Extensive experience with making DNA constructs for albumin fusion protein production, expressing and purifying a number of albumin fusion proteins from microgram to kilogram scales in mammalian cells, such as CHO and HEK 293 cells.
Gene synthesis service. Synthesize any DNA sequence of your interest. We may synthesize codon-optimized cDNA, gene variants, artificially designed DNA, or any other sequence for your research.
Crude and purified peptide library synthesis services. Overlapping, truncation, alanine scanning, positional scanning, and scrambled peptide libraries. Price: $18+/peptide. Fast turnaround time: 2 to 3 weeks.
small to large scale production of monoclonal antibodies and recombinant proteins using mammalian cell culture of hybridoma, CHO, NSO, and HEK-293 cells.
Fast stable cell line development services for protein expression: development of suspension CHO stable cell lines expressing recombinant proteins. No virus involved. DNA Transfection and stable cell line screening only.
Fast and high yield stable cell line development services for biologics production: generation of stable cell lines expressing recombinant proteins and antibodies at high yield. Production yield of recombinant antibodies can be >2 g/liter.
Knock-out and knock-in cell lines are generated using the powerful CRISPR/Cas9 genome editing tool. Knock-out and knock-in cell line development with any gene and any host cell, including mammalian primary cell, without introducing foreign DNA.
Custom Chimeric Antigen Receptor (CAR) T-cells are generated by experienced scientists working on antigen and antibody production, antibody engineering, lentivirus, and cell line development services for decades. T cell receptors are engineered to graft an...
DNA Subcloning services: fast return (4 days), any insert and vector size, any template, and any vector, especially for expressing proteins including antibodies in mammalian cells.
We can optimize stable and transient expression for secreted or intracellular, cytosolic or periplasmic, tagged or untagged proteins. We assess expression levels using the methods of SDS-PAGE, Western, ELISA or functional analysis.
