Mycoplasma Mycoplasma DNA Detection Kit (2G)
HZSKBIO Mycoplasma DNA Detection Kit (2G) (1509841) is used, together with MycoSHENTEK Mycoplasma DNA Extraction Kit (2G), to qualitatively determine whether there is mycoplasma, spiroplasma, or acholeplasma contamination in master cell bank, working cell bank, virus seed stock, or cell culture-derived products, etc.
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| Catalog No. | 1509841 |
|---|---|
| Product Name | Mycoplasma Mycoplasma DNA Detection Kit (2G) |
| Supplier Name | HZSKBIO |
| Brand Name | MycoSHENTEK® |
| Shipping | Shipped at Room Temp. |
| Order Offline | Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Orleave a message with a formal purchase order (PO) Or credit card. |
Description
1509841: MycoSHENTEK® Mycoplasma DNA Detection Kit (2G)
Product Overview
MycoSHENTEKTM Mycoplasma DNA Detection Kit (2G) is used, together with MycoSHENTEKTM Mycoplasma DNA Extraction Kit (2G), to qualitatively determine whether there is mycoplasma, spiroplasma, or acholeplasma contamination in master cell bank, working cell bank, virus seed stock, or cell culture-derived products, etc. The integrated system has been fully validated per European Pharmacopoeia Chapter 2.6.7 and Japanese Pharmacopoeia XVII, at a detection limit of 10 CFU/mL. The detection kit uses real-time PCR technology to detect more than 200 species of Mycoplasma, Spiroplasma and Acholeplasma with high specificity. It has been validated in a variety of matrices, as well as on non-mycoplasma species, production cells, and engineered bacteria and fungi. Internal Control (IC) (2G) can be added to a PCR amplification reaction mixture to determine whether a test sample inhibits the amplification reaction and may result in false negative results. It can also be added to the sample before extraction to evaluate the extraction efficiency.
Specification
Inclusivity：More than 180 species of Mycoplasma, Spiroplasma and Acholeplasma
Detection Limit：10 CFU/mL or 1 genome copy/μL (Positive cut-off: 95%)
Specificity：No matrix-induced effect across different sample matrices and no cross-reactivity to nearly 30 types of cell lines
Robustness：
Freeze-thaw stability over 5 cycles
Instrument suitability not limited to Thermo, Biorad, Roche, HZSKBio qPCR equipments
Consistent LOC in different matrices
Mycoplasma DNA Detection Kit (2G) contents and storage
| Reagent | Part No. | Quantity | Storage |
| MyqPCR reaction buffer (2G) | NNA035 | 1 bottle | -20℃ |
| MyPrimer & Probe MIX (2G) | NNA039 | 1 bottle | |
| Internal control (IC) (2G)* | NNB004 | 400 μL × 1 tube | -20℃, protect from ligh |
| Positive control (PC) (2G)* | NNC065 | 75 μL × 1 tube | |
| DNA dilution buffer | NND001 | 1.5 mL × 2 tubes | -20℃ |
The kit components can be stored at appropriate conditions for up to 24 months.
Please remember our product information: Mycoplasma DNA Detection Kit (2G) catalog number: 1509841 MycoSHENTEK®.