Anti-Human CD56 (N901) Antibody | PA007278.m2a

$150.00 – $900.00

Recombinant mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.

Details & Specifications
References

Catalog No.	PA007278.m2a
Product Name	In vivo Grade Recombinant Anti-human CD56 Monoclonal Antibody (Clone: N901), Mouse IgG2a Kappa
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	Neural cell adhesion molecule, NCAM, CD56, MSK39, neural cell adhesion molecule 1, NCAM1
Clone	N901
Isotype	Mouse IgG2a kappa
Source/Host	The anti-human CD56 monoclonal antibody (clone: N901) was produced in mammalian cells.
Specificity/Sensitivity	The in vivo grade recombinant mouse monoclonal antibody (clone: N901) specifically binds to the human CD56 protein.
Applications	ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human CD56 protein.
Form Of Antibody	0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin	< 1 EU per 1 mg of the protein by the LAL method.
Purity	>95% by SDS-PAGE under reducing conditions and HPLC.
Shipping	The In vivo Grade Recombinant Anti-human CD56 Monoclonal Antibody (Clone: N901), Mouse IgG1 Kappa is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007278.m2a: Recombinant Anti-human CD56 Antibody (Clone: N901), Mouse IgG2a Kappa, In vivo Grade

Recombinant mouse IgG2a Monoclonal Antibody.

Background of Anti-human CD56 Antibody (Clone: N901)

The recombinant N901 antibody binds to the human CD56 protein, a homophilic binding glycoprotein expressed on the surface of neurons, glia and skeletal muscle. CD56 is a marker of neural lineage commitment. CD56 expression is found in the hematopoietic system, natural killer cells, and other lymphoid cells, including gamma delta (γδ) Τ cells and activated CD8+ T cells, as well as on dendritic cells. CD56 has a role in cell–cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory.

References of Anti-Human CD56 Monoclonal Antibody (Clone: N901)

1. Advanced Immune Cell Profiling by Multiparameter Flow Cytometry in Humanized Patient-Derived Tumor Mice.
Bruss, C., et al. Cancers (Basel). 2022 Apr 28;14(9):2214. doi: 10.3390/cancers14092214. PMID: 35565343
“The N901 antibody (anti-human CD56, PE-conjugated) was used in multiparameter flow cytometry to characterize immune cells in humanized patient-derived tumor mice. Staining was performed on spleen single-cell suspensions and peripheral blood using FACSCanto-II or Navios cytometers, with DuraClone protocols, to assess CD56 expression alongside markers like CD3, CD19, and CD45 for immune profiling…”

2. Advanced Flow Cytometry Assays for Immune Monitoring of CAR-T Cell Applications.
Blache, U., et al. Front Immunol. 2021 May 3;12:658314. doi: 10.3389/fimmu.2021.658314. PMID: 34012442
“The N901 antibody (anti-human CD56) was included in antibody panels (TCR, RE ALB, CAR-T1, CAR-T2, NK1, NK2) for staining EDTA blood samples in CAR-T cell immune monitoring. Flow cytometry using a DxFLEX cytometer analyzed NK cell populations, with 100 µl whole blood stained for 15 minutes to assess CD56 expression in CAR-T therapy contexts…”

3. EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes.
van Dongen, J. J. M., et al. Leukemia. 2012 Sep;26(9):1908-39. doi: 10.1038/leu.2012.120. PMID: 22552007
“The N901 antibody (anti-human CD56) was part of the EuroFlow antibody panels for standardized flow cytometric immunophenotyping of leukocytes. Used with EuroFlow SOPs on FACSCanto-II cytometers and Infinicyt software, it facilitated consistent analysis of CD56 expression in normal, reactive, and malignant leukocytes, supporting WHO-based diagnosis of hematological malignancies…”

4. Detection of the CD56+/CD45- immunophenotype by flow cytometry in neuroendocrine malignancies.
Bryson, G. J., et al. J Clin Pathol. 2002 Jul;55(7):535-7. doi: 10.1136/jcp.55.7.535. PMID: 12101203
“The N901 antibody (anti-human CD56, PE-conjugated) was used in a three-color flow cytometry assay (with anti-CD3-FITC and anti-CD45-PECY5) on a Beckman Coulter XL cytometer to identify the CD56+/CD45- immunophenotype in neuroendocrine malignancies, such as metastatic small cell carcinoma, confirming its utility in lymphoma evaluation…”

5. Aggressive natural killer cell leukemia/lymphoma with N901-positive surface phenotype: evidence for the existence of a third lineage in lymphoid cells.
Imamura, N., et al. Acta Haematol. 1988;80(3):157-64. doi: 10.1159/000205617. PMID: 2461636
“The N901 antibody (anti-human CD56, NKH-1) identified CD56 expression on leukemic cells with high NK activity in aggressive NK cell leukemia/lymphoma. Flow cytometry showed these cells lacked Leu 7 (HNK-1) and T/B-cell lineage markers, confirming a distinct third lymphoid lineage without T beta, T gamma, or immunoglobulin gene rearrangement…”

Anti-human CD56 (N901) from: Anti-human CD56 Monoclonal Antibody (Clone: N901), Mouse IgG2a Kappa: PA007278.m2a Syd Labs