Catalog No.	PA007144
Product Name	Rat IgG2b Isotype Control Antibody (12B9) | PA007144
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	Recombinant Rat IgG2b Isotype Control, Rat IgG2b Negative Control Antibody, rtIgG2b Isotype Control
Summary	The in vivo grade recombinant rat IgG2b isotype control antibody (rtIgG2b isotype control) was produced in mammalian cells.
Clone	12B9.
Isotype	rat IgG2b, kappa
Applications	an isotype-matched negative control for rat IgG2b kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Immunogen	N/A.
Form Of Antibody	0.2 μM filtered solution of 1x PBS.
Endotoxin	Less than 1 EU/mg of protein as determined by LAL method.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The recombinant Rat IgG2b Isotype Control Antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 1 month from date of receipt, 2 to 8°C as supplied. 3 months from date of receipt, -20°C to -70°C as supplied.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007144: Recombinant Rat IgG2b Isotype Control Antibody(12B9), In Vivo Grade

The Rat IgG2b Isotype Control Antibody (Clone 12B9) is an essential for researchers seeking to ensure the accuracy of immunological experiments. Its primary purpose is to serve as a negative control, helping to differentiate specific antibody binding from non-specific background signals in assays like flow cytometry, ELISA, immunohistochemistry (IHC), and in vivo studies. This recombinant antibody lacks reactivity to mouse, rat, or human proteins, making it ideal for validating the specificity of Rat IgG2b kappa primary antibodies. Researchers value its high purity (>95% by SDS-PAGE) and low endotoxin levels (<1 EU/mg), which are critical for reliable results in sensitive applications. Syd Labs’s in vivo grade recombinant rat IgG2b isotype control antibody (rtIgG2b isotype control) was produced in mammalian cells.

Customers often express concerns about consistency, versatility, and ease of use. The Rat IgG2b Isotype Control Antibody (12B9) addresses these by being recombinantly produced in CHO or HEK293 cells, ensuring lot-to-lot consistency and eliminating the variability associated with hybridoma-derived antibodies. It is available in multiple formats—purified, FITC, PE, APC conjugates, and Fc-silenced mutants—to suit diverse experimental needs, such as flow cytometry on mouse splenocytes or IHC on human tissues. The antibody is supplied as a 0.2 µm filtered solution in PBS (pH 7.4) or lyophilized with stabilizers, with clear instructions to store at 2-8°C for short-term use or -20°C for long-term stability, avoiding freeze-thaw cycles to maintain performance.

Please contact us to ask for a quote for the engineered rat IgG2b isotype control mutants, rtIgG2b isotype control antibody and mutants with Avi-, His-, and Flag-tags, and biotinylated rtIgG2b isotype control and mutants. A variety of conjugates (such as dyes and fluorophores) with the rat IgG2b isotype control antibody and Fc mutants are available.\

References of Rat IgG2b Isotype Control Antibody:

1、Reversal of indoleamine 2,3-dioxygenase-mediated cancer immune suppression by systemic kynurenine depletion with a therapeutic enzyme.
Triplett TA, et al. Nat Biotechnol. 2018 Sep;36(8):758-764. doi: 10.1038/nbt.4180. PMID: 30010674.
“Increased tryptophan (Trp) catabolism in the tumor microenvironment (TME) can mediate immune suppression by upregulation of interferon (IFN)-γ-inducible indoleamine 2,3-dioxygenase (IDO1) and/or ectopic expression of the predominantly liver-restricted enzyme tryptophan 2,3-dioxygenase (TDO). …Whether these effects are due to Trp depletion in the TME or mediated by the accumulation of the IDO1 and/or TDO (hereafter referred to as IDO1/TDO) product kynurenine (Kyn) remains controversial. …Here we show that administration of a pharmacologically optimized enzyme (PEGylated kynureninase; hereafter referred to as PEG-KYNase) that degrades Kyn into immunologically inert, nontoxic and readily cleared metabolites inhibits tumor growth. …Enzyme treatment was associated with a marked increase in the tumor infiltration and proliferation of polyfunctional CD8+ lymphocytes. …We show that PEG-KYNase administration had substantial therapeutic effects when combined with approved checkpoint inhibitors or with a cancer vaccine for the treatment of large B16-F10 melanoma, 4T1 breast carcinoma or CT26 colon carcinoma tumors.”

