Catalog No.	BP4122
Product Name	Rabbit IgG Fc Protein | BP4122
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	Rabbit Fc protein, Rabbit IgG Fc region, Recombinant rIgG Fc Protein
Summary	The In Vivo Grade Recombinant Rabbit IgG Fc Protein was produced in mammalian cells and purified by protein A. The rIgG Fc protein includes the CH2 (114-213) and CH3 (222-323) domains of the heavy chain constant region as well as the hinge region (105-113) before the CH2 domain.
Form Of Antibody	0.2 μM filtered solution of 1x PBS.
Endotoxin	Less than 1 EU/mg of protein as determined by LAL method.
Purity	>95% by SDS-PAGE under reducing conditions
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

BP4122: Recombinant Rabbit IgG Fc Protein, In Vivo Grade

Background

In vivo grade recombinant natural and engineered rabbit IgG Fc proteins (rIgG Fc), and recombinant rabbit IgG isotype control antibody and mutants are available. Biotinylated rIgG Fc, and rIgG Fc with Avi-, His-, and Flag-tags are also available. Please contact us to ask for a quote for the engineered rabbit IgG Fc proteins, biotinylated rIgG Fc, and rIgG Fc with Avi-, His-, and Flag-tags. The recombinant rabbit IgG Fc protein and mutants can be used as the isotype control of the rIgG antibodies and the rIgG Fc fusion proteins in various in vitro and in vivo experiments.

The in vivo grade recombinant rabbit IgG Fc protein (rIgG Fc, Cys 105 – Lys 323 of the constant region of the IgG heavy chain, Accession # P01870-1) was produced in mammalian cells and purified by protein A. The rIgG Fc protein includes the CH2 (114-213) and CH3 (222-323) domains of the heavy chain constant region as well as the hinge region (105-113) before the CH2 domain.

References of Rabbit IgG Fc Protein:

1、Cytokine-induced translocation of GRP78 to the plasma membrane triggers a pro-apoptotic feedback loop in pancreatic beta cells.
Vig, S., et al. Cell Death Dis. 2019 Apr 5;10(4):309. doi: 10.1038/s41419-019-1518-0. PMID: 30952835.
“INS-1E cells were exposed to anti-GRP78 antibodies against the N-terminal domain (mouse anti-GRP78; #sc-376768; Santa Cruz Biotechnology), C-terminal domain (rabbit anti-GRP78; #ADI-SPA-826-F; Enzo life sciences, Antwerp, Belgium)), control polyclonal rabbit IgG (#BE0095, Bioxcell, NH, USA), and/or recombinant GRP78 (# ADI-SPP-765; Enzo life sciences, Antwerp, Belgium) in the presence of cytokines for 16 h. …sGRP78 can act as a membrane receptor for extracellular, soluble GRP78, as reported in the colorectal adenocarcinoma cell line DLD1. …Interestingly, cytokine-induced ER stress, as evaluated by Chop and Bax mRNA expression, was further enhanced by addition of recombinant GRP78 to the culture medium. …We presently provide the first evidence for a novel pathway of active self-destruction in cytokine-exposed beta cells through the ER chaperone GRP78. …Together with our observation that soluble GRP78 can act as a ligand and activator of sGRP78, these results indicate that beta cells trigger a self-destructive feedback loop when exposed to inflammation, actively participating in their own demise.”

2、Development of an IgG-Fc fusion COVID-19 subunit vaccine, AKS-452.
Alleva, D. G., et al. Vaccine. 2021 Oct 29;39(45):6601-6613. doi: 10.1016/j.vaccine.2021.09.077. PMID: 34642088.
“A murine IgG2a Fc fusion protein with the SP/RBD was expressed in HEK293 cells and was also used for immunizations (muIgG2a-Fc-SP/RBD; SPD-C5259, 600 µg/ml; AcroBiosystems Inc., Newark, DE). …In some experiments, titers were allowed to resolve below 500 µg/mL SP/RBD-specific IgG for a few weeks before receiving a booster injection of AKS-452 without adjuvant. …Serum anti-SP/RBD IgG Ab titers were determined by several types of ELISAs. …Results were reported as mean fluorescent intensity (MFI) units in which a sample was considered positive if it was above the Positive Control IgG MFI. …Furthermore, human and mouse Fc-based Ags induced similar titers demonstrating that foreign sequences of the human IgG1 Fc did not appear to play an enhancing role in this BALB/c mouse immunogenicity model.”

3、Universal fluorescence nanoprobes to enhance the sensitivity of immunochromatographic assay for detection of 17β-estradiol in milk.
Lu, X., et al. Food Chem. 2022 Feb 15;370:131027. doi: 10.1016/j.foodchem.2021.131027. PMID: 34537432.
“Therefore, specific coupling to the Fc region of IgG antibodies is an effective strategy to expose the antigen-binding Fab fragment sufficiently and protect their bioactivity. …Protein G or protein A, targeting the Fc region of IgG antibodies, can serve as a bridge between QBs and antibodies. …Although protein G preferentially binds to the Fc of an IgG antibody, it also binds to Fab. …In addition to label materials, probes preparation methods should be considered in terms of improving sensitivity. …The results show that the proposed ICA based on QBs@SPA and QBs@SPA@anti-IgG probes could detect E2 with IC50 of 8.83 and 0.93 ng/mL, respectively, within 15 min under optimal conditions.”

