Biotinylated Anti-mouse PD1 Antibody (29F.1A12.1, Human IgG1 Fc Silent Kappa)

Catalog No.	PA007734.h1Fs-b
Product Name	Biotinylated Recombinant Anti-mouse PD1 Monoclonal Antibody (Clone: 29F.1A12.1), Human IgG1 Fc Silent Kappa
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Clone	29F.1A12.1
Isotype	Human IgG1 Fc Silent Kappa
Source/Host	The site-directed biotinylated anti-mouse PD1 monoclonal antibody (clone: 29F.1A12.1) was produced in mammalian cells.
Specificity/Sensitivity	The site-directed biotinylated recombinant 29F.1A12.1 antibody specifically binds to mouse PD1 protein.
Applications	Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form Of Antibody	0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Conjugate	Biotin-conjugated
Purity	>95% by SDS-PAGE under reducing conditions and HPLC
Shipping	The 29F.1A12.1 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant rat IgG2b isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007734.h1Fs-b: Biotinylated Recombinant Anti-mouse PD1 Monoclonal Antibody (Clone: 29F.1A12.1), Human IgG1 Fc Silent Kappa

Recombinant human IgG1 monoclonal antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.

The rat anti-mouse PD-1 monoclonal antibody 29F.1A12 (rat IgG2a kappa) reacts with the mouse PD-1 protein (programmed death-1 or CD279) encoded by the mouse Pdcd1 gene, a member of the CD28 family of the Ig superfamily. PD-1 has two ligands, PD-L1 and PD-L2, both of which belong to the B7 family. It has been shown that in mouse models of melanoma, tumor growth can be transiently arrested via treatment with the anti-mouse PD-1 and anti-mouse PD-L1 antibodies which block the interaction between the PD-L1 protein and its receptor PD-1 protein. The 29F.1A12 monoclonal antibody blocks the binding of both the mouse PD-L1 protein and the mouse PD-L2 protein to the mouse PD-1 protein.
Our recombinant 29F.1A12 antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse PD-1 monoclonal antibody (hybridoma clone name or number: 29F.1A12).

References of Anti-Mouse PD-1 Monoclonal Antibody (Clone: 29F.1A12):
1. Anti-PD-1 therapy triggers Tfh cell-dependent IL-4 release to boost CD8 T cell responses in tumor-draining lymph nodes

Ruggiu, M., et al. J Exp Med. 2024;221(4):e20232104. PMID: 38417020

“Lymph node cells were stained ex vivo with a different anti-PD-1 mAb clone that did not interfere with the injected mAb to link in vivo labeling with PD-1 surface expression levels. …Representative FACS dot plot showing in vivo labeling by AF594-labeled anti-PD-1 mAb (clone RMP1-14) and ex vivo staining by anti-PD-1 antibody (clone 29F.1A12). …anti-mouse PD1-BX036 (purified clone 29F.1A12).”

2. Distinct antibody clones detect PD-1 checkpoint expression and block PD-L1 interactions on live murine melanoma cells

Martins, C., et al. Sci Rep. 2022;12(1):12491. PMID: 35864188

“Similarly, in preclinical cancer models, several PD-1 blocking ab clones, including 29F.1A12 (anti-mouse PD-1 antibody), thwart tumor progression. …For example, TME-infiltrating macrophages and natural killer (NK) cells both express PD-1, the blockade of which by 29F.1A12 (anti-mouse PD-1 mAb) contributes to tumor growth suppression and prolonged survival. …29F.1A12 antibody recognizes PD-1 on B16-F10 and other murine tumor lines, suppresses cancer cell-intrinsic PD-1 receptor signaling, and inhibits resultant tumor growth in three-dimensional (3D) culture and tumor-bearing mice.”

3. Dying cells expose a nuclear antigen cross-reacting with anti-PD-1 monoclonal antibodies

Metzger, P., et al. Sci Rep. 2018;8(1):9040. PMID: 29892077

“In addition, PD-1 staining (clone 29F1A12) uncovered a FSC-H low population predominantly positive for PD-1. …PD-1 (clone 29F.1A12) and fixable viability dye (FVD) staining was assessed by flow cytometry. …PD-1 staining (clone 29F.1A12) in these cells could be due to binding of an intracellular PD-1 pool that becomes accessible in cells with leaky membranes. …Again, PD-1 mAb staining (clone 29F.1A12) was found in both PD-1 wild-type and knock-out cells in the dead cell fraction, indicative of unspecific binding. …We assessed PD-1 expression by flow cytometry and compared the two mainly used antibody clones (29F.1A12 and RMP1-14), which were also included in the above-mentioned study.”

