Catalog No.	PA007126
Product Name	Mouse IgG1 Isotype Control Antibody (Clone: 12B9) | PA007126
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	Recombinant Mouse IgG1 Isotype Control, Mouse IgG1 Negative Control Antibody, mIgG1 Isotype Control
Summary	The in vivo grade recombinant mouse IgG1 isotype control antibody (mIgG1 isotype control) was produced in CHO cells.
Isotype	mouse IgG1, kappa
Applications	an isotype-matched negative control for mouse IgG1 antibody used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry
Immunogen	N/A.
Form Of Antibody	0.2 μM filtered solution of 1x PBS.
Endotoxin	Less than 1 EU/mg of protein as determined by LAL method.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The recombinant mouse IgG1 isotype control and mutants are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 1 month from date of receipt, 2 to 8°C as supplied. 3 months from date of receipt, -20°C to -70°C as supplied.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007126: Recombinant Mouse IgG1 Isotype Control Antibody(Clone: 12B9), In Vivo Grade

The Mouse IgG1 Isotype Control Antibody (12B9) is a must-have for researchers seeking to ensure experimental accuracy in immunological studies. Designed as a negative control, it distinguishes specific antibody binding from non-specific background signals in critical applications like Flow Cytometry, ELISA, Immunohistochemistry (IHC-P, IHC-F), and Western Blot. Available from trusted suppliers like us, this recombinant antibody matches Mouse IgG1 kappa primary antibodies and shows no reactivity to mouse, human, or rat proteins, making it ideal for validating experiments with human PBMCs or mouse splenocytes. With high purity (>95% by SDS-PAGE) and low endotoxin levels (<0.1 EU/µg), it delivers reliable results in both in vivo and in vitro research, encouraging researchers to trust its performance in sensitive assays.

Researchers value tools that streamline workflows and ensure reproducibility, and this antibody excels with its recombinant production in mammalian cells (CHO or HEK293), offering unmatched lot-to-lot consistency compared to hybridoma-derived alternatives. Available in versatile formats—purified, FITC, PE, APC conjugates, and Fc-silenced variants—it supports diverse needs, from flow cytometry isotype control for precise staining to IHC for tissue analysis. Supplied as a 0.2 µm filtered PBS solution (pH 7.2) or lyophilized with stabilizers, it is easy to use and stable, with storage at 2-8°C for short-term or -20°C for long-term, avoiding freeze-thaw cycles. Its ethical production, free from mouse ascites, aligns with modern research standards, making it a compelling choice for labs prioritizing quality and sustainability.

To drive purchasing decisions, the Mouse IgG1 Isotype Control (Clone: 12B9) is rigorously tested for minimal background staining across human and mouse tissues, backed by a 100% satisfaction guarantee and detailed technical data sheets from suppliers like us. Competitively priced (starting at >$36 for 1 mg) and available in flexible sizes (50 µg, 100 µg, 1 mg), it fits various research budgets. Fast shipping and responsive customer support ensure seamless integration into experiments, while its proven performance in applications like flow cytometry and IHC makes it a go-to for researchers seeking reliable results.

Please contact us to ask for a quote for the Fc silenced mouse IgG1 isotype control mutants, mIgG1 isotype control and mutants with Avi-, His-, and Flag-tags, and biotinylated mIgG1 isotype control and mutants. A variety of conjugates (such as dyes and fluorophores) with the mouse IgG1 isotype control and Fc-slient mutants are available.

References of Mouse IgG1 Isotype Control Antibody:

1、Self-Renewal and Toll-like Receptor Signaling Sustain Exhausted Plasmacytoid Dendritic Cells during Chronic Viral Infection.
Macal, M., et al. Immunity. 2018 Apr 17;48(4):730-744.e5. doi: 10.1016/j.immuni.2018.03.020. PMID: 29669251.
“WT mice were treated with 500μg/mouse intraperitoneally (i.p.) with neutralizing antibody (nAb) to IFNAR (clone MAR1-5A3; BioXCell) or Anti-Mouse IgG1 Isotype Control (clone MOPC-21; BioXCell) on days −1 and 0 post-LCMV Cl13-infection. …Livers (or salivary glands for MCMV viral stock titration) were homogenized 3.5 days post-MCMV infection and serially diluted samples were incubated on NIH 3T3 monolayer with rotation for 2hr at 37°C. …We thank Christie Lyn Costanza and Marta Paez-Quinde for help with recruitment of human subjects. …Together, these results indicated that both acute and chronic infections compromised long-term the ability of BM to generate pDCs. …The numbers of CDPs and CD115− progenitors tended to increase at day 5 p.i. but were also reduced by day 10 p.i. in both ARM- and Cl13-infected mice compared to uninfected controls.”

