Mycobacteria DNA Detection Kit
HZSKBIO Mycobacteria DNA Detection Kit (1503602) is used, together with Mycobacteria DNA Extraction Kit, to qualitatively determine whether there is Mycobacteria contamination in cells, cell culture-derived biologicals, etc., using real-time qPCR techonology.
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| Catalog No. | 1503602 |
|---|---|
| Product Name | Mycobacteria DNA Detection Kit |
| Supplier Name | HZSKBIO |
| Brand Name | MycoSHENTEKTM |
| Shipping | Shipped at Room Temp. |
| Order Offline | Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Orleave a message with a formal purchase order (PO) Or credit card. |
Description
1503602: MycoSHENTEKTM Mycobacteria DNA Detection Kit
Product Overview
MycoSHENTEKTM Mycobacteria DNA Detection Kit(catalog number: 1503602) is used, together with MycoSHENTEKTM Mycobacteria DNA Extraction Kit, to qualitatively determine whether there is Mycobacteria contamination in cells, cell culture-derived biologicals, etc., using real-time qPCR techonology. The integrated system has been fully validated at a detection limit of 10-100 CFU/mL to detect more than 100 different Mycobacteria species. The system contains dUTP, and if used with the UNG enzyme to prevent contamination and eliminate carry-over contamination Internal Control (IC) can be added to a PCR amplification reaction mixture to evaluate whether a test sample inhibits the amplification reaction and may result in false negative results. It can also be added to the sample before extraction to evaluate the extraction efficiency. For extraction information, please refer to MycoSHENTEKTM Mycobacteria DNA.
Specification
Recovery：at least 90% to fg/μL
Repeatability：CV no more than 15%
Mycobacteria DNA Extraction Kit contents and storage
| Reagent | Part No. | Quantity | Storage |
| MB qPCR Reaction Buff | NNB011 | 400 μL × 1 tube | -20℃, protect from light |
| M protect from light B Primer & Probe MIX | NNC035 | 75 μL × 1 tube | |
| MB Internal Control (IC) | NNA037 | 600 μL × 1 tube | -20℃ |
| MB Positive Control (PC) | NNA041 | 500 μL × 1 tube | |
| DNA Dilution Buffer(DDB) | NND001 | 1.5 mL × 1 tube |
The kit components can be stored at appropriate conditions for up to 24 months.
Applied instruments, including but not limited to the following
SHENTEK-96S Real-time PCR System
CFX96 Real-Time PCR System
7500 Real-Time PCR Sys
Materials required (Not included in the Kit)
Nonstick, RNase-free microfuge tubes, 1.5mL, 2.0mL
PCR 8-well strip tubes with caps or 96-well plates with seals
Low retention filter tips 1000μL, 100μL, 10μL
75% Ethanol
UNG enzyme (Please check the effective amount for best use.)
Related equipment
Laminar flow cabinet or biosafety cabinet
Benchtop microcentrifuge & PCR strip/plate centrifuge
Microplate and micro test tube shaker
Vortex mixer
Real-time PCR System
Pipettes, 1000μL, 100μL, 10μL
Please remember our product information: Mycobacteria DNA Detection Kit catalog number: 1503602 MycoSHENTEKTM.
Additional information
| Ship from Country | USA |
|---|---|
| Size | 50 Reactions |