2、LAG3+ Regulatory T Cells Restrain Interleukin-23-Producing CX3CR1+ Gut-Resident Macrophages during Group 3 Innate Lymphoid Cell-Driven Colitis.
Bauché D, et al. Immunity. 2018 Aug 21;49(2):342-352.e5. doi: 10.1016/j.immuni.2018.07.007. PMID: 30097293.
“Rag2-/- were injected in i.p with either 500μg of anti-IL22 (RnD systems) at day -1 and day 2 post injection of anti-CD40 or 500μg of anti-LAG-3 (clone C9B7W, BioXCell), anti-ICOS (clone 7E.17G9,BioXCell) blocking antibodies or isotypes controls (rat IgG1, clone TN96A7 and Rat IgG2b, clone LTF2, BioXCell) 0,2 and 3 days prior anti-CD40 treatment. …Colon LP cells were isolated by first removing epithelial cells through the incubation of 0.5cm gut tissue pieces in Hank’s buffered salt solution containing 5mM EDTA and 10cmM HEPES for 20min at 37c°C and then repeating this incubation one additional time. …Cell suspensions were stained on ice for 30 min in the dark with various combinations of directly fluorochrome-conjugated antibodies. …LPS was added another 2hours after Treg cells and /myeloid co-culture. …Histology from colon strips were fixed in 10% neutral buffered formalin overnight, transferred to 70% ethanol, processed routinely, embedded in paraffin, sectioned at 4–5 μm, then stained with hematoxylin and eosin.”

3、Follicular regulatory T cells can be specific for the immunizing antigen and derive from naive T cells.
Aloulou M, et al. Nat Commun. 2016 Jan 28;7:10579. doi: 10.1038/ncomms10579. PMID: 26818004.
“For in vivo treatment, intravenous injections of 100 μg 10F.9G2 monoclonal antibody (anti-PD-L1, BioXcell, SKU: BE0101) or Rat IgG2b isotype control (SKU: BE0090) were performed at days 0 and +2 post immunization. …Differences between variables were evaluated using the non-parametric Mann–Whitney test. …All statistical analyses were carried out with the Prism 4.0 software (GraphPad). P values less than 0.05 were considered statistically significant. …Despite an increased interest in the role of Tfr cells in humoral immunity, many fundamental aspects of their biology remain unknown, including whether they recognize self- or foreign antigen. …We show that, in addition to developing from thymic derived Treg cells, Tfr cells can also arise from Foxp3− precursors in a PD-L1-dependent manner, if the adjuvant used is one that supports T-cell plasticity.”

4、PD-1 upregulated on regulatory T cells during chronic virus infection enhances the suppression of CD8+ T cell immune response via the interaction with PD-L1 expressed on CD8+ T cells.
Park HJ, et al. J Immunol. 2015 Jun 15;194(12):5801-11. doi: 10.4049/jimmunol.1401936. PMID: 25934860.
“Regulatory T (Treg) cells act as terminators of T cell immuniy during acute phase of viral infection; however, their role and suppressive mechanism in chronic viral infection are not completely understood. …In this study, we compared the phenotype and function of Treg cells during acute or chronic infection with lymphocytic choriomeningitis virus. …A contact between Treg and CD8+ T cells was necessary for the potent suppression of CD8+ T cell immune response. …More importantly, the suppression required cell-specific expression and interaction of PD-1 on chronic Treg cells and PD-1 ligand on CD8+ T cells. …Our study defines PD-1 upregulated on Treg cells and its interaction with PD-1 ligand on effector T cells as one cause for the potent T cell suppression and proposes the role of PD-1 on Treg cells, in addition to that on exhausted T cells, during chronic viral infection. …Chronic infection, unlike acute infection, led to a large expansion of Treg cells and their upregulation of programmed death-1 (PD-1).”

