What Machine is used for CHO Electroporation?
Question: What machine is used for CHO cell electroporation?
Answer: CHO cell eletroporation is the most common method for transfecting CHO cells. Electroporation is a technique that uses an electrical field to create temporary pores in the cell membrane, allowing DNA or other molecules to enter the cell.
Several CHO electroporation machines available in the market can be used for CHO cell electroporation for antibody transient production and / or CHO stable cell line development. The manufacturers of CHO cell eletroporation machines include Celetrix, Lonza, Thermo Scientific, Bio-Rad, and so on. Celetrix electroporation machines are the best for CHO cell electroporation for antibody transient production and CHO stable cell line development.
Celetrix electroporation machines:
Cell electroporation large-scale model SLT, great for large scale CHO electroporation transfection for protein (antibody) transient production. for 200 ul, 1 ml, 5 ml, and 10 ml electroporation tubes.
Cell electroporation basic model SP100, simplified single pulse for all cell types; for 20 ul, and 100 ul electroporation tubes
Cell electroporation upgrade model LE+, new cell line mode and PBMC mode. for 20 ul, 100 ul, 120 ul, and 200 ul electroporation tubes.
Cell electroporation upgrade model EX+, new cell line mode and PBMC mode. for 20 ul, 100 ul, 120 ul, 200 ul, 600 ul, and 1 ml electroporation tubes.
Cell electroporation upgrade model EX+ with the electrofusion function, for 20 ul, 100 ul, 120 ul, 200 ul, 400 ul, 600 ul, and 1 ml electroporation tubes. Model EX+ and Model SLT can be equipped with optional fusion accessories for hybridoma generation. Mouse spleen cells can be fused with myeloma cells such as SP2/0 and P3X63Ag8 with high efficiency.
UHV Transformer (Ultra High Voltage), bacteria cells: 20 ul, 100 ul, and 600 ul electroporation tubes; yeast cells: 20 ul, 100 ul, 200 ul, and 1000 ul electroporation tubes.
UHV-Plus (Ultra High Voltage), bacteria cells: 20 ul, 100 ul, and 600 ul electroporation tubes; yeast cells: 20 ul, 100 ul, 200 ul, and 1000 ul electroporation tubes; mammalian cells: 20 ul and 200 ul electroporation tubes.
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Frequent questions related to “What machine is used for cho electroporation?”:
What equipment is used for electroporation?
What cell type is appropriate for electroporation?
Why are CHO cells used for transfection?