Electrofusion Machine for Hybridoma Development
An electrofusion system for hybridoma development includes an electrofusion machine and compatible eletroporation tubes. A couple of Celetrix electroporation models, EX+ and SLT, can be equipped with optional fusion accessories for hybridoma generation with high-efficiency and low-toxicity. It can maintain a high level of cell survival.
Electro Cell Fusion for Hybridoma Production: The resulting local perforation of the cell membrane induces the cells to fuse, forming hybridomas. Electrofusion is accomplished in three steps: Prealignment of the cells (convergence and cell contact), membrane fusion, and postalignment (rounding off the fused cells). This method has been applied successfully to hybridoma production with higher efficiency than routine polyethylene glycol fusion, allowing production of more hybrid cells.
Celetrix electrofusion machines for hybridoma development:
Cell electroporation large-scale model SLT, great for large scale T cell electroporation. for 200 ul, 1 ml, 5 ml, and 10 ml electroporation tubes.
Cell electroporation upgrade model EX+ with the electrofusion function, for 20 ul, 100 ul, 120 ul, 200 ul, 400 ul, 600 ul, and 1 ml electroporation tubes. Mouse spleen cells can be fused with myeloma cells such as SP2/0 and P3X63Ag8 with high efficiency.
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