5、Radiation and dual checkpoint blockade activate non-redundant immune mechanisms in cancer.
Twyman-Saint Victor C, et al. Nature. 2015 Apr 16;520(7547):373-7. doi: 10.1038/nature14292. PMID: 25754329.
“This raises fundamental questions about mechanisms of non-redundancy and resistance. …Here we report major tumour regressions in a subset of patients with metastatic melanoma treated with an anti-CTLA4 antibody (anti-CTLA4) and radiation, and reproduced this effect in mouse models. …Accordingly, optimal response in melanoma and other cancer types requires radiation, anti-CTLA4 and anti-PD-L1/PD-1. Anti-CTLA4 predominantly inhibits T-regulatory cells (Treg cells), thereby increasing the CD8 T-cell to Treg (CD8/Treg) ratio. …Addition of PD-L1 blockade reverses T-cell exhaustion to mitigate depression in the CD8/Treg ratio and further encourages oligoclonal T-cell expansion. …Similarly to results from mice, patients on our clinical trial with melanoma showing high PD-L1 did not respond to radiation plus anti-CTLA4, demonstrated persistent T-cell exhaustion, and rapidly progressed.”

6、Micro-RNA-155-mediated control of heme oxygenase 1 (HO-1) is required for restoring adaptively tolerant CD4+ T-cell function in rodents.
Vandevenne, P., et al. Eur J Immunol. 2015 Mar;45(3):829-42. doi: 10.1002/eji.201445066. PMID: 25641586.
“Injections of isotype control IgG2b (Clone LTF-2; BioXcell) were also conducted. In all experiments, mice were sacrificed at day 21 for analysis. …Purified T cells were stimulated with plate-bound anti-CD3 antibody and soluble anti-CD28 antibody (BD Biosciences). …Lentiviral vectors containing shHmox1 and control vector were purchased from Thermo Scientific. …Mice were sacrificed and organs were frozen and mounted in embedding medium (Tissue-Tek). …Sections were observed on a Nikon Eclipse 80i microscope and recorded by Nikon Digital Camera DXM1200F.”

7、Ectopic lymphoid structures function as microniches for tumor progenitor cells in hepatocellular carcinoma.
Finkin S, et al. Nat Immunol. 2015 Dec;16(12):1235-44. doi: 10.1038/ni.3290. PMID: 26502405.
“Ectopic lymphoid-like structures (ELSs) are often observed in cancer, yet their function is obscure. …Although ELSs signify good prognosis in certain malignancies, we found that hepatic ELSs indicated poor prognosis for hepatocellular carcinoma (HCC). …We studied an HCC mouse model that displayed abundant ELSs and found that they constituted immunopathological microniches wherein malignant hepatocyte progenitor cells appeared and thrived in a complex cellular and cytokine milieu until gaining self-sufficiency. …The egress of progenitor cells and tumor formation were associated with the autocrine production of cytokines previously provided by the niche. …Such aberrant immunological foci might represent new targets for cancer therapy.”

8、Role of peripheral immune response in microglia activation and regulation of brain chemokine and proinflammatory cytokine responses induced during VSV encephalitis.
Steel CD, et al. J Neuroimmunol. 2014 Feb 15;267(1-2):50-60. doi: 10.1016/j.jneuroim.2013.12.002. PMID: 24369299.
“We report herein that neuroinvasion by vesicular stomatitis virus (VSV) activates microglia and induces a peripheral dendritic cell (DC)-dependent inflammatory response in the central nervous system (CNS). …VSV neuroinvasion rapidly induces multiple brain chemokine and proinflammatory cytokine mRNAs that display bimodal kinetics. …Peripheral DC ablation or T cell depletion suppresses the second wave of this response demonstrating that infiltrating T cells are primarily responsible for the bimodal characteristics of this response. …The robust infiltrate associated with VSV encephalitis likely depends on sustained production of brain CCL19 and CCR7 expression on infiltrating inflammatory cells.”

