Recombinant Anti-Biotin Monoclonal Antibodies (Clone F1)
In Vivo Grade Recombinant Anti-DNP Monoclonal Antibodies (Clone DNP.m1)
In Vivo Grade Recombinant Anti-Fluorescein (FITC) Monoclonal Antibodies (Clone 4-4-20 (enhanced))
n vivo Grade Recombinant Anti-biotin Antibody, Mouse IgG2a Kappa from: n vivo Grade Recombinant Anti-biotin Antibody, Mouse IgG2a Kappa: PA007522.m2a Syd Labs
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