What are the published applications of the 29F.1A12 antibody?
Question: What are the published applications of the 29F.1A12 antibody?
Answer: It was reported that the blockade of the PD-1 protein expressed on TME-infiltrating macrophages and natural killer (NK) cells by 29F.1A12 results in tumor growth suppression and prolonged survival. It is also reported that the 29F.1A12 antibody recognizes the PD-1 surface protein on B16-F10 and other murine tumor lines, suppresses cancer cell-intrinsic PD-1 receptor signaling, and inhibits resultant tumor growth in three-dimensional (3D) culture and tumor-bearing mice. In the paper “Distinct antibody clones detect PD-1 checkpoint expression and block PD-L1 interactions on live murine melanoma cells“, it was shown that the anti-PD-1 29F.1A12 monoclonal antibody recognizes the PD-1 surface protein on live B16-F10 melanoma cells, live YUMM melanoma variant lines, and overlapping WT murine B16-F10 and YUMM melanoma and T-cell subsets by FACS. In contrast to the RMP1-30 antibody, The 29F.1A12 PD-1 blocking antibody inhibits PD-1 interactions with PD-L1 on live B16-F10 melanoma cells. The RMP1-14 clone, but not 29F.1A12 or RMP1-30, is useful for pulling down (IP) and sequencing PD-1 from B16-F10 and T-cell cultures, consistent with the previous observations that individual antibodies differ in their suitability for specific assay types. 29F.1A12 binding to B16-F10 cells was increased in 3D versus 2D settings.
Syd Labs provides the following anti-mouse PD-L1 / PD-1 antibodies:
In Vivo Grade Recombinant Anti-mouse PD-1 Mouse IgG2c Kappa Monoclonal Antibody (Clone 29F.1A12.1)
In Vivo Grade Recombinant Anti-mouse PD-1 Rat IgG2a Kappa Monoclonal Antibody (Clone 29F.1A12.1)
In Vivo Grade Recombinant Anti-mouse PD-1 Rabbit IgG Monoclonal Antibody (Clone 29F.1A12.1)
In Vivo Grade Recombinant Anti-mouse PD-1 Mouse IgG2a Kappa Monoclonal Antibody (Clone 29F.1A12.1)
In Vivo Grade Recombinant Anti-mouse PD-1 Mouse IgG1 Kappa Monoclonal Antibody (Clone 29F.1A12.1)
Related searches:
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anti-PD-1 29F.1A12 monoclonal antibody
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What are the published applications of the 29F.1A12 antibody?