4. Adaptive resistance to therapeutic PD-1 blockade is associated with upregulation of alternative immune checkpoints

Koyama, S., et al. Nat Commun. 2016;7:10501. PMID: 26883990

“Fluorescent conjugated anti-PD-1 antibody is the same clone (29F.1A12) as the therapeutic antibody. …PD-1-blocking antibody (clone 29F.1A12), TIM-3-blocking antibody (clone RMT3-23) and their isotype controls (clone 2A3) were injected intraperitoneally into the mice for therapeutic treatment (three times a week, 200 μg for PD1 and 100 μg for TIM-3 per dose).”

5. The PD-1 Pathway Regulates Development and Function of Memory CD8+ T Cells following Respiratory Viral Infection

Pauken, K. E., et al. Cell Rep. 2020;31(13):107827. PMID: 32610128

“WT B6 mice were treated with anti-PD-1 antibody (clone 29F.1A12) or isotype control antibody (rat IgG2a) on days -1, 2, and 5 and harvested on day 8 p.i., or treated on days -1, 2, 5, and 8 and harvested on day 60+ p.i. PD-L1/L2 DKO mice were included as a control. …For in vivo antibody blockade experiments, WT C57BL/6 mice were given 200 μg per mouse per injection of rat anti-PD-1 antibody (clone 29F.1A12) or isotype control antibody (Rat IgG2a, clone 2A3) on days -1, 2, and 5 p.i. and analyzed at day 8 p.i., or on days -1, 2, 5, and 8 p.i. and analyzed at day 60+ p.i.”

For more technical references or data sheets regarding the Recombinant Anti-Mouse PD-L1 Monoclonal Antibody (Clone: 10F.9G2) formats, please contact our scientific support team at message@sydlabs.com.

Syd Labs provides the following in vivo grade recombinant anti-mouse PD-L1 / PD-1 monoclonal antibodies:
In vivo grade recombinant anti-mouse PD1 monoclonal antibodies (Clone 29F.1A12)
In vivo grade recombinant anti-mouse PD-1 monoclonal antibodies (Clone RMP1-14)
In vivo grade recombinant anti-mouse PD-L1 monoclonal antibodies (Clone 10F.9G2)

Syd Labs provides the following in vivo grade recombinant anti-mouse PD-1 / PD-L1 bispecific antibodies:
In vivo grade recombinant anti-mouse PD-1 / PD-1 bispecific antibodies (Clone RMP1-14 / 29F.1A12)
In vivo grade recombinant anti-mouse PD-1 / PD-1 bispecific antibodies (Clone 29F.1A12 / RMP1-14)
In vivo grade recombinant anti-mouse PD-1 / PD-L1 bispecific antibodies (Clone RMP1-14 / 10F.9G2)
In vivo grade recombinant anti-mouse PD-L1 / PD-1 bispecific antibodies (Clone 10F.9G2 / RMP1-14)
In vivo grade recombinant anti-mouse PD-1 / PD-L1 bispecific antibodies (Clone 29F.1A12 / 10F.9G2)
In vivo grade recombinant anti-mouse PD-L1 / PD-1 bispecific antibodies (Clone 10F.9G2 / 29F.1A12)

Syd Labs provides the following recombinant anti-mouse PD-1 / PD-L1 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse PD-1 monoclonal antibodies (Clone: RMP1-14) for flow cytometry
Recombinant Anti-mouse PD-1 monoclonal antibodies (Clone: 29F.1A12) for flow cytometry
Recombinant Anti-mouse PD-L1 monoclonal antibodies (Clone: 10F.9G2) for flow cytometry

Recombinant PD1 and PDL1 Proteins:
Recombinant Human PD-1/PDCD1 Proteins
Biotinylated Recombinant Human PD-1/PDCD1 Proteins
Recombinant Mouse PD-1/PDCD1 Proteins
Biotinylated Recombinant Mouse PD-1/PDCD1 Proteins
Recombinant Cynomolgus PD-1/PDCD1 Proteins
Recombinant Canine PD-1/PDCD1 Proteins
Biotinylated Cynomolgus/Rhesus macaque PD-L1 Protein
Biotinylated Human PD-L1 Protein
Biotinylated Mouse PD-L1 Protein
Cynomolgus PD-L1 Protein
Cynomolgus/Rhesus macaque PD-L1 Protein
Human PD-L1/B7-H1 Protein
Mouse PD-L1/B7-H1 Protein