2、Dietary salt promotes neurovascular and cognitive dysfunction through a gut-initiated TH17 response.
Faraco, G., et al. Nat Neurosci. 2018 Feb;21(2):240-249. doi: 10.1038/s41593-017-0059-z. PMID: 29335605.
“A diet rich in salt is linked to an increased risk of cerebrovascular diseases and dementia, but it remains unclear how dietary salt harms the brain. …We report that, in mice, excess dietary salt suppresses resting cerebral blood flow and endothelial function, leading to cognitive impairment. …The effect depends on expansion of TH17 cells in the small intestine, resulting in a marked increase in plasma interleukin-17 (IL-17). …The findings reveal a new gut–brain axis linking dietary habits to cognitive impairment through a gut-initiated adaptive immune response compromising brain function via circulating IL-17. …Thus, the TH17 cell–IL-17 pathway is a putative target to counter the deleterious brain effects induced by dietary salt and other diseases associated with TH17 polarization.”

3、Control of murine cytomegalovirus infection by γδ T cells.
Sell S, et al. PLoS Pathog. 2015 Feb 6;11(2):e1004481. doi: 10.1371/journal.ppat.1004481. PMID: 25658831.
“In addition neutralizing antibodies against IFNγ (clone XMG1.2), IL-17 (clone 17F3) or their isotype controls (rat IgG1 and mouse IgG1 respectively) were administered. …For analysis of whole blood lymphocytes 2 drops of blood were taken from the tail vein in heparinized tubes (Greiner bio-one). …The control of CMV infections relies on multiple and redundant immune effector functions from the innate and the adaptive immune system. …Taken together, the results show that γδ T cells are capable of controlling a primary MCMV infection in the absence of additional cells from the adaptive immune system. …In addition we performed infections with TCRα-/- mice that completely lack αβ T cells.”

4、Adaptive Immunity to Leukemia Is Inhibited by Cross-Reactive Induced Regulatory T Cells.
Manlove LS, et al. J Immunol. 2015 Oct 15;195(8):4028-37. doi: 10.4049/jimmunol.1501291. PMID: 26378075.
“BCR-ABL+ acute lymphoblastic leukemia patients have transient responses to current therapies. …However, the fusion of BCR to ABL generates a potential leukemia-specific Ag that could be a target for immunotherapy. …To address how BCR-ABL+ leukemia escapes immune surveillance, we developed a peptide: MHC class II tetramer that labels endogenous BCR-ABL–specific CD4+ T cells. …The small number of naive BCR-ABL–specific T cells was due to negative selection in the thymus, which depleted BCR-ABL–specific T cells. …Despite this cross-reactivity, the remaining population of BCR-ABL reactive T cells proliferated upon immunization with the BCR-ABL fusion peptide and adjuvant. …Treg cells were critical for leukemia progression in C57BL/6 mice, as transient Treg cell ablation led to extended survival of leukemic mice.”

5、The degree of CD4+ T cell autoreactivity determines cellular pathways underlying inflammatory arthritis.
Aitken, M., Rankin, A. L., Garcia, V., Kropf, E., Erikson, J., Garlick, D. S., Caton, A. J., et al. J Immunol. 2014 Apr 1;192(7):3043-56. doi: 10.4049/jimmunol.1302528. Epub 2014 Mar 3. PMID: 24591372.
“Although therapies targeting distinct cellular pathways (e.g., anticytokine versus anti–B cell therapy) have been found to be an effective strategy for at least some patients with inflammatory arthritis, the mechanisms that determine which pathways promote arthritis development are poorly understood. …We have used a transgenic mouse model to examine how variations in the CD4+ T cell response to a surrogate self-peptide can affect the cellular pathways that are required for arthritis development. …By contrast, arthritis develops with a significant female bias in the context of a more weakly autoreactive CD4+ T cell response, and B cells play a prominent role in disease pathogenesis. …In this setting of lower CD4+ T cell autoreactivity, B cells promote the formation of autoreactive CD4+ effector T cells (including Th17 cells), and IL-17 is required for arthritis development. …These studies show that the degree of CD4+ T cell reactivity for a self-peptide can play a prominent role in determining whether distinct cellular pathways can be targeted to prevent the development of inflammatory arthritis.”

