Biotinylated Anti-mouse PD-L1 Antibody (10F.9G2.1, Human IgG1 Fc Silent Kappa)

Catalog No.	PA007735.h1Fs-b
Product Name	Biotinylated Recombinant Anti-mouse PD-L1 Monoclonal Antibody (Clone: 10F.9G2.1), Human IgG1 Fc Silent Kappa
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Clone	10F.9G2.1.
Isotype	Human IgG1 Fc Silent Kappa
Source/Host	The site-directed biotinylated anti-mouse PD-L1 monoclonal antibody (clone: 10F.9G2.1) was produced in mammalian cells
Specificity/Sensitivity	The site-directed biotinylated recombinant 10F.9G2.1 antibody specifically binds to mouse PD-L1 protein
Applications	Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form Of Antibody	0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Conjugate	Biotin-conjugated.
Purity	>95% by SDS-PAGE under reducing conditions and HPLC
Shipping	The 10F.9G2.1 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant rat IgG2b isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007735.h1Fs-b: Biotinylated Recombinant Anti-mouse PD-L1 Monoclonal Antibody (Clone: 10F.9G2.1), Human IgG1 Fc Silent Kappa

Recombinant human IgG1 monoclonal antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.

The rat anti-mouse PD-L1 monoclonal antibody 10F.9G2 (rat IgG2b kappa) reacts with the mouse PD-L1 protein (programmed death ligand-1, B7-H1 or CD274), a member of the B7 family of the Ig superfamily. PD-1 has two ligands, PD-L1 and PD-L2. It has been shown that in mouse models of melanoma, tumor growth can be transiently arrested via treatment with the anti-mouse PD-1 and anti-mouse PD-L1 antibodies which block the interaction between the PD-L1 protein and its receptor PD-1 protein. The 10F.9G2 monoclonal antibody blocks the binding of the mouse PD-L1 protein to the mouse PD-1 protein.
Our recombinant 10F.9G2 antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse PD L1 monoclonal antibody (hybridoma clone name or number: 10F.9G2).

References of Anti-Mouse PD-L1 Monoclonal Antibody (Clone: 10F.9G2):
1. Antibody targeting tumor-derived soluble NKG2D ligand sMIC provides dual co-stimulation of CD8 T cells and enables sMIC+ tumors respond to PD1/PD-L1 blockade therapy

Zhang J, et al. J Immunother Cancer. 2019 Aug 23;7(1):223. PMID: 31446896

“TRAMP/MICB male mice of 26 to 28 week-old were assigned into four cohorts with similar distribution of serum sMIC receiving therapy with intraperitoneal (i.p.) injection of sMIC-targeting nonblocking monoclonal antibody B10G5 or anti-PD-L1 (clone 10F.9G2, BioXCell) antibody or isotype control IgG (cIgG) respectively at a respective dose of 4.0 mg/kg body weight twice weekly. Combination of the following antibody was used for cell surface or intracellular staining to define populations of NK, CD8, and subsets of CD4 T cells: CD3e (clone 145-2c11), CD8a (clone 53-6.7), CD4 (clone GK1.5), NK1.1 (clone PK136), NKG2D (clone CX5), CD44 (clone eBio4B10), CD11c (clone N418), MHCII (clone M5/114.15.2), CD80 (clone 16-10A1), CD86 (clone PO3) and CD40 (clone 1C10).”

For more technical references or data sheets regarding the Recombinant Anti-Mouse PD-L1 Monoclonal Antibody (Clone: 10F.9G2) formats, please contact our scientific support team at message@sydlabs.com.

Syd Labs provides the following in vivo grade recombinant anti-mouse PD-L1 / PD-1 monoclonal antibodies:
In vivo grade recombinant anti-mouse PD1 monoclonal antibodies (Clone 29F.1A12)
In vivo grade recombinant anti-mouse PD-1 monoclonal antibodies (Clone RMP1-14)
In vivo grade recombinant anti-mouse PD-L1 monoclonal antibodies (Clone 10F.9G2)

Syd Labs provides the following in vivo grade recombinant anti-mouse PD-1 / PD-L1 bispecific antibodies:
In vivo grade recombinant anti-mouse PD-1 / PD-1 bispecific antibodies (Clone RMP1-14 / 29F.1A12)
In vivo grade recombinant anti-mouse PD-1 / PD-1 bispecific antibodies (Clone 29F.1A12 / RMP1-14)
In vivo grade recombinant anti-mouse PD-1 / PD-L1 bispecific antibodies (Clone RMP1-14 / 10F.9G2)
In vivo grade recombinant anti-mouse PD-L1 / PD-1 bispecific antibodies (Clone 10F.9G2 / RMP1-14)
In vivo grade recombinant anti-mouse PD-1 / PD-L1 bispecific antibodies (Clone 29F.1A12 / 10F.9G2)
In vivo grade recombinant anti-mouse PD-L1 / PD-1 bispecific antibodies (Clone 10F.9G2 / 29F.1A12)

Syd Labs provides the following recombinant anti-mouse PD-1 / PD-L1 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse PD-1 monoclonal antibodies (Clone: RMP1-14) for flow cytometry
Recombinant Anti-mouse PD-1 monoclonal antibodies (Clone: 29F.1A12) for flow cytometry
Recombinant Anti-mouse PD-L1 monoclonal antibodies (Clone: 10F.9G2) for flow cytometry

Recombinant PD1 and PDL1 Proteins:
Recombinant Human PD-1/PDCD1 Proteins
Biotinylated Recombinant Human PD-1/PDCD1 Proteins
Recombinant Mouse PD-1/PDCD1 Proteins
Biotinylated Recombinant Mouse PD-1/PDCD1 Proteins
Recombinant Cynomolgus PD-1/PDCD1 Proteins
Recombinant Canine PD-1/PDCD1 Proteins
Biotinylated Cynomolgus/Rhesus macaque PD-L1 Protein
Biotinylated Human PD-L1 Protein
Biotinylated Mouse PD-L1 Protein
Cynomolgus PD-L1 Protein
Cynomolgus/Rhesus macaque PD-L1 Protein
Human PD-L1/B7-H1 Protein
Mouse PD-L1/B7-H1 Protein