What is an isotype control antibody and why is it used?
Question: What is an isotype control antibody and why is it used?
Answer: An ideal isotype control matches the isotype of the primary antibody used in the application but lacks binding to the target. It is normally used as a negative control to differentiate, if there is any, non-specific background signal from the specific antibody signal. Mouse IgG1 kappa isotype controls against KLH, TNP, DNP, GST, HEL, His-tag, Flag, c-myc, HA, and GFP meet the requirements in some cases but do not in specific cases in the presence of the targets or cross-reactivity.
Syd Labs provides in vivo grade Recombinant IgG Isotype Control Antibodies and Mutants:
In Vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody
In Vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody, Endotoxin 0.05 EU/mg
In Vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody, Endotoxin 0.05 EU/mg
In Vivo Grade Recombinant Mouse IgG1-D265A Isotype Control Antibody, Endotoxin 0.05 EU/mg
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Mouse IgG1 kappa isotype controls
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Is your mouse IgG1 isotype control also Mouse IgG1, kappa monoclonal (MOPC-21) – isotype control?