Extensive experience with making DNA constructs for Fc fusion protein production, expressing and purifying a number of Fc fusion proteins from microgram to kilogram scales in mammalian cells, such as CHO and HEK 293 cells.
Our Ad5-RGD and Ad5/35 adenovirus production services include: 1) construction of an Ad5-RGD and Ad5/35 adenovirus vector for expression or silencing of a gene; 2) Ad5-RGD and Ad5/35 adenovirus packaging; 3) amplification and purification of...
Hybridoma antibody sequencing service(hybridoma sequencing, monoclonal antibody cloning, hybridoma cloning): all isotypes (IgG, IgM); antibody variable regions from mouse, rat, armenian hamster, rabbit, human; hybridomas or clonal B cells; sequenced after PCR amplification and DNA...
Our adenovirus production services for toxic genes include: 1) construction of an adenovirus vector for expression of a cytotoxic gene; 2) packaging of adenoviruses carrying cytotoxic genes; 3) amplification and purification of adenoviruses carrying cytotoxic...
Custom lentivirus production services, lentivirus plasmid vector construction, helper-free lentivirus packaging, lentivirus purification by ultracentrifugation, filtration or chromatography, lentivirus titration by p24 ELISA, qPCR or HT1080 cell assay. High yield, high purity, high infectivity. Quality...
Custom AAV production services including AAV plasmid vector construction, helper-free AAV packaging, AAV purification by iodixanol density gradient ultracentrifugation or column chromatography, AAV titration by qPCR. Multiple serotypes. High yield, high purity, high infectivity. Quality...
Custom measurement of endotoxins levels in biological samples such as DNA and protein preparations.
Normal-phase chromatography (NP-HPLC) is an analytical technique that is used to separate molecules on the basis of differences in the strength of their interaction with a polar stationary phase.
Capillary isoelectric focusing is an analytical technique for characterization of protein mixtures and determination of protein isoelectric points.
real-time PCR services (qPCR services, quantitative PCR services or qRT PCR services) for gene expression analysis and gene copy number determination includes RNA or DNA extraction, qPCR primer design and validation, qPCR reaction optimization, and...
