Antibody Digestion to Fab or F(ab)2 Fragments
We use pepsin for F(ab')2 fragment production and papain for Fab fragment production.
We use pepsin for F(ab')2 fragment production and papain for Fab fragment production.
The most comprehensive custom rat polyclonal antibody development services: custom production of polyclonal antibodies against non-modified, phospho-specific, methylation-specific, and acetylation-specific peptides and proteins; polyclonal antibodies for ELISA, Western blot, IP, IF, ICC, and IHC applications....
Hybridoma antibody sequencing service(hybridoma sequencing, monoclonal antibody cloning, hybridoma cloning): all isotypes (IgG, IgM); antibody variable regions from mouse, rat, armenian hamster, rabbit, human; hybridomas or clonal B cells; sequenced after PCR amplification and DNA...
cheap custom rabbit polyclonal antibody production services to generate rabbit polyclonal antibodies (pAb) using peptides and proteins; custom rabbit polyclonal antibodies for ELISA, Western blot, and IHC applications. Guaranteed ELISA.
hybridoma-based custom mouse monoclonal antibody development services: production of anti-peptide, protein, chemical, cell monoclonal antibodies; DNA immunization; RIMMS; monoclonal antibodies for ELISA, Western blot, IHC, flow cytometry, diagnostics and therapeutics.
The most comprehensive rat custom monoclonal antibody development services: custom production of monoclonal antibodies against non-modified, phospho-specific, methylation-specific, and acetylation-specific peptides and proteins; custom monoclonal antibodies for ELISA, Western blot, IP, IF, ICC, and IHC...
Recombinant antibody (IgG) production services include: molecular construction of recombinant IgG antibodies; transient production of recombinant antibodies; purification of recombinant antibodies. Antibody isotype switching or class switching is available.
Recombinant antibody (scFv-Fc) production services include: molecular construction of recombinant scFv-Fc antibodies; transient production of recombinant antibodies; purification of recombinant antibodies.
Fast stable cell line generation service for antibody production: development of suspension CHO stable cell lines for antibody production. No virus involved. DNA Transfection and stable cell line screening only.
Kinetic analysis is used to determine the affinity of an antibody to its soluble antigen using surface plasmon resonance (SPR) or biolayer interferometry (BLI), which can measure kon, koff, and kD. Association and dissociation are...