Mycoplasma DNA Extraction Kit (2G)
HZSKBIO Mycoplasma DNA Extraction Kit (2G) (1509840) is used for extraction of trace mycoplasma DNA from master cell banks, working cell banks, virus seed banks, and complex matrices such as 107 cells culture, 5% human albumin culture solution, or high-concentration plasmids, etc.
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| Catalog No. | 1509840 |
|---|---|
| Product Name | Mycoplasma DNA Extraction Kit (2G) |
| Supplier Name | HZSKBIO |
| Brand Name | MycoSHENTEK® |
| Shipping | Shipped at Room Temp. |
| Order Offline | Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Orleave a message with a formal purchase order (PO) Or credit card. |
Description
1509840: MycoSHENTEK® Mycoplasma DNA Extraction Kit (2G)
Product Overview
MycoSHENTEKTM Mycoplasma DNA Extraction Kit (2G) is used for extraction of trace mycoplasma DNA from master cell banks, working cell banks, virus seed banks, and complex matrices such as 107 cells culture, 5% human albumin culture solution, or high-concentration plasmids, etc. It works with MycoSHENTEKTM Mycoplasma Detection Kit (2G) for an integrated workflow from sample preparation to detection assay. The integrated system has been fully validated per European Pharmacopoeia Chapter 2.6.7 and Japanese Pharmacopoeia XVII, at a detection limit of 10 CFU/mL. For sample volume that is less than 400 μL, test sample DNA can be extracted directly using Mycoplasma DNA Extraction Kit (2G). If it is necessary to increase the sample volume to achieve a higher detection sensitivity, the sample should be concentrated by centrifugation to a final volume of approximately 400 μL before using this extraction kit. The kit is compatible with manual sample preparation, or with automated extraction using rHCDpurify instrument.
Specification
Recovery：at least 90% to fg/μL
Repeatability：CV no more than 15%
Mycoplasma DNA Extraction Kit (2G) contents and storage
| No | Reagent | Part No. | Quantity | Storage |
| I | Wash buffer A | NND015 | 15 mL × 1 bottle | room temperature |
| Binding solution | NND017 | 10 mL × 1 bottle | ||
| Elution buffer | NND019 | 5 mL × 1 bottle | ||
| Dilution buffer | NND021 | 10 mL × 2 bottle | ||
| Lysis buffer | NND028 | 5 mL × 1 bottle | ||
| Pretreatment buffer | NND022 | 5 mL × 1 bottle | ||
| II | Magnetic particles | NND002 | 1.25 mL × 4 tubes | 2-8℃ |
| 5M NaCl | NND033 | 750 μL × 2 tubes | ||
| Precipitation solution I | NND040 | 500 μL × 1 tube | ||
| Ⅲ | Precipitation solution II | NND003 | 25 μL × 1 tube | -20℃ |
| Proteinase K | NND004 | 500 μL × 1 tube | ||
| Wash buffer A | NND023 | 500 μL × 2 tubes |
The kit components can be stored at appropriate conditions for up to 24 months.
Materials required (Not included in the Kit)
ddH2O
Anhydrous Ethanol (AR)
100% Isopropanol(AR)
PCR 8-well strip tubes with caps or 96-well plate with seals
Nonstick，Low Retention tips 1000μL, 100μL, 10μL
Nonstick, RNase-free microfuge tubes 1.5mL, 2.0mL, 50mL
Related equipment
Benchtop microcentrifuge
Magnetic stand or rHCDpurify
High-speed refrigerated centrifuge
Vortex mixe
Dry bath incubator
Pipettors,1000μL, 100μL, 10μL
Real-time PCR instrument
Biosafety cabinet
Microplate and microtube shaker
Please remember our product information: Mycoplasma DNA Extraction Kit (2G) catalog number: 1509840 MycoSHENTEK®.
Additional information
| Ship from Country | USA |
|---|---|
| Size | 50 Extractions |