Anti-Mouse PD-1 (CD279) [RMP1-14] In Vivo Antibody | PA007162.rt

Name: Anti-Mouse PD-1 (CD279) [RMP1-14] In Vivo Antibody | Ushelf
Brand: Syd Labs
SKU: PA007162.rt
Price: 595 USD
Availability: InStock
Rating: 5 (12 reviews)

$595.00

In Vivo Grade Recombinant Anti-mouse PD-1 Rabbit IgG Monoclonal Antibody (Clone RMP1-14.1). Recombinant mouse anti-mouse PD 1 / CD279 monoclonal antibodies, whose variable region sequences are murined from the rat anti-mouse PD-1 monoclonal antibody (clone number: RMP1-14), are produced from mammalian cells. The recombinant rat and chimeric mouse versions of the RMP1-14 antibody are also available.

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Syd Labs, Inc.

SKU: PA007162.rt Categories: Anti-Mouse Antibodies (In Vivo), Antibodies Tags: Anti-mouse CD279 In Vivo Antibody, Anti-mouse PD-1, Anti-mouse PD-1 Antibody

Details & Specifications
References
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Catalog No.	PA007162.rt
Product Name	Anti-Mouse PD-1 (CD279) [RMP1-14] In Vivo Antibody \| PA007162.rt
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	Mouse Anti-Mouse PD 1 Monoclonal Antibodies, Murinized Anti-Mouse PD 1 Monoclonal Antibodies
Summary	The In Vivo Grade Recombinant Anti-mouse PD-1 Rabbit IgG Monoclonal Antibody (Clone RMP1-14.1) was produced in mammalian cells.
Clone	RMP1-14.1, the same variable region and constant region sequences as the rat anti-mouse PD-1 monoclonal antibody (clone number: RMP1-14)
Isotype	Rabbit IgG, kappa
Applications	immunohistochemistry (IHC), Flow Cytometry (FC), and various in vitro and in vivo functional assays.
Immunogen	The original rat hybridoma (clone name: RMP1-14) was generated by immunizing Sprague Dawley rats with mouse PD-1-transfected BHK cells and using a P3U1 myeloma as the fusion partner.
Form Of Antibody	0.2 μM filtered solution of 1x PBS.
Endotoxin	Less than 1 EU/mg of protein as determined by LAL method.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The In Vivo Grade Recombinant Anti-mouse PD-1 Rabbit IgG Monoclonal Antibody (Clone RMP1-14.1) are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 1 month from date of receipt, 2 to 8°C as supplied. 3 months from date of receipt, -20°C to -70°C as supplied.
Note	Recombinant mouse anti-mouse PD 1 / CD279 monoclonal antibodies, whose variable region sequences are murined from the rat anti-mouse PD-1 monoclonal antibody (clone number: RMP1-14), are produced from mammalian cells. The recombinant rat and chimeric mouse versions of the RMP1-14 antibody are also available.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007162.rt: Recombinant Anti-mouse PD-1 Monoclonal Antibody (RMP1-14.1), Rabbit IgG kappa, In Vivo Grade

References for Anti-Mouse PD-1 (CD279) [RMP1-14] In Vivo Antibody:

1、Anti-PD-1 antibody therapy potently enhances the eradication of established tumors by gene-modified T cells.
John, L. B., et al. Clin Cancer Res. 2013 Oct 15;19(20):5636-46. doi: 10.1158/1078-0432.CCR-13-0458. PMID: 23873688
In this study, we show that blockade of the PD-1 immunosuppressive pathway using an anti-PD-1 antibody can significantly enhance the antitumor efficacy of genetically modified T cells expressing a chimeric antigen receptor (CAR). …The PD-1 pathway has emerged as another promising target for cancer therapy. …Engagement of the PD-1/PD-L1 pathway results in the phosphorylation of tyrosine-based motifs in the cytoplasmic tail of the PD-1 inhibitory receptor, which promotes the recruitment of SHP2 phosphatase leading to dephosphorylation of PI3K. …Recent trials using a fully human IgG4 PD-1 monocloncal antibody (mAb; BMS-936558) have reported durable clinical responses in patients with advanced melanoma, non?small cell lung, renal cell, as well as hematologic malignancies. …Hence, in this study, we hypothesized that CAR T-cell therapy in combination with PD-1 blockade may overcome PD-L1+ tumor immunosuppression, thereby leading to improved therapeutic efficacy.
Tags: Anti-Mouse PD-1 (CD279) mAb of low endotoxin; clone RMP1-14 of invivo anti-mouse PD-1 antibody

