Catalog No.	PA007142.05
Product Name	Rat IgG1 Isotype Control Antibody (Clone: 12B9) | PA007142.05
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	Recombinant Rat IgG1 Isotype Control, Rat IgG1 Negative Control Antibody, rtIgG1 Isotype Control
Summary	The In Vivo Grade Recombinant Rat IgG1 Isotype Control Antibody, Endotoxin 0.05 EU/mg was produced in CHO cells.
Isotype	rat IgG1, kappa
Applications	an isotype-matched negative control for rat IgG1 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Immunogen	N/A.
Form Of Antibody	0.2 μM filtered solution of 1x PBS.
Endotoxin	< 0.05 EU per 1 mg of protein as determined by LAL method.
Purity	>95% by SDS-PAGE under reducing conditions.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007142.05: Recombinant Rat IgG1 Isotype Control Antibody (Clone: 12B9), In Vivo Grade

Rat IgG1 isotype control and mutants (natural and engineered rtIgG1 isotype controls) are useful for functional study using animal model among a number of in vitro and in vivo research applications from ELISA, Western Blot (WB) to cell-based assays. Rat IgG1 and IgG2a antibodies are more like mouse IgG1 than rat IgG2b antibodies. Most of rat antibodies are used to study mouse target proteins.

Please contact us to ask for a quote for the engineered such as Fc silenced rat IgG1 isotype control mutants, rtIgG1 isotype control and mutants with Avi-, His-, and Flag-tags, and biotinylated rIgG1 isotype control and mutants. A variety of conjugates (such as dyes and fluorophores) with the rat IgG1 isotype control and Fc silenced mutants are available.

Background

Rat IgG antibodies are classified to four subclasses mainly based on the isotype of the heavy chain (IgG1, IgG2a, IgG2b, and IgG2c for the heavy chain; kappa and lamda for the light chain), of which IgG2b and IgG2a, and kappa are the most abundantnamed in serum. IgG antibodies are large molecules of about 150 kDa composed of four peptide chains, two identical heavy chains of about 50 kDa and two identical light chains of about 25 kDa. The four peptides are arranged in a Y-shape tetramer by disulfide bonds formed between the two heavy chains and between a heavy chain and a light chain.

Rat IgG1 antibodies can be purified by the protein G beads, and relatively lower yield by the protein A beads.

The in vivo grade recombinant rat IgG1 isotype control antibody (rtIgG1 isotype control) was produced in CHO cells.

Other in vivo grade Recombinant IgG Isotype Control Antibodies and Mutants:
Recombinant Human IgG1 Isotype Control Antibody and Mutants, In vivo Grade
Recombinant Human IgG2 Isotype Control Antibody, In vivo Grade
Recombinant Human IgG3 Isotype Control Antibody, In vivo Grade
Recombinant Human IgG4-S228P Isotype Control Antibody and Mutants, In vivo Grade
Recombinant Mouse IgG1 Isotype Control Antibody and Mutants, In vivo Grade
Recombinant Mouse IgG2a Isotype Control Antibody and Mutants, In vivo Grade
Recombinant Mouse IgG2b Isotype Control Antibody and Mutants, In vivo Grade
Recombinant Mouse IgG2c Isotype Control Antibody and Mutants, In vivo Grade
Recombinant Mouse IgG3 Isotype Control Antibody, In vivo Grade
Recombinant Rat IgG1 Isotype Control Antibody, In vivo Grade
Recombinant Rat IgG2b Isotype Control Antibody, In vivo Grade
Recombinant Rat IgG2c Isotype Control Antibody, In vivo Grade
Recombinant Hamster IgG1 Isotype Control Antibody， In vivo Grade
Recombinant Hamster IgG2 Isotype Control Antibody， In vivo Grade
Recombinant Rat IgG2c Isotype Control Antibody, In vivo Grade
Recombinant Hamster IgG1 Isotype Control Antibody, In vivo Grade
Recombinant Hamster IgG2 Isotype Control Antibody, In vivo Grade

In vivo Grade Recombinant IgG Fc Proteins:
Recombinant Human IgG1 Fc Protein (hIgG1), In vivo Grade
Recombinant Human IgG2 Fc Protein (hIgG2), In vivo Grade
Recombinant Human IgG4 Fc Protein (hIgG4), In vivo Grade
Recombinant Mouse IgG1 Fc Protein (mIgG1), In vivo Grade
Recombinant Mouse IgG2a Fc Protein (mIgG2a), In vivo Grade
Recombinant Mouse IgG2b Fc Protein (mIgG2b), In vivo Grade
Recombinant Rat IgG2a Fc Protein (rtIgG2a), In vivo Grade
Recombinant Rat IgG2b Fc Protein (rtIgG2b), In vivo Grade
Recombinant Llama IgG2b Fc Protein (lIgG2b), In vivo Grade
Recombinant Rabbit IgG Fc Protein (rIgG), In vivo Grade

Rat IgG1 Isotype Control (12B9) from: In Vivo Grade Recombinant Rat IgG1 Isotype Control Antibody, Endotoxin 0.05 EU/mg: PA007142.05 Syd Labs

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