6、Smac mimetics and innate immune stimuli synergize to promote tumor death.
Beug ST, et al. Nat Biotechnol. 2014 Feb;32(2):182-90. doi: 10.1038/nbt.2806. PMID: 24463573.
“Smac mimetic compounds (SMC), a class of drugs that sensitize cells to apoptosis by counteracting the activity of inhibitor of apoptosis (IAP) proteins, have proven safe in phase 1 clinical trials in cancer patients. …However, because SMCs act by enabling transduction of pro-apoptotic signals, SMC monotherapy may be efficacious only in the subset of patients whose tumors produce large quantities of death-inducing proteins such as inflammatory cytokines. …Therefore, we reasoned that SMCs would synergize with agents that stimulate a potent yet safe “cytokine storm.” …Here we show that oncolytic viruses and adjuvants such as poly(I:C) and CpG induce bystander death of cancer cells treated with SMCs that is mediated by interferon beta (IFN-β), tumor necrosis factor alpha (TNF-α) and/or TNF-related apoptosis-inducing ligand (TRAIL). …This combinatorial treatment resulted in tumor regression and extended survival in two mouse models of cancer.”

7、FoxP3+ regulatory T cells promote influenza-specific Tfh responses by controlling IL-2 availability.
León B, et al. Nat Commun. 2014 Mar 17;5:3495. doi: 10.1038/ncomms4495. PMID: 24633065.
“Thus, the factors that control the physiological availability of IL-2 are likely to regulate Tfh development and the ensuing B-cell response. …In fact, mice with natural or targeted mutations in FoxP3 fail to develop Tregs and spontaneously accumulate autoreactive Tfh and GC cells. …Despite their reputation as suppressor cells, Tregs may also promote antigen-specific B-cell responses under some circumstances. …Here, we show that Treg depletion compromises influenza-specific GC responses. …The loss of Tfh following Treg depletion is not due to a precursor–progeny relationship between FoxP3-expressing cells and Tfh or the lack of TGFβ.”

8、Signaling via the IL-20 receptor inhibits cutaneous production of IL-1β and IL-17A to promote infection with methicillin-resistant Staphylococcus aureus.
Fontecilla NM, Valdez PA, Vithayathil PJ, Naik S, Belkaid Y, Ouyang W, Datta SK, et al. Nat Immunol. 2013 Aug;14(8):804-11. doi: 10.1038/ni.2637. Epub 2013 Jun 23. PMID: 23793061.
“Staphylococcus aureus causes most infections of human skin and soft tissue and is a major infectious cause of mortality. …Host defense mechanisms against S. aureus are incompletely understood. …Interleukin 19 (IL-19), IL-20 and IL-24 signal through type I and type II IL-20 receptors and are associated with inflammatory skin diseases such as psoriasis and atopic dermatitis. …We found here that those cytokines promoted cutaneous infection with S. aureus in mice by downregulating IL-1β- and IL-17A-dependent pathways. …Our findings identify an immunosuppressive role for IL-19, IL-20 and IL-24 during infection that could be therapeutically targeted to alter susceptibility to infection.”

9、Programmed cell death ligand 2 regulates TH9 differentiation and induction of chronic airway hyperreactivity.
Maazi H, Speak AO, Szely N, Lombardi V, Khoo B, Geryak S, Lam J, Soroosh P, Van Snick J, Akbari O, et al. J Allergy Clin Immunol. 2013 Apr;131(4):1048-57, 1057.e1-2. doi: 10.1016/j.jaci.2012.09.027. PMID: 23174661.
“Purified CD4+DO11.10+ T cells were cultured for 3 days in round-bottom 96-well plates (1 × 105 cells per well) with ovalbumin (OVA)–loaded (OVA peptide 323-339) bone marrow–derived dendritic cells (DCs; ratio, 1:32) from wild-type (WT) or PD-L2−/− mice in the presence of 10 μg/mL anti–IFN-γ antibodies (clone XMG1.2; BioXcell, West Lebanon, NH), 2 ng/mL rTGF-β1 (eBioscience) and 10 ng/mL rIL-4 (eBioscience) with or without 10 μg/mL anti–PD-1 blocking antibodies (clone J43, eBioscience), anti–PD-L2 blocking antibodies (clone mAb 3.2, a gift of Dr. Gordon Freeman, Harvard Medical School25), or IgG1 isotype control antibodies (IgG1, MOPC-21, BioXCell). …Several groups have reported that IL-9 production is enhanced in the lungs of human patients with chronic asthma. …Therefore we have developed a novel protocol to induce chronic AHR in mice through intranasal exposure to doses of A fumigatus lysates for more than 6 weeks. …These results suggest that although CD4+ T cells producing TH2 cytokines are present in both the lungs and the LNs of mice during the acute and chronic phases of AHR, the development of TH9 cells is restricted to the lungs. …The current hypothesis is that TH9 cells develop from TH2 cells, and costimulatory molecules have been shown to influence T-cell polarization. …Recently, we reported that PD-L2 has an important role in the regulation of acute AHR in mice.”

