Guaranteed Mouse Monoclonal Antibody Production Services

Catalog No.	BS010
Product Name	Guaranteed Mouse Monoclonal Antibody Production Services
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Summary	hybridoma-based custom mouse monoclonal antibody development services: production of anti-peptide, protein, chemical, cell monoclonal antibodies; DNA immunization; RIMMS; monoclonal antibodies for ELISA, Western blot, IHC, flow cytometry, diagnostics and therapeutics.
Note	The prices are for custom mouse monoclonal antibody production projects completed in China. The cost for production of custom monoclonal antibodies including immunogen preparation and mice immunization are higher if completed in US.
Order Offline	Phone: 1-617-401-8149Fax: 1-617-606-5019Email: message@sydlabs.comOr leave a message with a formal purchaseorder (PO) Or credit card.

Description

The most comprehensive hybridoma-based mouse monoclonal antibody production services:
custom production of monoclonal antibodies by hybridoma technology against non-modified, phospho-specific, methylation-specific, and acetylation-specific peptides and proteins as well as small molecular compounds; for ELISA, Western blot, IHC, and flow cytometry applications.

Hybridoma generation is the oldest and the most cost-effective method for monoclonal antibody development. It has been successfully used with mouse, rat, hamster, rabbit, sheep, and human. Our experience is mostly from mice. The workflow consists of: animal immunization, cell fusion with PEG or electro, screening antibodies with applications of ELISA, flow cytometry, tissue microarray, and such, and then hybridoma subcloning and expansion. It was reported that the electro fusion method gave 10 times of clones out of the PEG-based hybridoma fusion method. Screening of antibodies for various applications may be done by CROs like Syd Labs or clients themselves. After the number of hybridoma clones narrows down, antibody activity is confirmed by clients or in house during antibody screening and hybridoma subcloning.

BS010A: $6,000 for a standard 24-week process of mouse immunization and hybridoma development following the standard protocol of mouse monoclonal antibody development.
Phase I, mouse immunization: Immunization and boosts of 5 Balb/c mice following the standard immunization protocol and ELISA screening of mouse polyclonal sera.
Phase II, spleen fusion: Fusion of splenocytes to mouse myeloma cells and ELISA screening to isolate antigen-positive hybridoma cells from an initial count of approximately 3000 fusion clones. Supernatant (conditioned media) from 10 of the final 2-3 ELISA-positive clone population pools will be sent to clients for evaluation of specific applications.
Phase III, hybridoma development: Subcloning of the ELISA-positive clone population pools by limited dilution to isolate individual hybridoma clones of interest, and expansion of the individual hybridoma cell lines.

You provide: Ten mg of peptide (purity > 85%, length > 12 amino acids) or 4 mg of soluble protein (concentration > 0.4 mg/ml, purity > 90%, molecular weight > 20 kDa) for immunization, and additional 5 mg for antigen affinity purification.

Deliverable:
* About 200 ul supernatant from 10 ELISA positive hybridoma cells for evaluation;
* 1 positive hybridoma cell line;
* Remaining antigens;
* ELISA results.

The highest quality standard in the field of mouse monoclonal antibody production services: guaranteed ELISA titer > 1:40 000.

The prices of custom mouse monoclonal antibody development services will be adjusted for the following requirements:

a. Peptide (12-15 amino acids, >85%) design and synthesis +$600 for standard, +$900 for mono-phosphorylated, +$950 for di-phosphorylated, +$1000 for mono-methylated (K or R site), +$1050 for di-methylated (K or R site), +$1200 for tri-methylated (K site), +$700 for acetylated;
b. Protein: DNA subcloning +$500, bacterial expression and purification +$3,000;
c. Additional boost for higher ELISA titer of the antisera +$200/injection;
d. Additional positive hydridoma cell line +$600.

Questions and answers:

Question: What are the most important parts in custom monoclonal antibody development?

Answer: No matter if clients develop monoclonal antibodies in house or by CRO, one must have two things: one is a reliable high-throughput assay to evaluate antibody candidates, and the other great results of animal immunization. Depending on clients’ antibody application, one needs to develop: functional assays for therapeutic application, immunoassay with real samples for clinical diagnostics application, and tissue microarray for reagent development.

References:

1. “Mouse monoclonal antibodies 1G, 6A, and 10D were produced for this project by Syd Labs, Inc. (Natick, MA) using full-length recombinant human acetylcholinesterase (rHuAChE) as immunogen. Large quantities of monoclonal antibodies 1G, 6A, and 10D were produced in mouse ascites fluid and purified by Syd Labs, Inc.” Immunopurification of Acetylcholinesterase from Red Blood Cells for Detection of Nerve Agent Exposure.

2. “Antibodies were generated in mice by Syd Labs, Inc. using standard hybridoma fusion technology.” Monoclonal Antibody That Recognizes Diethoxyphosphotyrosine-Modified Proteins and Peptides Independent of Surrounding Amino Acids.

Syd Labs also provides other monoclonal antibody development services:

Rabbit monoclonal antibody development services
Alpaca/llama monoclonal antibody development services

After great hybridoma clones have been developed, if clients want to file patents, protect the antibodies using the digital format, or develop antibody drugs, clients can ask Syd Labs to clone and sequence the hybridoma antibody variable regions (VH and VL), do transient expression of chimeric antibodies (scFv or complete IgG) in CHO or HEK293 cells, or generate stable cell line expressing antibodies.

Additional information

Ship from Country	USA
Size	1 package

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