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Biotinylated Anti-Human HLA Class I Heavy Chain Antibody (HC10, Human IgG1 Fc Silent Kappa)

Name: Biotinylated Anti-Human HLA Class I Heavy Chain Human IgG1 Fc Silent Kappa | Ushelf
Brand: Syd Labs
SKU: PA007763.h1Fs-b
Price: 300-600 USD
Availability: InStock
Rating: 5 (15 reviews)

Catalog No.: PA007763.h1Fs-b | Isotype: Human IgG1 Fc Silent Kappa | Flow Cytometry

$300.00 – $600.00

Recombinant anti-human HLA Class I Heavy Chain monoclonal antibody (Clone HC10), engineered with a Human IgG1 (Fc-Silent) domain and conjugated to Biotin. Variable regions are derived from the classic mouse anti-human HLA class I heavy chain clone (HC10) and recombinantly expressed in mammalian cells to minimize non-specific Fc-receptor binding during flow cytometry analysis.

Endotoxin Level: < 1.0 EU/mg
Applications: Flow Cytometry (FC), Cell Surface Staining, Functional Assays
Alternative Formats: Unconjugated, alternative Fc isotypic engineered variants, and fluorophore-conjugated versions of clone HC10 are also available.

Details & Specifications
References

Catalog No.	PA007763.h1Fs-b
Product Name	Biotinylated Recombinant Anti-Human HLA Class I Heavy Chain Monoclonal Antibody, Human IgG1 Fc Silent Kappa (Clone: HC10)
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Clone	HC10.
Isotype	Human IgG1 Fc Silent Kappa.
Source/Host	The site-directed biotinylated anti-human HLA Class I Heavy Chain monoclonal antibody (clone: HC10) was produced in mammalian cells.
Specificity/Sensitivity	The site-directed biotinylated recombinant HC10 antibody specifically binds to human HLA Class I Heavy Chain.
Applications	Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form Of Antibody	0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Conjugate	Biotin-conjugated.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The HC10 antibody are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant mouse IgG2a isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007763.h1Fs-b: Biotinylated Recombinant Anti-Human HLA Class I Heavy Chain Monoclonal Antibody, Human IgG1 Fc Silent Kappa (Clone: HC10)

Recombinant human IgG1 Monoclonal Antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.

References of Anti-Human HLA Class I Heavy Chain Monoclonal Antibody (Clone: HC10):

1. Endo-lysosomal assembly variations among human leukocyte antigen class I allotypes

Olson, E., et al. Elife. 2023;12:e79144. PMID: 36722462

“The HC10 monoclonal antibody (anti-human HLA Class I Heavy Chain) was used in peptide receptivity assays to measure specific expression and localization of HLA-B allotypes in donors selected from a genotyped cohort. Anti-Bw6 and anti-Bw4 antibodies, including HC10, facilitated analysis of homozygous or heterozygous HLA-B allotypes, ensuring minimal cross-reactivity with HLA-A or HLA-C allotypes.”

2. Non-conventional forms of HLA-B27 are expressed in spondyloarthritis joints and gut tissue

Rysnik, O., et al. J Autoimmun. 2016;70:12-21. PMID: 27036372

“The HC10 antibody (anti-human HLA Class I Heavy Chain) and HD6 were used to detect non-conventional HLA-B27 (NC-B27) expression in human SpA joints, gut, and B27 TG 1 rat tissues via immunohistochemistry, flow cytometry, and confocal microscopy. High NC-B27 levels were observed in infiltrating and resident cells of inflamed joints and spleens of B27 TG 1 rats, increasing with age and disease progression.”

3. HLA-B27-Homodimer-Specific Antibody Modulates the Expansion of Pro-Inflammatory T-Cells in HLA-B27 Transgenic Rats

Marroquin Belaunzaran, O., et al. PLoS One. 2015;10(6):e0130811. PMID: 26125554

“The HC10 monoclonal antibody (anti-human HLA Class I Heavy Chain, IgG2a) served as a positive control, binding β2m-free heavy chains of HLA-B and -C, including B272 and B27-β2m-free forms. Flow cytometry with HC10, HD6, and other antibodies (0.02 mg/mL) on LBL721.220 cells and leukocytes from transgenic rats confirmed cell-surface B272 expression, with secondary detection using PE-conjugated goat anti-mouse IgG.”

4. The Leukocyte Immunoglobulin-Like Receptor Family Member LILRB5 Binds to HLA-Class I Heavy Chains

Jones, D. C., et al. PLoS One. 2015;10(6):e0129063. PMID: 26098415

“The HC10 monoclonal antibody (anti-human HLA Class I Heavy Chain) blocked B27 dimer tetramer binding to LILRB5-transfected 293T cells, as confirmed by flow cytometry with W6/32, HC10, and ME1 antibodies. HC10 and anti-LILRB5 antisera inhibited B27 dimer binding, while multiparameter flow cytometry assessed LILRB5 surface expression on lymphocyte and monocyte populations.”

5. Psoriatic patients with arthropathy show significant expression of free HLA class I heavy chains on circulating monocytes: a potential role in the pathogenesis of psoriatic arthropathy

Lan, C-C E, et al. Br J Dermatol. 2004;151(1):24-31. PMID: 15270869

“The HC10 monoclonal antibody (anti-human HLA Class I Heavy Chain) detected free HLA class I heavy chains on circulating monocytes via flow cytometry in patients with psoriatic arthropathy. Peripheral blood mononuclear cells, isolated by Ficoll-Hypaque centrifugation, were stained with HC10 followed by FITC-conjugated goat anti-mouse IgG and analyzed using a FACScan flow cytometer with CellQuest software.”

For more references about anti-human HLA Class I Heavy Chain antibody (HC10), please contact our scientific support team at message@sydlabs.com.

Ship from Country	USA
Size	100 ug 500 ug Clear

Biotinylated Anti-Human HLA Class I Heavy Chain Antibody (HC10, Human IgG1 Fc Silent Kappa)

Biotinylated Anti-Human HLA Class I Heavy Chain Antibody (HC10, Human IgG1 Fc Silent Kappa)