How does the luciferin luciferase system work?
Question: How does the luciferin luciferase system work?
Answer: The luciferin luciferase reaction mechanism is well known. Light can be generated by oxidation of D-luciferin but the reaction rate is extremely slow in absence of the enzyme and the cofactor ATP. The enzyme catalyzes the chemical reaction of its substrate to produce light. The other chiral form, L-luciferin, competitively inhibits the chemical reaction. The luciferin luciferase system has been widely used in in vitro cell-based assays and in vivo animal models.
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