Guaranteed Rat Monoclonal Antibody Development Services
The most comprehensive rat custom monoclonal antibody development services: custom production of monoclonal antibodies against non-modified, phospho-specific, methylation-specific, and acetylation-specific peptides and proteins; custom monoclonal antibodies for ELISA, Western blot, IP, IF, ICC, and IHC applications. Guaranteed ELISA > 1:40 000.
- Product Details
- Description
- Additional information
- More Offers
| Catalog No. | BS009 |
|---|---|
| Product Name | Guaranteed Rat Monoclonal Antibody Development Services |
| Supplier Name | Syd Labs, Inc. |
| Brand Name | Syd Labs |
| Summary | The most comprehensive rat custom monoclonal antibody development services: custom production of monoclonal antibodies against non-modified, phospho-specific, methylation-specific, and acetylation-specific peptides and proteins; custom monoclonal antibodies for ELISA, Western blot, IP, IF, ICC, and IHC applications. Guaranteed ELISA > 1:40 000. |
| Note | The lowest prices are guaranteed. If you find any US company providing the same quality services with lower prices, we will return the difference to you. |
| Order Offline | Phone: 1-617-401-8149Fax: 1-617-606-5019Email: message@sydlabs.comOr leave a message with a formal purchaseorder (PO) Or credit card. |
Description
The most comprehensive rat monoclonal antibody development services: custom production of monoclonal antibodies against non-modified, phospho-specific, methylation-specific, and acetylation-specific peptides and proteins; monoclonal antibodies for ELISA, Western blot, IP, IF, ICC, and IHC applications.
BS009A: $7,500 for a standard 24-week hybridoma development program.
Phase I: Immunization and boosts of 5 rats following the standard immunization protocol.
Phase II: Fusion and ELISA screening. Out of 1,200 to 3,000 cells screened, about 300 microliter supernatants from 10 ELISA positive cells will be sent to customers for evaluation.
Phase III: Subcloning and expansion. All clones selected by the customer will be subcloned by limiting dilution, isotyped, and expanded.
You provide: Ten mg of peptide (purity > 85%, length > 12 amino acids) or 4 mg of soluble protein (concentration > 0.4 mg/ml, purity > 90%, molecular weight > 20 kDa) for immunization, and additional 5 mg for antigen affinity purification.
Deliverable:
* 50 ul of pre-immune serum;
* About 200 ul supernatant from 10 ELISA positive cells for evaluation;
* 1 positive hybridoma cell lines;
* Remaining antigens;
* ELISA results.
The highest quality standard in the field of mouse monoclonal antibody production services: guaranteed ELISA > 1:40,000.
Clients have the choice for licensing antibodies to save cost and even make money.
The prices will be adjusted for the following requirements:
a. Peptide (12-15 amino acids, >85%) design and synthesis +$600 for standard, +$900 for mono-phosphorylated, +$950 for di-phosphorylated, +$1000 for mono-methylated (K or R site), +$1050 for di-methylated (K or R site), +$1200 for tri-methylated (K site), +$700 for acetylated;
b. Rat monoclonal antibody production service for modified peptide: +$3,000 for phospho-specific, +$3,000 for methylation-specific, +$3,000 for acetylation-specific;
c. Synthesis of other antigens, +$variable;
d. Conjugation of antigens to protein carriers +$250;
e. Protein: DNA subcloning +$500, bacterial expression and purification +$3,000;
f. Additional boost immunization for higher ELISA titer of the antisera +$200/injection;
g. Additional positive hydridoma cell line +$600;
h. Antibody purification: antigen affinity purification +$500, protein A/G affinity purification +$700, ammonium sulfate precipitation +$400;
i. Guaranteed applications: Western blot +$5,000, IP +$5,000, IF +$6,000, ICC +$6,000, IHC +$6,000.
Additional information
| Ship from Country | USA |
|---|---|
| Size | 1 package |