UltraScence Pico Ultra Western Substrate
$88.00
BIO-HELIX UltraScence Pico Ultra Western Substrate (catalog No. CCH345-B100ML), Chemiluminscent HRP substrate for Western Blotting (detection sensitivity from low picogram to mid femtogram).
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| Catalog No. | CCH345-B100ML |
|---|---|
| Product Name | UltraScence Pico Ultra Western Substrate |
| Supplier Name | BIO-HELIX |
| Brand Name | BIO-HELIX |
| Synonyms | Enhanced Chemiluminescent / HRP (Horseradish Peroxidase) / ECL substrates / Picogram / Femtogram / Luminol / Peroxide Buffer / Western Blotting |
| Order Offline | Syd Labs, Inc. 4 Avenue E, Hopkinton, MA 01748 USA. Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com |
Description
CCH345-B100ML: UltraScence Pico Ultra Western Substrate
The UltraScence Pico Ultra Western Substrate, as a luminol-based enhanced chemiluminescent substrate, is sensitive and compatible with conducting immunoblots with horseradish peroxidase (HRP) – conjugated secondary antibodies. The low picogram to mid femtogram detection of antigen is enabled by UltraScence Pico Ultra Western Substrate’s excellent sensitivity and long signal duration. Further, its long chemiluminescent signal duration makes both digital and film-based imaging possible without any loss of the signal. Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration.
Product Name: UltraScence Pico Ultra Western Substrate
Product Item No.: CCH345-B100ML
Dimensions: 100ML
Substrate Type: HRP (Horseradish Peroxidase) Substrate
Contents/Storage: Store at 4℃ for 2 years
**US Patent No.: US10,711,185
Features:
– No optimization required.
Switching to theUltraScence Pico Ultra Western Substrate from other brands, such as Thermo Scientific™ SuperSignal™ West Dura and Millipore™ Immobilon™ substrates, does not require optimization or protocol changes.
– High degree of sensitivity and enhanced chemiluminescence duration.
UltraScence Pico Ultra Western Substrate enables an accurate low picogram to mid femtogram detection of protein on the same immunoblot after a single exposure.
– Optimized for use with PVDF and nitrocellulose membranes.
– Compatible with Western Blotting Markers.
– Optimized for film- and CCD-based imaging.
– Patent pending product.
**US Patent No.: US10,711,185
Chemiluminescent Development
1. Keep the membrane moist in the wash buffer while preparing the substrate mixture. Make sure the membrane does not dry out in the next steps.
2. The chemiluminescent substrate solution is sufficiently stirred to prepare the 0.1ml of solution / cm2 of the membrane.
– For a small membrane (7 x 8.5 cm), 4 ml of solution is sufficient.
– For a medium-sized membrane (8.5 x 13.5 cm), 10 ml of solution is sufficient.
3. Place the membrane on a clean, flat surface or in a clean container.
4. Remove the membrane from the chemiluminescent substrate solution and drain the excess solution.
5. Place the membrane in a plastic sheet protector or plastic wrap to prevent the film from drying out.
6. Imaging the membrane with a digital imager or by exposure to the X-ray film.
FAQ
Q: From Bio-Helix’s UltraScence ECL substrates portfolio, which level of ECL fits best my Western blot applications?
A: UltraScence ECL substrates series is compatible with the use from low picogram to low-femtogram level detections. Please kindly refer to the ECL selection guide of UltraScence Western substrate as the below table:
(a) CCH321-B100ML, UltraScence Pico Plus Western Substrate
(b) CCH345-B100ML, UltraScence Pico Ultra Western Substrate
(c) CCH365-B100ML, UltraScence Femto ECL Western Substrate
(d) CCH375-B100ML, UltraScence Femto Plus ECL Western Substrate
(e) CCH385-B100ML, UltraScence Atto Western Substrate
References
▋GROZAVU, Ingrid, et al. D154Q mutation does not alter KRAS dimerization. Journal of Molecular Biology, 2022, 434.2: 167392.
▋LYONS, Bronwyn JE, et al. Cryo-EM structure of the EspA filament from enteropathogenic Escherichia coli: revealing the mechanism of effector translocation in the T3SS. Structure, 2021, 29.5: 479-487. e4.
▋MALARD, Camille. Efficacy, safety, and delivery of anti-HIV short-hairpin RNA molecules for use in HIV gene therapy. McGill University (Canada), 2019.
Please remember our product information: UltraScence Pico Ultra Western Substrate catalog number: CCH345-B100ML BIO-HELIX
Additional information
| Ship from Country | USA |
|---|---|
| Size | 100 ml |