Biotinylated Anti-human CD3e Antibody (UCHT1, Human IgG1 Fc Silent Kappa)
$45.00 – $90.00
Recombinant human anti-human CD3 monoclonal antibody (Clone UCHT1, Cat. No. PA007223.h1Fs-b), engineered with a Human IgG1 Fc-Silent domain and conjugated with Biotin to minimize non-specific Fc receptor binding and background noise during flow cytometric analysis. Variable regions are derived from clone UCHT1 and produced in mammalian cell culture.
- Conjugate & Isotype: Biotin, Human IgG1 Fc-Silent
- Applications: Flow Cytometry (FC), Cell Surface Staining, T Cell Immunophenotyping
- Alternative Formats: Unconjugated formats, fluorescent dye conjugates, and alternative clones (OKT3, SP34-2, 12F6) are also available.
- Details & Specifications
- References
| Catalog No. | PA007223.h1Fs-b |
|---|---|
| Product Name | Biotinylated Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1), Human IgG1 Fc Silent Kappa |
| Supplier Name | Syd Labs, Inc. |
| Brand Name | Syd Labs |
| Clone | UCHT1. |
| Isotype | Human IgG1 Fc Silent Kappa. |
| Source/Host | The site-directed biotinylated anti-human CD3 monoclonal antibody (clone: UCHT1) was produced in mammalian cells. |
| Specificity/Sensitivity | The site-directed biotinylated recombinant UCHT1 antibody specifically binds to the human T cell receptor CD3. |
| Applications | Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads. |
| Form Of Antibody | 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives. |
| Conjugate | biotin-conjugated. |
| Purity | >95% by SDS-PAGE under reducing conditions and HPLC. |
| Shipping | The antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied. |
| Note | Recombinant mouse IgG1 isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator. |
| Order Offline | Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card. |
Description
PA007223.h1Fs-b: Biotinylated Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1), Human IgG1 Fc Silent Kappa
Recombinant human IgG1 monoclonal antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
References of Anti-Human CD3 Monoclonal Antibody (Clone: UCHT1) for Flow Cytometry
Rao PN, et al. Clin Transl Immunol. 2022. doi: 10.1002/cti2.1415
“…The assay uses a lyophilized reagent containing PE/Cy7-conjugated anti-human CD3 antibody (clone UCHT1) for T cells and APC/Fire 750-conjugated anti-human CD19 antibody (clone HIB19) for B cells. Cells were stained with a six-color lyophilized antibody reagent and analyzed on a BD FACSLyric system…”
Lind Enoksson S, et al. J Immunol Methods. 2021. doi: 10.1016/j.jim.2021.113159
“…CD3+ T-lymphocytes were analyzed using an Aquios CL flow cytometer with a monoclonal antibody mixture including anti-CD3-PC5 (clone UCHT1). Samples were stained with CD3-FITC/CD4-PE Simultest, and blasts were identified as high FSC cells…”
Frequently Asked Questions (FAQ) – Recombinant CD3 (UCHT1) For Flow Cytometry
Q: How do Syd Labs’ Human IgG1 Fc Silent formats reduce background noise in complex multicolor flow cytometry assays?
A classic bottleneck in immune cell gating is the non-specific binding of antibody Fc regions to cellular Fcγ receptors (FcγRs) on monocytes, macrophages, and NK cells, which triggers false-positive fluorescence signals. Our innovative fc silent anti-human cd3 antibody (such as catalog series PA007222.h1Fs) contains engineered mutations within the human IgG1 constant domain that eliminate binding affinity to FcγRs. This design allows investigators to skip expensive, time-consuming Fc-block pre-treatment steps while executing ultra-clean recombinant anti-human cd3 ucht1 flow cytometry gating cascades.
Q: When should I choose the Biotinylated Human IgG1 Fc Silent format for my lymphocyte panel?
Our biotinylated recombinant anti-human cd3 ucht1 configuration (PA007233.h1Fs-b) is specifically optimized for high-sensitivity multi-color flow cytometry and indirect signal amplification. By leveraging a secondary Streptavidin-fluorophore conjugate step, it provides an outstanding signal-to-noise ratio for detecting low-abundance T-cell sub-compartments. Furthermore, this format provides excellent multi-color panel design flexibility, allowing you to easily shift your CD3 fluorophore channels independent of standard direct-conjugate restrictions without introducing Fc-binding artifacts.
Q: What are the exact host species, subunit targets, and isotype matching recommendations for these UCHT1 clones?
Syd Labs offers structured catalog diversity to match distinct assay controls perfectly. Our standard recombinant platform delivers the classic anti-human cd3 clone ucht1 mouse igg1 (kappa) format (PA007222.m1), which targets the anti-human cd3e monoclonal antibody flow cytometry epsilon chain subunit. If your experimental pipeline requires an all-human constant framework, we provide the engineered Human IgG1 Fc Silent Kappa platform. This defined recombinant nature guarantees 100% sequence uniformity across lots, ensuring that your isotype control baselines remain flawlessly consistent from one screening sprint to the next.
Syd Labs provides the following recombinant anti-human CD3 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
Biotin Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
FITC Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
APC Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
PE Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
Pacific Blue Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
PerCP Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
PE/Cy5 Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
PE/Cy7 Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
iFluor 488 Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
iFluor 549 Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
iFluor 594 Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
iFluor 647 Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
iFluor 700 Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
APC/Cy7 Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
PerCP/Cy5.5 Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
Syd Labs provides the following in vivo grade recombinant anti-human CD3 bispecific antibodies:
Recombinant Anti-human CD3 / CD3 Bispecific Antibody (Clone: OKT3 / UCHT1)
Recombinant Anti-human CD3 / CD3 Bispecific Antibody (Clone: OKT3 / SP34-2)
Recombinant Anti-human CD3 / CD3 Bispecific Antibody (Clone: SP34-2 / OKT3)
Syd Labs provides the following in vivo grade recombinant anti-mouse CD3 monoclonal antibodies:
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 145-2C11)
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 500A2)
Recombinant Anti-mouse CD3 monoclonal antibody (Clone: 17A2)
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: KT3)
Syd Labs provides the following recombinant anti-human CD3 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD3 monoclonal antibody (Clone: OKT3) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: UCHT1) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: 12F6) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: SP34-2) for flow cytometry
Syd Labs provides the following recombinant anti-mouse CD3 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD3 monoclonal antibody (Clone: 17A2) for flow cytometry
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 145-2C11) for flow cytometry
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 500A2) for flow cytometry
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: KT3) for flow cytometry
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