Induced Pluripotent Stem Cells (iPSCs) Electroporation Systems
An Induced Pluripotent Stem Cells (iPSCs, iPSC, or iPS cells) electroporation system includes an electroporation machine and compatible electroporation tubes. A few Celetrix electroporation models can be used for iPS stable cell line development as well as transient transfection into iPS cells. Transposon tools are available to increase transfection efficiency.
Contemporary Transposon Tools: A Review and Guide through Mechanisms and Applications of Sleeping Beauty, piggyBac and Tol2 for Genome Engineering: Transposons enable several avenues for genome manipulations in vertebrates, including transgenesis for the generation of transgenic cells in tissue culture comprising the generation of pluripotent stem cells, the production of germline-transgenic animals for basic and applied research, forward genetic screens for functional gene annotation in model species and therapy of genetic disorders in humans.
Celetrix iPS cell electroporation machines:
Cell electroporation basic model SP100, simplified single pulse for all cell types; for 20 ul, and 100 ul electroporation tubes
Cell electroporation upgrade model LE+, new cell line mode and PBMC mode. for 20 ul, 100 ul, 120 ul, and 200 ul electroporation tubes.
Cell electroporation upgrade model EX+, new cell line mode and PBMC mode. for 20 ul, 100 ul, 120 ul, 200 ul, 600 ul, and 1 ml electroporation tubes.
Cell electroporation large-scale model SLT, great for large scale CHO electroporation transfection for protein (antibody) transient production. for 200 ul, 1 ml, 5 ml, and 10 ml electroporation tubes.
Cell electroporation upgrade model EX+ with the electrofusion function, for 20 ul, 100 ul, 120 ul, 200 ul, 400 ul, 600 ul, and 1 ml electroporation tubes. Model EX+ and Model SLT can be equipped with optional fusion accessories for hybridoma generation. Mouse spleen cells can be fused with myeloma cells such as SP2/0 and P3X63Ag8 with high efficiency.
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Frequent questions related to “iPS cell electroporation system”:
Why are iPS cells used for transfection?
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