Biotinylated Anti-mouse PD1 Antibody (RMP1-14.1, Human IgG1 Fc Silent Kappa)

Catalog No.	PA007733.h1Fs-b
Product Name	Biotinylated Recombinant Anti-mouse PD1 Monoclonal Antibody (Clone: RMP1-14.1), Human IgG1 Fc Silent Kappa
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Clone	RMP1-14.1
Isotype	Human IgG1 Fc Silent Kappa
Source/Host	The site-directed biotinylated anti-mouse PD1 monoclonal antibody (clone: RMP1-14.1) was produced in mammalian cells.
Specificity/Sensitivity	The site-directed biotinylated recombinant RMP1-14.1 antibody specifically binds to mouse PD1 protein.
Applications	Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form Of Antibody	0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Conjugate	Biotin-conjugated
Purity	>95% by SDS-PAGE under reducing conditions and HPLC
Shipping	The RMP1-14.1 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant rat IgG2a isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007733.h1Fs-b: Biotinylated Recombinant Anti-mouse PD1 Monoclonal Antibody (Clone: RMP1-14.1), Human IgG1 Fc Silent Kappa

Recombinant human IgG1 monoclonal antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.

The rat anti-mouse PD-1 monoclonal antibody (clone No. RMP1-14, rat IgG2a kappa) reacts with the mouse PD-1 protein (CD279 or programmed death-1) encoded by the mouse pdcd1 gene, a member of the CD28 family of the Ig superfamily. PD-1 has two ligands, PD-L1 and PD-L2, both of which belong to the B7 family. It has been shown that in mouse models of melanoma, tumor growth can be transiently arrested via treatment with the anti-mouse PD 1 antibody and the anti-mouse PD L1 antibody which block the interaction between the PD-L1 protein and its receptor PD-1 protein. The RMP1-14 antibody blocks the binding of both the mouse PD-L1 protein and the mouse PD-L2 protein to the mouse PD-1 protein.
Our recombinant RMP1-14 antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse PD-1 antibody (hybridoma clone name or number: RMP1-14). The murinized anti-mouse PD-1 recombinant antibody has not only the constant regions of the mouse IgG antibodies but also the murinized variable regions.

References of Anti-Mouse PD-1 Monoclonal Antibody (Clone: RMP1-14):
1. A Comparison of Murine PD-1 and PD-L1 Monoclonal Antibodies

Bu, M. T., et al. Monoclon Antib Immunodiagn Immunother. 2022 Aug;41(4):202-209. PMID: 35925787

“Unconjugated and fluorophore-conjugated PD-1 mAbs (1A12, rat IgG2a; RMP1-14, rat IgG2a; and RMP1-30, rat IgG2b) and PD-L1 mAbs (10F.9G2, rat IgG2b; MIH6, rat IgG2a) were purchased from…Owing to the widespread use of both PD-1 mAbs 1A12 and RMP1-14 in experimental mouse models, we compared the avidities of these two mAbs. …The 1A12 antimouse PD-1 mAb bound to mPD-1-transfected Jurkat cells in a dose-dependent manner with an apparent affinity of 0.42 nM, while RMP1-14 antibody bound with a much weaker apparent affinity of 28.8 nM.”

2. Dying cells expose a nuclear antigen cross-reacting with anti-PD-1 monoclonal antibodies

Metzger, P., et al. Sci Rep. 2018 Jun 11;8(1):8810. PMID: 29892077

“We assessed PD-1 expression by flow cytometry and compared the two mainly used antibody clones (29 F.1A12 and RMP1-14 antibodies), which were also included in the above-mentioned study. …We observed PD-1 staining exclusively in the dead cell fraction for both clones 29 F.1A12 and RMP1-14 mAb, respectively…”

3. The Antitumor Activity of Combinations of Cytotoxic Chemotherapy and Immune Checkpoint Inhibitors Is Model-Dependent

Grasselly, C., et al. Front Immunol. 2018 Oct 9;9:2100. PMID: 30356816

“Anti-PD1 antibody (clone RMP1-14) or anti-PDL1 (clone 10F.9G2) mAb were administered in combination with chemotherapy regimens in SC bladder cancer MB49 and MBT-2, metastatic breast cancer 4T1, and colorectal cancer MC38.”

