Mouse IgG1-D265A Isotype Control Antibody PA007135.05 Ushelf

Mouse IgG1-D265A Isotype Control Antibody (Clone: 12B9) | PA007135.05

$450.00 – $1,200.00

Recombinant mouse IgG1 kappa isotype control and mIgG1 D265A isotype control mutant for in vitro and in vivo studies. Low or no specific binding to mouse samples tested. More choices of recombinant mouse IgG1 isotype controls, including targets, mutants, tags, and conjagates.

Details & Specifications
References

Catalog No.	PA007135.05
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	Recombinant Mouse IgG1 Isotype Control, Mouse IgG1 Negative Control Antibody, mIgG1 Isotype Control
Summary	The In Vivo Grade Recombinant Mouse IgG1-D265A Isotype Control Antibody, Endotoxin 0.05 EU/mg was produced in mammalian cells.
Isotype	mouse IgG1, kappa
Applications	an isotype-matched negative control for mouse IgG1 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Immunogen	N/A.
Form Of Antibody	0.2 μM filtered solution of 1x PBS.
Endotoxin	< 0.05 EU per 1 mg of protein as determined by LAL method.
Purity	>95% by SDS-PAGE under reducing conditions.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007135.05: Recombinant Mouse IgG1-D265A Isotype Control Antibody (Clone: 12B9), In Vivo Grade

Mouse IgG1 isotype control and mutants (natural and engineered mIgG1 isotype controls) are useful for in vitro and in vivo studies from ELISA, Western blot (WB), to cell-based assay to animal model study. The isotypes of mouse IgG antibody reagents used in scientific research and clinical diagnostics are mainly mouse IgG1 kappa. Mouse IgG1 antibodies and engineered mutants are also used in therapeutic drug discovery. Mouse IgG1 antibodies are more like human IgG4 antibodies than human IgG1 antibodies, which behave more like mouse IgG2a or IgG2c antibodies. Many antibody drugs especially those targetting immune checkpoint proteins use the human IgG4 constant regions.

Please contact us to ask for a quote for the Fc silenced mouse IgG1 isotype control mutants, mIgG1 isotype control and mutants with Avi-, His-, and Flag-tags, and biotinylated mIgG1 isotype control and mutants. A variety of conjugates (such as dyes and fluorophores) with the mouse IgG1 isotype control and Fc-slient mutants are available.

Background

Mouse IgG antibodies are classified to four subclasses mainly based on the isotype of the heavy chain (IgG1, IgG2a or IgG2c, IgG2b, and IgG3 for the heavy chain; kappa and lamda for the light chain), of which IgG1 and kappa are the most abundantnamed in serum. IgG antibodies are large molecules of about 150 kDa composed of four peptide chains, two identical heavy chains of about 50 kDa and two identical light chains of about 25 kDa. The four peptides are arranged in a Y-shape tetramer by disulfide bonds formed between the two heavy chains and between a heavy chain and a light chain.

The in vivo grade recombinant mouse IgG1-D265A isotype control antibody (mIgG1 D265A isotype control) was produced in mammalian cells.

References of Mouse IgG1 Isotype Control Antibody

1. In vivo targeting of open prothrombin with the monoclonal antibody POmAb results in anticoagulation without excessive bleeding.
Brake, M. A., et al.J Thromb Haemost. 2026 Feb 13:S1538-7836(26)00111-X. PMID: 41692091
“The in vivo grade recombinant mouse IgG1 antibody from Syd Labs (PA007126) was used as a control in all the in vitro, ex vivo, and in vivo studies.”

2. Intravenous anti-Aβ immunotherapy acutely increases cerebral amyloid angiopathy and vascular damages in APOE4 mice.
Rebeck, G. W., et al.bioRxiv [Preprint]. 2026 Feb 11:2026.02.09.704876. PMID: 41727014; PMCID: PMC12918975
“3D6 Immunotherapy treatment paradigm: Mice have been divided into 8 groups (N=7-8 each): APOE3 mice treated with IgG1 isotype control, APOE3 mice treated with 5mg/kg IV 3D6 …Four-month-old mice received a single 5mg/kg IV injection of either 3D6 or IgG1 isotype control antibody (Syd Labs cat #PA007126) into the lateral tail vein …The other hemisphere was snap frozen on dry ice and stored at −80°C for biochemical analyses. The fixed hemisphere was sliced into 30μm sections on microtome for immunofluorescence (IF) experiments.”

3. POmAb, an antibody targeting open prothrombin, results in anticoagulation without excessive bleeding in mice.
Pozzi, N., et al.bioRxiv [Preprint]. 2025 Jul 4:2025.06.29.662207. PMID: 40631305; PMCID: PMC12236754
“POmAb was expressed in CHO cells and purified as described previously. Purity was >95% (4–12% SDS-PAGE under non-reducing conditions) and 99% (SEC-HPLC). Endotoxin level was < 0.1 EU/mg. POmAb concentration was calculated using the following molecular weight and extinction coefficient (E280nm0.1%): 150,000 Da, 1.58. In vivo grade recombinant mouse IgG1 isotype control antibody was from Syd Labs (PA007126). Antibodies were aliquoted and stored at -80°C until used. Fluorescently labeled POmAb was prepared by conjugating 1.3 mg of antibody in 0.5 mL of 50 mM sodium borate buffer (pH 8.5) with DyLight 650-NHS ester. Protein/dye ratio was 3.27 moles of dye per mole of protein. Anti-fibrin antibody was purified from a growing hybridoma clone 59D8. Anti-CD42b beta antibody for staining platelets was from Emfret Analytics. All other reagents were purchased from Invitrogen (Carlsbad, CA) and Sigma (St. Louis, MO) unless otherwise stated.”

For more technical references or data sheets regarding the Mouse IgG1 Isotype Control Antibody formats, please contact our scientific support team at message@sydlabs.com or explore options via Syd Labs.