10、IL-17A and IL-2-expanded regulatory T cells cooperate to inhibit Th1-mediated rejection of MHC II disparate skin grafts.
Vokaer B, et al. PLoS One. 2013 Oct 11;8(10):e76040. doi: 10.1371/journal.pone.0076040. PMID: 24146810.
“Several evidences suggest that regulatory T cells (Treg) promote Th17 differentiation. …We and others recently identified a role for IL-17A producing CD4+ and CD8+ T cells in the allograft rejection process. …This probably reflects the redundant pathways of allograft rejection involving numerous alloreactive effector cells belonging to T helper (Th)1, Th2 and CD8+ T cells. …Mice were anaesthetized with a mixture of xylazine (Rompun) 5% and ketamine 10% in phosphate-buffered saline (PBS). …For in vitro suppression assay, WT CD4+ T cells were isolated using CD4 negative isolation kit from Miltenyi, and used as responder cells.”

For more references about Mouse IgG1 Isotype Control Antibody please contact our scientific support team with message@sydlabs.com.

Other in vivo grade Recombinant IgG Isotype Control Antibodies and Mutants:
Recombinant Human IgG1 Isotype Control Antibody and Mutants, In vivo Grade
Recombinant Human IgG2 Isotype Control Antibody, In vivo Grade
Recombinant Human IgG3 Isotype Control Antibody, In vivo Grade
Recombinant Human IgG4-S228P Isotype Control Antibody and Mutants, In vivo Grade
Recombinant Mouse IgG1 Isotype Control Antibody and Mutants, In vivo Grade
Recombinant Mouse IgG2a Isotype Control Antibody and Mutants, In vivo Grade
Recombinant Mouse IgG2b Isotype Control Antibody and Mutants, In vivo Grade
Recombinant Mouse IgG2c Isotype Control Antibody and Mutants, In vivo Grade
Recombinant Mouse IgG3 Isotype Control Antibody, In vivo Grade
Recombinant Rat IgG1 Isotype Control Antibody, In vivo Grade
Recombinant Rat IgG2a Isotype Control Antibody, In vivo Grade
Recombinant Rat IgG2b Isotype Control Antibody, In vivo Grade
Recombinant Rat IgG2c Isotype Control Antibody, In vivo Grade
Recombinant Hamster IgG1 Isotype Control Antibody, In vivo Grade
Recombinant Hamster IgG2 Isotype Control Antibody, In vivo Grade

In vivo Grade Recombinant IgG Fc Proteins:
Recombinant Human IgG1 Fc Protein (hIgG1), In vivo Grade
Recombinant Human IgG2 Fc Protein (hIgG2), In vivo Grade
Recombinant Human IgG4 Fc Protein (hIgG4), In vivo Grade
Recombinant Mouse IgG1 Fc Protein (mIgG1), In vivo Grade
Recombinant Mouse IgG2a Fc Protein (mIgG2a), In vivo Grade
Recombinant Mouse IgG2b Fc Protein (mIgG2b), In vivo Grade
Recombinant Rat IgG2a Fc Protein (rtIgG2a), In vivo Grade
Recombinant Rat IgG2b Fc Protein (rtIgG2b), In vivo Grade
Recombinant Llama IgG2b Fc Protein (lIgG2b), In vivo Grade
Recombinant Rabbit IgG Fc Protein (rIgG), In vivo Grade

Fc ELISA Kits and Reagents:
Human Fc ELISA Kit
Mouse Fc ELISA Kit
Human Fc ELISA Reagent Kit
Mouse Fc ELISA Reagent Kit

Mouse IgG1 Isotype Control (12B9) from: In Vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody PA007126: Syd Labs

No more offers for this product!