What is the problem with transient transfection to CHO cells?
Question: What is the problem with transient transfection to CHO cells?
Answer: Both chemical transfection and electroporation-based transfection methods have been tried to improve the efficiency and yield of protein transient production in CHO cells. So far, the efficiency of most reagents and kits is low for chemical transfection method for CHO cells; the exception is the ExpiCHO system. However, the cost of the ExpiCHO system is higher compared with the other reagents and kits in the market. The volume of most CHO electroporation systems for transient production is low (100 ul); the exceptions are the Maxcyte system and the SLT system. However, the cost of the Maxcyte system is higher compared with our CHO electroporation model SLT for transient production and the consumables. The SLT system is the most economic solution for higher titer antibody transient production in the scale from 4 ml to a few liters.
Celetrix electroporation machines:
Cell electroporation basic model SP100, simplified single pulse for all cell types; for 20 ul, and 100 ul electroporation tubes
Cell electroporation upgrade model LE+, new cell line mode and PBMC mode. for 20 ul, 100 ul, 120 ul, and 200 ul electroporation tubes.
Cell electroporation upgrade model EX+, new cell line mode and PBMC mode. for 20 ul, 100 ul, 120 ul, 200 ul, 600 ul, and 1 ml electroporation tubes.
Cell electroporation large-scale model SLT, great for large scale CHO electroporation transfection for protein (antibody) transient production. for 200 ul, 1 ml, 5 ml, and 10 ml electroporation tubes.
Cell electroporation upgrade model EX+ with the electrofusion function, for 20 ul, 100 ul, 120 ul, 200 ul, 400 ul, 600 ul, and 1 ml electroporation tubes. Model EX+ and Model SLT can be equipped with optional fusion accessories for hybridoma generation. Mouse spleen cells can be fused with myeloma cells such as SP2/0 and P3X63Ag8 with high efficiency.
UHV Transformer (Ultra High Voltage), bacteria cells: 20 ul, 100 ul, and 600 ul electroporation tubes; yeast cells: 20 ul, 100 ul, 200 ul, and 1000 ul electroporation tubes.
UHV-Plus (Ultra High Voltage), bacteria cells: 20 ul, 100 ul, and 600 ul electroporation tubes; yeast cells: 20 ul, 100 ul, 200 ul, and 1000 ul electroporation tubes; mammalian cells: 20 ul and 200 ul electroporation tubes.
Related searches:
CHO electroporation transfection
transfection with electroporation in CHO cells
CHO cell eletroporation transfection efficiency
CHO cell transfection efficiency
CHO electroporation transfection for antibody transient production
CHO electroporation transfection for protein transient production
cho cell transient transfection
CHO electroporation transient production
antibody transient production in CHO
scalable CHO cell transfection
transient gene expression (TGE)
Frequent questions related to “What is the problem with transient transfection to CHO cells?”:
What equipment is used for electroporation?
What cell type is appropriate for electroporation?
How does electroporation affect cells?