Can the electroporation model SLT be used for stable transfection in addition to transient transfection?
Question: Can the electroporation model SLT be used for stable transfection in addition to transient transfection?
Answer: What results in the difference between transient transfection and stable transfection? Transient transfection exerts a temporary influence on cells because the external DNA fragments are not integrated to the host genome, whereas stable transfection leads to permanent genetic changes, by DNA integration into the host genome, that are usually passed on to future cell progeny. The plasmid vectors used make the major difference. The electroporation machine SLT can also be used to optimize CHO stable transfection.
Celetrix electroporation machines:
Cell electroporation basic model SP100, simplified single pulse for all cell types; for 20 ul, and 100 ul electroporation tubes
Cell electroporation upgrade model LE+, new cell line mode and PBMC mode. for 20 ul, 100 ul, 120 ul, and 200 ul electroporation tubes.
Cell electroporation upgrade model EX+, new cell line mode and PBMC mode. for 20 ul, 100 ul, 120 ul, 200 ul, 600 ul, and 1 ml electroporation tubes.
Cell electroporation large-scale model SLT, great for large scale CHO electroporation transfection for protein (antibody) transient production. for 200 ul, 1 ml, 5 ml, and 10 ml electroporation tubes.
Cell electroporation upgrade model EX+ with the electrofusion function, for 20 ul, 100 ul, 120 ul, 200 ul, 400 ul, 600 ul, and 1 ml electroporation tubes. Model EX+ and Model SLT can be equipped with optional fusion accessories for hybridoma generation. Mouse spleen cells can be fused with myeloma cells such as SP2/0 and P3X63Ag8 with high efficiency.
UHV Transformer (Ultra High Voltage), bacteria cells: 20 ul, 100 ul, and 600 ul electroporation tubes; yeast cells: 20 ul, 100 ul, 200 ul, and 1000 ul electroporation tubes.
UHV-Plus (Ultra High Voltage), bacteria cells: 20 ul, 100 ul, and 600 ul electroporation tubes; yeast cells: 20 ul, 100 ul, 200 ul, and 1000 ul electroporation tubes; mammalian cells: 20 ul and 200 ul electroporation tubes.
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transient gene expression (TGE).
Frequent questions related to “Can the electroporation model SLT be used for stable transfection in addition to transient transfection?”:
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