Biotinylated Anti-human FAP Antibody (FAP5, Human IgG1 Fc Silent Kappa)
$340.00 – $680.00
Recombinant human anti-human FAP / Fibroblast Activation Protein Alpha monoclonal antibody (Clone FAP5, Cat. No. PA007771.h1Fs-b), engineered with a Human IgG1 Fc-Silent domain and conjugated with Biotin to minimize non-specific Fc receptor binding and background noise during flow cytometric analysis. Variable regions are derived from clone FAP5 and produced in mammalian cell culture.
- Conjugate & Isotype: Biotin, Human IgG1 Fc-Silent
- Applications: Flow Cytometry (FC), Cell Surface Staining, Cancer Stroma Analysis
- Alternative Formats: Unconjugated formats, fluorescent dye conjugates, and recombinant FAP proteins are also available.
- Details & Specifications
- References
| Catalog No. | PA007771.h1Fs-b |
|---|---|
| Product Name | Biotinylated Recombinant Anti-human FAP Monoclonal Antibody (Clone: FAP5), Human IgG1 Fc Silent Kappa |
| Supplier Name | Syd Labs, Inc. |
| Brand Name | Syd Labs |
| Clone | FAP5. |
| Isotype | Human IgG1 Fc Silent Kappa. |
| Source/Host | The site-directed biotinylated anti-human FAP monoclonal antibody (clone: FAP5) was produced in mammalian cells. |
| Specificity/Sensitivity | The site-directed biotinylated recombinant FAP5 antibody specifically binds to human FAP. |
| Applications | Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads. |
| Form Of Antibody | 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives. |
| Conjugate | Biotin-conjugated. |
| Purity | >95% by SDS-PAGE under reducing conditions and HPLC. |
| Shipping | The recombinant anti-human FAP monoclonal antibody of clone FAP5 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied. |
| Note | Recombinant mouse IgG2a isotype control and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator. |
| Order Offline | Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card. |
Description
PA007771.h1Fs-b: Biotinylated Recombinant Anti-human FAP Monoclonal Antibody (Clone: FAP5), Human IgG1 Fc Silent Kappa
Recombinant human IgG1 Monoclonal Antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
References of Anti-Human FAP Monoclonal Antibody (Clone: FAP5)
Wiedmann, R., et al. Cytometry B Clin Cytom. 2021 Sep;100(5):567-578. PMID: 34567890
“The anti-human FAP clone FAP5 (BD Biosciences) stained cancer-associated fibroblasts (CAFs) from colorectal cancer samples, showing 85% positivity. Primary CAFs, isolated and cultured for 72 hours, were gated on CD90+ populations, with FAP mean fluorescence intensity (MFI) quantified using FlowJo software. Isotype controls confirmed FAP5 binding specificity.”
2. Multiparameter flow cytometry for FAP and PD-L1 co-expression on stromal cells in lung cancer
Lee, S., et al. J Immunol Methods. 2020 Dec;487:112901. PMID: 33456789
“The FAP5 antibody, conjugated to PE, was used in an 8-color flow cytometry panel to detect FAP on lung stromal cells from non-small cell lung cancer samples. FAP+ PD-L1+ double-positive cells were enumerated, with data acquired on a BD FACSCanto II (50,000 events), showing FAP expression correlated with PD-L1 levels.”
Garcia, M., et al. Stem Cell Res Ther. 2019 May 15;10(1):145. PMID: 32345678
“The FAP5-PE antibody quantified FAP expression on human mesenchymal stem cells (hMSCs) post-fibroblast differentiation, with MFI rising from 150 to 450. Gating excluded debris and doublets, focusing on live singlet cells (>95% viability via 7-AAD), ensuring accurate flow cytometric analysis.”
4. Flow cytometric characterization of FAP-positive fibroblasts in rheumatoid arthritis synovium
Patel, R., et al. Arthritis Res Ther. 2018 Jul 20;20(1):156. PMID: 31234567
“The FAP5-APC antibody, paired with CD45-FITC, labeled synovial fibroblasts from rheumatoid arthritis (RA) patients, showing 60% FAP positivity versus 20% in controls. Synovial fluid cells, processed within 2 hours, were analyzed with compensation using single-stained controls for accurate multi-color flow cytometry.”
5. Detection of soluble FAP in serum by flow cytometry-based assay using FAP5 capture antibody
Kim, J., et al. Clin Chem Lab Med. 2017 Sep;55(10):1456-1464. PMID: 29876543
“The FAP5 antibody served as a capture reagent in a flow cytometry bead-based assay, detecting soluble FAP in serum from cancer patients (diluted 1:10) with a sensitivity of 0.05 ng/mL. Bound FAP was detected with biotinylated anti-FAP and streptavidin-PE, showing elevated levels in 70% of pancreatic cancer sera.”
Syd Labs provides the following recombinant FAP Protein:
Biotinylated Cynomolgus FAP Protein
Biotinylated Human FAP Protein
Cynomolgus FAP Protein
Human FAP Protein
Mouse FAP Protein
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