4. Type I MET inhibitors cooperate with PD-1 blockade to promote rejection of hepatocellular carcinoma

DeAzevedo, R., et al. J Immunother Cancer. 2024 Oct 30;12(10):e009690. PMID: 39477243

“Mice received anti-PD-1 antibody RMP1-14 i.p. or isotype control antibody to evaluate cooperation with Type I MET inhibitors in promoting rejection of hepatocellular carcinoma.”

5. Impact of mouse model tumor implantation site on acquired resistance to anti-PD-1 immune checkpoint therapy

Denis, M., et al. Front Immunol. 2023 Jan 10;13:1011943. PMID: 36703964

“When the tumor volume reached 150 mm3, mice were randomized and received first treatment of anti-PD-1 antibody (RMP1-14, 12.5 mg/kg per week, intraperitoneal (IP)) to evaluate the impact of tumor implantation site on acquired resistance.”

For more technical references or data sheets regarding the Recombinant Anti-Mouse PD-L1 Monoclonal Antibody (Clone: 10F.9G2) formats, please contact our scientific support team at message@sydlabs.com.

Syd Labs provides the following in vivo grade recombinant anti-mouse PD-L1 / PD-1 monoclonal antibodies:
In vivo grade recombinant anti-mouse PD1 monoclonal antibodies (Clone 29F.1A12)
In vivo grade recombinant anti-mouse PD-1 monoclonal antibodies (Clone RMP1-14)
In vivo grade recombinant anti-mouse PD-L1 monoclonal antibodies (Clone 10F.9G2)

Syd Labs provides the following in vivo grade recombinant anti-mouse PD-1 / PD-L1 bispecific antibodies:
In vivo grade recombinant anti-mouse PD-1 / PD-1 bispecific antibodies (Clone RMP1-14 / 29F.1A12)
In vivo grade recombinant anti-mouse PD-1 / PD-1 bispecific antibodies (Clone 29F.1A12 / RMP1-14)
In vivo grade recombinant anti-mouse PD-1 / PD-L1 bispecific antibodies (Clone RMP1-14 / 10F.9G2)
In vivo grade recombinant anti-mouse PD-L1 / PD-1 bispecific antibodies (Clone 10F.9G2 / RMP1-14)
In vivo grade recombinant anti-mouse PD-1 / PD-L1 bispecific antibodies (Clone 29F.1A12 / 10F.9G2)
In vivo grade recombinant anti-mouse PD-L1 / PD-1 bispecific antibodies (Clone 10F.9G2 / 29F.1A12)

Syd Labs provides the following recombinant anti-mouse PD-1 / PD-L1 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse PD-1 monoclonal antibodies (Clone: RMP1-14) for flow cytometry
Recombinant Anti-mouse PD-1 monoclonal antibodies (Clone: 29F.1A12) for flow cytometry
Recombinant Anti-mouse PD-L1 monoclonal antibodies (Clone: 10F.9G2) for flow cytometry

Recombinant PD1 and PDL1 Proteins:
Recombinant Human PD-1/PDCD1 Proteins
Biotinylated Recombinant Human PD-1/PDCD1 Proteins
Recombinant Mouse PD-1/PDCD1 Proteins
Biotinylated Recombinant Mouse PD-1/PDCD1 Proteins
Recombinant Cynomolgus PD-1/PDCD1 Proteins
Recombinant Canine PD-1/PDCD1 Proteins
Biotinylated Cynomolgus/Rhesus macaque PD-L1 Protein
Biotinylated Human PD-L1 Protein
Biotinylated Mouse PD-L1 Protein
Cynomolgus PD-L1 Protein
Cynomolgus/Rhesus macaque PD-L1 Protein
Human PD-L1/B7-H1 Protein
Mouse PD-L1/B7-H1 Protein