Recombinant Anti-mouse PD-L1 Monoclonal Antibody (Clone: 10F.9G2.1), Rat IgG2b Kappa PA007506.r2b Syd Labs

Anti-mouse PD-L1 Antibody for Flow Cytometry | PA007506.r2b

$55.00 – $110.00

Recombinant rat IgG2b isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.

Details & Specifications
References

Catalog No.	PA007506.r2b
Product Name	Recombinant Anti-mouse PD-L1 Monoclonal Antibody (Clone: 10F.9G2.1), Rat IgG2b Kappa
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	Programmed Cell Death Protein 1, PD-1, CD279, Cluster of Differentiation 279, RMP1-14
Clone	10F.9G2.1.
Isotype	Rat IgG2b Kappa.
Source/Host	The anti-mouse PD-L1 monoclonal antibody (clone: 10F.9G2.1) for Flow Cytometry was produced in mammalian cells.
Specificity/Sensitivity	The recombinant 10F.9G2.1 antibody for Flow Cytometry specifically binds to mouse PD-L1 protein.
Applications	Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form Of Antibody	0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Conjugate	Non-conjugated.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The Recombinant Anti-mouse PD-L1 Monoclonal Antibody (Clone: 10F.9G2.1) for Flow Cytometry, Rat IgG2b Kappa is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
Note	Recombinant rat IgG2b isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007506.r2b: Recombinant Anti-mouse PD-L1 Monoclonal Antibody (Clone: 10F.9G2.1) for Flow Cytometry, Rat IgG2b Kappa, In vivo Grade

The rat anti-mouse PD-L1 monoclonal antibody 10F.9G2 (rat IgG2b kappa) reacts with the mouse PD-L1 protein (programmed death ligand-1, B7-H1 or CD274), a member of the B7 family of the Ig superfamily. PD-1 has two ligands, PD-L1 and PD-L2. It has been shown that in mouse models of melanoma, tumor growth can be transiently arrested via treatment with the anti-mouse PD-1 and anti-mouse PD-L1 antibodies which block the interaction between the PD-L1 protein and its receptor PD-1 protein. The 10F.9G2 monoclonal antibody blocks the binding of the mouse PD-L1 protein to the mouse PD-1 protein.
Our recombinant 10F.9G2 antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse PD L1 monoclonal antibody (hybridoma clone name or number: 10F.9G2).

References of Anti-Mouse PD-L1 Monoclonal Antibody (Clone: 10F.9G2)

1. Antibody targeting tumor-derived soluble NKG2D ligand sMIC provides dual co-stimulation of CD8 T cells and enables sMIC+ tumors respond to PD1/PD-L1 blockade therapy.
Zhang J, et al.J Immunother Cancer. 2019 Aug 23;7(1):223. doi: 10.1186/s40425-019-0693-y. PMID: 31446896
“TRAMP/MICB male mice of 26 to 28 week-old were assigned into four cohorts with similar distribution of serum sMIC (Additional file 1: Figure S1) receiving therapy with intraperitoneal (i.p.) injection of sMIC-targeting nonblocking monoclonal antibody B10G5 [28] or anti-PD-L1 (clone 10F.9G2, BioXCell) antibody or isotype control IgG (cIgG) respectively at a respective dose of 4.0 mg/kg body weight twice weekly. Combination of the following antibody was used for cell surface or intracellular staining to define populations of NK, CD8, and subsets of CD4 T cells: CD3e (clone 145-2c11), CD8a (clone 53-6.7), CD4 (clone GK1.5), NK1.1 (clone PK136), NKG2D (clone CX5), CD44 (clone eBio4B10), CD11c (clone N418), MHCII (clone M5/114.15.2), CD80 (clone 16-10A1), CD86 (clone PO3) and CD40 (clone 1C10). Multicolored Flow cytometry analyses were performed on an LSR II (BD). Representative histograms from flow cytometry analyses demonstrating the percentage of PD-L1+ tumor cells from TRAMP/MICB mice. Representative histograms from flow cytometry analyses of CD40, CD80 and CD86 expression on DCs from tumor-draining lymph nodes and tumor beds.”

For more technical references or data sheets regarding the Recombinant Anti-Mouse PD-L1 Monoclonal Antibody (Clone: 10F.9G2) formats, please contact our scientific support team at message@sydlabs.com.

Syd Labs provides the following anti-mouse PD-L1 / PD-1 antibodies:

recombinant anti-mouse PD1 monoclonal antibodies (Clone 29F.1A12.1), In vivo Grade
recombinant anti-mouse PD-1 monoclonal antibodies (Clone RMP1-14.1), In vivo Grade
recombinant anti-mouse PD-L1 monoclonal antibodies (Clone 10F.9G2.1), In vivo Grade

Recombinant PD1 and PDL1 Proteins:

Recombinant Human PD-1/PDCD1 Proteins
Biotinylated Recombinant Human PD-1/PDCD1 Proteins
Recombinant Mouse PD-1/PDCD1 Proteins
Biotinylated Recombinant Mouse PD-1/PDCD1 Proteins
Recombinant Cynomolgus PD-1/PDCD1 Proteins
Recombinant Canine PD-1/PDCD1 Proteins
Biotinylated Cynomolgus/Rhesus macaque PD-L1 Protein
Biotinylated Human PD-L1 Protein
Biotinylated Mouse PD-L1 Protein
Cynomolgus PD-L1 Protein
Cynomolgus/Rhesus macaque PD-L1 Protein
Human PD-L1/B7-H1 Protein
Mouse PD-L1/B7-H1 Protein