4、Electrochemical and optical biosensing platforms for the immunorecognition of hazelnut Cor a 14 allergen.
Costa, R., et al. Food Chem. 2021 Nov 1;361:130122. doi: 10.1016/j.foodchem.2021.130122. PMID: 34082386.
“So far, the comparison of IgY and IgG-based systems for allergen detection is very limited (both from plant or animal origin). …The zeta potential is a key physical parameter that describes the surface charge distribution on proteins, which can be used as an indicator of stability since it specifies the degree of repulsion between adjacent charged proteins in a simple dispersion. …This work proposes for the first time the development and comparison of novel biosensing platforms based on the immobilisation of specific polyclonal antibodies onto the gold electrode surface to detect trace amounts of hazelnut Cor a 14 allergen (fg concentration level) using electrochemical and optical transducers. …Both approaches were developed with two types of custom-made antibodies, namely anti-Cor a 14 IgG (rabbit) and anti-Cor a 14 IgY (hen’s egg) targeting the Cor a 14 allergen. …Preliminary results of the application of anti-Cor a 14 IgY electrochemical immunosensor to incurred foods established a LOD of 1 mg kg−1 of hazelnut in wheat (0.16 mg kg−1 hazelnut protein).”

5、An Enhanced Bimetallic Optical Fiber SPR Biosensor Using Graphene Oxide for the Label-Free and Sensitive Detection of Human IgG.
Xu, Q., et al. Sensors (Basel). 2025 Mar 6;25(5):1630. doi: 10.3390/s25051630. PMID: 40096495.
“Immunoglobulin G (IgG) is the most important class of antibodies in the body’s immune system. …The sensor in our study can create an enhanced surface plasmon resonance effect through gold and silver bimetallic nanoparticles and graphene oxide modification, which can amplify the sensing signal and greatly improve the sensitivity of detection. …Rabbit anti-human IgG antibody, human IgG, and goat IgG were purchased from Shanghai Zhuoshi Biotechnology Co (Shanghai, China). …In order to achieve modification on the surface of the fiber, the unbound rabbit anti-human IgG was rinsed with PBS solution, and finally, the binding site on the surface of the fiber was closed with ethanolamine solution to block the unbound carboxyl group. …In order to evaluate the specificity and selectivity of the assay, we used the same method as that of human IgG detection to detect multicomponent solutions.”

For more references about Rabbit IgG Fc Protein please contact our scientific support team with message@sydlabs.com.

Other Recombinant IgG Fc Proteins:
Recombinant Human IgG1 Fc Protein (hIgG1), In vivo Grade
Recombinant Human IgG2 Fc Protein (hIgG2), In vivo Grade
Recombinant Human IgG4 Fc Protein (hIgG4), In vivo Grade
Recombinant Mouse IgG1 Fc Protein (mIgG1), In vivo Grade
Recombinant Mouse IgG2a Fc Protein (mIgG2a), In vivo Grade
Recombinant Mouse IgG2b Fc Protein (mIgG2b), In vivo Grade
Recombinant Mouse IgG2c Fc Protein (mIgG2c), In vivo Grade
Recombinant Mouse IgG3 Fc Protein (mIgG3), In vivo Grade
Recombinant Rat IgG2a Fc Protein (rtIgG2a), In vivo Grade
Recombinant Rat IgG2b Fc Protein (rtIgG2b), In vivo Grade
Recombinant Llama IgG2b Fc Protein (lIgG2b), In vivo Grade
Recombinant Rabbit IgG Fc Protein (rIgG), In vivo Grade

Recombinant IgG Reference Antibodies:
Recombinant Human IgG1 Isotype Control Antibody and Mutants, In vivo Grade
Recombinant Human IgG2 Isotype Control Antibody, In vivo Grade
Recombinant Human IgG3 Isotype Control Antibody, In vivo Grade
Recombinant Human IgG4-S228P Isotype Control Antibody and Mutants, In vivo Grade
Recombinant Mouse IgG1 Isotype Control Antibody and Mutants, In vivo Grade
Recombinant Mouse IgG2a Isotype Control Antibody and Mutants, In vivo Grade
Recombinant Mouse IgG2b Isotype Control Antibody and Mutants, In vivo Grade
Recombinant Mouse IgG2c Isotype Control Antibody and Mutants, In vivo Grade
Recombinant Mouse IgG3 Isotype Control Antibody, In vivo Grade
Recombinant Rat IgG1 Isotype Control Antibody, In vivo Grade
Recombinant Rat IgG2a Isotype Control Antibody, In vivo Grade
Recombinant Rat IgG2b Isotype Control Antibody, In vivo Grade
Recombinant Rat IgG2c Isotype Control Antibody, In vivo Grade

Fc ELISA Kits and Reagents:
Human Fc ELISA Kit
Mouse Fc ELISA Kit
Human Fc ELISA Reagent Kit
Mouse Fc ELISA Reagent Kit

Rabbit IgG Fc Protein from: In Vivo Grade Recombinant Rabbit IgG Fc Protein: BP4122 Syd Labs

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