Biotinylated Anti-human CD71 Antibody (OKT9, Human IgG1 Fc Silent Kappa)
$110.00 – $220.00
Recombinant human anti-human CD71 / Transferrin Receptor 1 (TfR1) monoclonal antibody (Clone OKT9, Cat. No. PA007770.h1Fs-b), engineered with a Human IgG1 Fc-Silent domain and conjugated with Biotin to minimize non-specific Fc receptor binding and background noise during flow cytometric analysis. Variable regions are derived from the classic OKT9 clone and produced in mammalian cell culture.
- Conjugate & Isotype: Biotin, Human IgG1 Fc-Silent
- Applications: Flow Cytometry (FC), Cell Surface Staining, Immunophenotyping
- Alternative Formats: Unconjugated formats, fluorescent dye conjugates, and alternative clones (5E9C11, B3/25) are also available.
- Details & Specifications
- References
| Catalog No. | PA007770.h1Fs-b |
|---|---|
| Product Name | Biotinylated Recombinant Anti-human CD71 Monoclonal Antibody (Clone: OKT9), Human IgG1 Fc Silent Kappa |
| Supplier Name | Syd Labs, Inc. |
| Brand Name | Syd Labs |
| Clone | OKT9. |
| Isotype | Human IgG1 Fc Silent Kappa. |
| Source/Host | The site-directed biotinylated anti-human CD71 monoclonal antibody (clone: OKT9) was produced in mammalian cells. |
| Specificity/Sensitivity | The site-directed biotinylated recombinant OKT9 antibody specifically binds to human CD71. |
| Applications | Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads. |
| Form Of Antibody | 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives. |
| Conjugate | Biotin-conjugated. |
| Purity | >95% by SDS-PAGE under reducing conditions and HPLC. |
| Shipping | The recombinant anti-human CD71 monoclonal antibody of clone OKT9 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied. |
| Note | Recombinant mouse IgG1 isotype control and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator. |
| Order Offline | Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card. |
Description
PA007770.h1Fs-b: Biotinylated Recombinant Anti-human CD71 Monoclonal Antibody (Clone: OKT9), Human IgG1 Fc Silent Kappa
Recombinant human IgG1 Monoclonal Antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
References of Anti-Human CD71 Monoclonal Antibody (OKT9)
Gaurav I, et al. Pharmaceutics. 2021 Sep 9;13(9):1433. PMID: 34575511
“The OKT9 monoclonal antibody targeted TfR1 in flow cytometry studies of cancer cells, showing increased fluorescence intensity in hypoxic conditions and correlating with high TfR1 expression in proliferating tumor cells. Internalization assays confirmed OKT9-mediated uptake, and therapeutic conjugates demonstrated selective cytotoxicity in vitro.”
2. CD71 expression in large cell anaplastic lymphomas: an immunohistochemical and flow cytometric study
Falini B, et al. Am J Clin Pathol. 1991 Aug;96(2):265-72. PMID: 1856360
“The OKT9 antibody (equivalent to B3/25) assessed CD71 expression in flow cytometry, revealing positivity in 80% of large cell anaplastic lymphomas. Staining patterns varied between anaplastic and diffuse large cell subtypes, with immunohistochemistry showing membrane accentuation and high CD71 linked to aggressive disease prognosis.”
3. Flow cytometric analysis of the transferrin receptor in human leukemia cells
Taetle R, et al. Leukemia. 1986 Jun;1(6):403-9. PMID: 3011823
“The OKT9 monoclonal antibody was used for flow cytometric detection of transferrin receptors on leukemia blasts, with receptor density quantified via mean channel fluorescence. Staining intensity correlated with cell proliferation rates, and blocking with excess transferrin reduced OKT9 binding, showing heterogeneity across leukemia subtypes.”
Newman RA, et al. Int J Cancer. 1983 Mar 15;31(3):311-5. PMID: 6131211
“The OKT9 antibody’s flow cytometry binding correlated with histological class in non-Hodgkin lymphomas, showing higher reactivity in high-grade lymphomas. Flow analysis confirmed receptor expression on tumor lymphocytes, with tissue sections validating findings and survival data indicating prognostic value of OKT9 levels.”
Loken MR, et al. Blood. 1982 Nov;60(5):1099-108. PMID: 6186112
“The OKT9 antibody measured transferrin receptor expression on hematopoietic cells via flow cytometry, showing increased expression in the erythroid lineage and bimodal distribution in leukemic blasts. Quantitative analysis revealed significant correlation with proliferation markers, distinguishing normal from leukemic populations.”
Syd Labs provides the following in vivo grade recombinant anti-human CD71 monoclonal antibodies:
Recombinant Anti-human CD71 monoclonal antibodies (Clone: OKT9)
Recombinant Anti-human CD71 (TfR) monoclonal antibodies (Clone: 5E9C11)
Recombinant Anti-human CD71 (TfR1) monoclonal antibodies (Clone: B3/25)
Syd Labs provides the following recombinant anti-human CD71 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD71 monoclonal antibodies (Clone: OKT9) for flow cytometry
Syd Labs provides the following in vivo grade recombinant anti-mouse CD71 monoclonal antibodies:
Recombinant Anti-mouse CD71 (TfR1) monoclonal antibodies (Clone: R17217.1.3)
Syd Labs provides the following recombinant CD71 Protein:
Biotinylated Human Transferrin R Protein
Cynomolgus Transferrin R Protein
Human Transferrin R Protein
Mouse Transferrin R Protein
You may also like…
- -10%Select optionsQuick ViewProteins & Peptides, Recombinant Protein
Human Transferrin R/CD71 Protein (TFR-HM201)
$202.50 – $675.00 - -10%Select optionsQuick ViewProteins & Peptides, Recombinant Protein
Human Transferrin R/CD71 Protein (TFR-HM401)
$243.00 – $810.00 - Select optionsQuick View
- Select optionsQuick View
- -10%Select optionsQuick ViewProteins & Peptides, Recombinant Protein
Mouse Transferrin R/CD71 Protein (TFR-MM101)
$283.50 – $945.00 - Select optionsQuick View
- Select optionsQuick View
- Select optionsQuick View
- -10%Select optionsQuick View