9、Programmed death-1 impairs secondary effector lung CD8⁺ T cells during respiratory virus reinfection.
Erickson JJ, et al. J Immunol. 2014 Nov 15;193(10):5108-17. doi: 10.4049/jimmunol.1302208. PMID: 25339663.
“Reinfections with respiratory viruses are common and cause significant clinical illness, yet precise mechanisms governing this susceptibility are ill defined. …Lung Ag-specific CD8+ T cells (TCD8) are impaired during acute viral lower respiratory infection by the inhibitory receptor programmed death-1 (PD-1). …To determine whether PD-1 contributes to recurrent infection, we first established a model of reinfection by challenging B cell–deficient mice with human metapneumovirus (HMPV) several weeks after primary infection, and found that HMPV replicated to high titers in the lungs. …In vitro blockade demonstrated that PD-1 was the dominant inhibitory receptor early after reinfection. …These results have important implications for the design of effective vaccines against respiratory viruses.”

10、Highly pathological influenza A virus infection is associated with augmented expression of PD-1 by functionally compromised virus-specific CD8+ T cells.
Sharma S, et al. J Virol. 2014 Feb;88(3):1636-51. doi: 10.1128/JVI.02851-13. PMID: 24257598.
“Epithelial cells at the site of influenza virus infection in the airway microenvironment initiate an early and rapid cascade of cytokine production, followed by local inflammation and ensuing responses. …Although highly pathogenic H5N1 avian influenza viruses have been unable to efficiently cross over to the human population thus far, mortality continues to hover around 60%. …It has been established that initiation of the adaptive response is heavily influenced by innate immunity. …With time, as demonstrated in chronic infections, CD8+ T cells can become exhausted and lose their functionality. …However, that work used strains of Listeria monocytogenes that expressed an epitope from the influenza virus hemagglutinin protein rather than actual influenza virus infection.”

For more references about Rat IgG2b Isotype Control Antibody please contact our scientific support team with message@sydlabs.com.

Other in vivo grade Recombinant IgG Isotype Control Antibodies and Mutants:
Recombinant Rat IgG1 Isotype Control Antibody, In vivo Grade
Recombinant Rat IgG2a Isotype Control Antibody, In vivo Grade
Recombinant Rat IgG2b Isotype Control Antibody, In vivo Grade
Recombinant Rat IgG2c Isotype Control Antibody, In vivo Grade
Recombinant Human IgG1 Isotype Control Antibody and Mutants, In vivo Grade
Recombinant Human IgG2 Isotype Control Antibody, In vivo Grade
Recombinant Human IgG3 Isotype Control Antibody, In vivo Grade
Recombinant Human IgG4-S228P Isotype Control Antibody and Mutants, In vivo Grade
Recombinant Mouse IgG1 Isotype Control Antibody and Mutants, In vivo Grade
Recombinant Mouse IgG2a Isotype Control Antibody and Mutants, In vivo Grade
Recombinant Mouse IgG2b Isotype Control Antibody and Mutants, In vivo Grade
Recombinant Mouse IgG2c Isotype Control Antibody and Mutants, In vivo Grade
Recombinant Mouse IgG3 Isotype Control Antibody, In vivo Grade
Recombinant Hamster IgG1 Isotype Control Antibody, In vivo Grade
Recombinant Hamster IgG2 Isotype Control Antibody, In vivo Grade

In vivo Grade Recombinant IgG Fc Proteins:
Recombinant Rat IgG2a Fc Protein (rtIgG2a), In vivo Grade
Recombinant Rat IgG2b Fc Protein (rtIgG2b), In vivo Grade
Recombinant Human IgG1 Fc Protein (hIgG1), In vivo Grade
Recombinant Human IgG2 Fc Protein (hIgG2), In vivo Grade
Recombinant Human IgG4 Fc Protein (hIgG4), In vivo Grade
Recombinant Mouse IgG1 Fc Protein (mIgG1), In vivo Grade
Recombinant Mouse IgG2a Fc Protein (mIgG2a), In vivo Grade
Recombinant Mouse IgG2b Fc Protein (mIgG2b), In vivo Grade
Recombinant Llama IgG2b Fc Protein (lIgG2b), In vivo Grade
Recombinant Rabbit IgG Fc Protein (rIgG), In vivo Grade

Rat IgG2b Isotype Control(12B9) from: Rat IgG2b Isotype Control Antibody: PA007144 Syd Labs

No more offers for this product!