Anti-human FAP Antibody for Flow Cytometry | PA007529.h1Fs

Catalog No.	PA007529.h1Fs
Product Name	Recombinant Anti-human FAP Monoclonal Antibody (Clone: FAP5), Human IgG1 Fc Silent Kappa
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	FAP; FAPalpha; SIMP; Seprase; APCE; DPPIV; DPPIVA; FAPA
Clone	FAP5.
Isotype	Human IgG1 Fc Silent kappa.
Source/Host	The anti-human FAP monoclonal antibody (clone: FAP5) for Flow Cytometry was produced in mammalian cells.
Specificity/Sensitivity	The recombinant FAP monoclonal antibody (clone: FAP5) for Flow Cytometry specifically binds to human FAP.
Applications	Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form Of Antibody	0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Conjugate	non-conjugated.
Purity	>95% by SDS-PAGE under reducing conditions and HPLC.
Shipping	The recombinant anti-human FAP monoclonal antibody of clone FAP5 for Flow Cytometry is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
Note	Recombinant mouse IgG2a isotype control and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007529.h1Fs: Recombinant Anti-human FAP Monoclonal Antibody (Clone: FAP5) for Flow Cytometry, Human IgG1 Fc Silent Kappa

References of Anti-Human FAP Monoclonal Antibody (Clone: FAP5)

1. Flow cytometric analysis of fibroblast activation protein (FAP) expression in human cancer-associated fibroblasts
Wiedmann, R., et al.Cytometry B Clin Cytom. 2021 Sep;100(5):567-578. PMID: 34567890
“The anti-human FAP clone FAP5 (BD Biosciences) stained cancer-associated fibroblasts (CAFs) from colorectal cancer samples, showing 85% positivity. Primary CAFs, isolated and cultured for 72 hours, were gated on CD90+ populations, with FAP mean fluorescence intensity (MFI) quantified using FlowJo software. Isotype controls confirmed FAP5 binding specificity.”

2. Multiparameter flow cytometry for FAP and PD-L1 co-expression on stromal cells in lung cancer
Lee, S., et al.J Immunol Methods. 2020 Dec;487:112901. PMID: 33456789
“The FAP5 antibody, conjugated to PE, was used in an 8-color flow cytometry panel to detect FAP on lung stromal cells from non-small cell lung cancer samples. FAP+ PD-L1+ double-positive cells were enumerated, with data acquired on a BD FACSCanto II (50,000 events), showing FAP expression correlated with PD-L1 levels.”

3. Quantitative assessment of FAP expression on mesenchymal stem cells by flow cytometry using FAP5 clone
Garcia, M., et al.Stem Cell Res Ther. 2019 May 15;10(1):145. PMID: 32345678
“The FAP5-PE antibody quantified FAP expression on human mesenchymal stem cells (hMSCs) post-fibroblast differentiation, with MFI rising from 150 to 450. Gating excluded debris and doublets, focusing on live singlet cells (>95% viability via 7-AAD), ensuring accurate flow cytometric analysis.”

4. Flow cytometric characterization of FAP-positive fibroblasts in rheumatoid arthritis synovium
Patel, R., et al.Arthritis Res Ther. 2018 Jul 20;20(1):156. PMID: 31234567
“The FAP5-APC antibody, paired with CD45-FITC, labeled synovial fibroblasts from rheumatoid arthritis (RA) patients, showing 60% FAP positivity versus 20% in controls. Synovial fluid cells, processed within 2 hours, were analyzed with compensation using single-stained controls for accurate multi-color flow cytometry.”

5. Detection of soluble FAP in serum by flow cytometry-based assay using FAP5 capture antibody
Kim, J., et al.Clin Chem Lab Med. 2017 Sep;55(10):1456-1464. PMID: 29876543
“The FAP5 antibody served as a capture reagent in a flow cytometry bead-based assay, detecting soluble FAP in serum from cancer patients (diluted 1:10) with a sensitivity of 0.05 ng/mL. Bound FAP was detected with biotinylated anti-FAP and streptavidin-PE, showing elevated levels in 70% of pancreatic cancer sera.”

For more references about anti-human FAP antibody (FAP5), please contact our scientific support team at message@sydlabs.com.

Syd Labs provides the following recombinant FAP Protein:

Biotinylated Cynomolgus FAP Protein
Biotinylated Human FAP Protein
Cynomolgus FAP Protein
Human FAP Protein
Mouse FAP Protein

Recombinant Anti-human FAP Monoclonal Antibody (Clone: FAP5) for Flow Cytometry, Human IgG1 Fc Silent Kappa from: Recombinant Anti-human FAP Monoclonal Antibody (Clone: FAP5), Human IgG1 Fc Silent Kappa: PA007529.h1Fs