What is the difference between CHO chemical transfection and CHO electroporation transfection?
Question: What is the difference between CHO chemical transfection and CHO electroporation transfection?
Answer: Both chemical transfection and electroporation transfection are widely used for introducing foreign nucleic acids into CHO cells. Here are some advantages and disadvantages of each method:
Advantages of CHO chemical transfection: simple and easier to perform; no need of expensive equipment; a wide range of cell types.
Disadvantages of CHO chemical transfection: lower transfection efficiency; toxicity of transfection reagents affecting cell viability and function; DNA uptake limited to a small fraction of cells.
Advantages of CHO electroporation transfection: higher transfection efficiency; minimal toxicity to cells; a wide range of cell types.
Disadvantages of CHO electroporation transfection: expensive equipment; technically challenging; higher percentage of cell damage or death. The advantage of Celetrix electroporation machines for CHO cell transfection is lower percentage of cell damage and death than the machines from other manufacturers.
Celetrix electroporation machines:
Cell electroporation basic model SP100, simplified single pulse for all cell types; for 20 ul, and 100 ul electroporation tubes
Cell electroporation upgrade model LE+, new cell line mode and PBMC mode. for 20 ul, 100 ul, 120 ul, and 200 ul electroporation tubes.
Cell electroporation upgrade model EX+, new cell line mode and PBMC mode. for 20 ul, 100 ul, 120 ul, 200 ul, 600 ul, and 1 ml electroporation tubes.
Cell electroporation large-scale model SLT, great for large scale CHO electroporation transfection for protein (antibody) transient production. for 200 ul, 1 ml, 5 ml, and 10 ml electroporation tubes.
Cell electroporation upgrade model EX+ with the electrofusion function, for 20 ul, 100 ul, 120 ul, 200 ul, 400 ul, 600 ul, and 1 ml electroporation tubes. Model EX+ and Model SLT can be equipped with optional fusion accessories for hybridoma generation. Mouse spleen cells can be fused with myeloma cells such as SP2/0 and P3X63Ag8 with high efficiency.
UHV Transformer (Ultra High Voltage), bacteria cells: 20 ul, 100 ul, and 600 ul electroporation tubes; yeast cells: 20 ul, 100 ul, 200 ul, and 1000 ul electroporation tubes.
UHV-Plus (Ultra High Voltage), bacteria cells: 20 ul, 100 ul, and 600 ul electroporation tubes; yeast cells: 20 ul, 100 ul, 200 ul, and 1000 ul electroporation tubes; mammalian cells: 20 ul and 200 ul electroporation tubes.
Related searches:
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transfection with electroporation in CHO cells
CHO cell eletroporation transfection efficiency
CHO cell transfection efficiency
CHO electroporation transfection for antibody transient production
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transient gene expression (TGE).
Frequent questions related to “What is the difference between CHO chemical transfection and CHO electroporation transfusion?”:
What is the problem with transient transfection to CHO cells?
What cell type is appropriate for electroporation?
Is cell transformation and transfection the same?
Can the electroporation model SLT be used for transfection to other mammalian cells?
How successful is electroporation?
Is electroporation a physical method?
What is the mechanism of electroporation?
How can you increase the efficiency of electroporation?
Is electroporation better than chemical transformation?
What are the advantages and disadvantages of electroporation?