2、Indoleamine 2,3-dioxygenase is a critical resistance mechanism in antitumor T cell immunotherapy targeting CTLA-4.
Holmgaard, R. B., et al. J Exp Med. 2013 Jul 1;210(7):1389-402. doi: 10.1084/jem.20130066. PMID: 23752227
This was also observed with antibodies targeting PD-1?PD-L1 and GITR. …Along these lines, we demonstrate that inhibition/absence of IDO in combination with therapies targeting immune checkpoints such as CTLA-4, PD-1/PD-L1, and GITR synergize to control tumor outgrowth and enhance overall survival in different tumor models. …In contrast to effector T cells, there was no significant increase in proliferation or up-regulation of PD-1 or ICOS by the tumor-infiltrating T reg cells with anti?CTLA-4 therapy. …Anti?PD-1/PD-L1 treatment delayed tumor progression in B16F10-bearing WT mice, but resulted in only modest improvement in survival. …However, anti?PD-1/PD-L1 treatment in IDO?/? mice resulted in significantly reduced tumor growth and improved survival.
Tags: bioactivity of Anti-Mouse PD-1 (CD279) [RMP1-14]; invivo anti-mouse PD-1 RMP1-14 of low endotoxin

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Q: Is there an Fc-silenced variant of the Anti-Mouse PD-1 (CD279) [RMP1-14] In Vivo Antibody available in Rabbit IgG?

Based on supplier data and product specifications, the Anti-Mouse PD-1 (CD279) [RMP1-14] In Vivo Antibody is available in Rabbit IgG format, but no Fc-silenced variants (e.g., with D265A or LALAPG mutations) are commercially offered in this isotype.

Standard Rabbit IgG Availability: The Anti-Mouse PD-1 (CD279) [RMP1-14] In Vivo Antibody in Rabbit IgG (e.g., chimeric or recombinant) is produced by suppliers like Syd Labs (catalog PA007162.rt) and Ushelf, with applications in IHC, FC, and in vivo assays, but without Fc-silencing mutations.
Fc-Silenced Alternatives: Fc-silenced versions of the Anti-Mouse PD-1 (CD279) [RMP1-14] In Vivo Antibody exist in mouse isotypes, such as Mouse IgG1-D265A (effectorless, from InvivoGen and Syd Labs) and Mouse IgG2a Fc Silent™, designed to eliminate ADCC/CDC for in vivo studies.
No Rabbit IgG Fc-Silenced Option: While reformatting services (e.g., from Absolute Antibody) allow custom engineering into Rabbit IgG, no pre-made Fc-silenced Rabbit IgG variant is listed, as silencing mutations are primarily applied to mouse Fc regions for reduced immunogenicity in murine models.

Q: How does the affinity and blocking ability of the Anti-Mouse PD-1 (CD279) [RMP1-14] In Vivo Antibody in Rabbit IgG compare to higher-affinity clones?

The Anti-Mouse PD-1 (CD279) [RMP1-14] In Vivo Antibody in Rabbit IgG format shares the variable region of the RMP1-14 clone, which is commonly compared to higher-affinity clones like 29F.1A12 and J43 in literature for PD-1/PD-L1 blockade in mouse models.

Affinity: The Anti-Mouse PD-1 (CD279) [RMP1-14] In Vivo Antibody binds mouse PD-1 with an apparent affinity of approximately 28.8 nM, which is ~100-fold weaker than the 0.42 nM affinity of clone 29F.1A12. Clone J43 shows intermediate affinity, generally between RMP1-14 and 29F.1A12, though quantitative data is less precise.
Blocking Ability: The Anti-Mouse PD-1 (CD279) [RMP1-14] In Vivo Antibody effectively blocks PD-1 interaction with PD-L1 and PD-L2, with IC50 values of 3.23 μg/mL for PD-L1 and 4.05 μg/mL for PD-L2, but it is ~100-fold less potent than 29F.1A12 (IC50 of 0.036 μg/mL for PD-L1 and 0.034 μg/mL for PD-L2). Compared to J43, RMP1-14 offers similar or slightly better blocking in some assays, but both are outperformed by 29F.1A12 in reversing T-cell inhibition. RMP1-14 also exhibits partial agonist activity at high PD-1 levels, unlike the pure blocking profile of 29F.1A12.

Overall, while the Anti-Mouse PD-1 (CD279) [RMP1-14] In Vivo Antibody in Rabbit IgG is suitable for PD-1 blockade, 29F.1A12 is superior in affinity and potency, serving as a better surrogate for high-affinity human PD-1 therapeutics. The Rabbit IgG format does not alter these intrinsic clone properties but may influence immunogenicity in vivo.

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Anti-Mouse PD-1 (CD279) [RMP1-14] In Vivo Antibody | PA007162.rt

Anti-Mouse PD-1 (CD279) [RMP1-14] In Vivo Antibody | PA007162.rt