Catalog No.	BP4111
Product Name	Mouse IgG1 Fc Protein | BP4111
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	Mouse Fc protein (IgG1), Mouse IgG1 Fc region, Recombinant mIgG1 Fc Protein
Summary	The in vivo grade recombinant mouse IgG1 Fc protein (mIgG1 Fc, Val 98 - Lys 324 of the constant region of the IgG1 heavy chain, Accession # P01868-1) was produced in mammalian cells and purified by protein G. The mIgG1 Fc protein includes the CH2 (111-217) and CH3 (218-324) domains of the heavy chain constant region as well as the hinge region (98-110) before the CH2 domain. The C102S version of the mIgG1 Fc protein is also available. The C102S substitution prevents improper cysteine bond formation in the absence of a light chain.
Form Of Antibody	0.2 μM filtered solution of 1x PBS.
Endotoxin	Less than 1 EU/mg of protein as determined by LAL method.
Purity	>95% by SDS-PAGE under reducing conditions
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

BP4111: Recombinant Mouse IgG1 Fc Protein, In Vivo Grade

Background

In vivo grade recombinant natural and engineered mouse IgG1 Fc proteins (mIgG1 Fc), and recombinant mouse IgG1 isotype control antibody and mutants are available. Biotinylated mIgG1 Fc, and mIgG1 Fc with Avi-, His-, and Flag-tags are also available. Please contact us to ask for a quote for the engineered mouse IgG1 Fc proteins, biotinylated mIgG1 Fc, and mIgG1 Fc with Avi-, His-, and Flag-tags. The recombinant mouse IgG1 Fc protein and mutants can be used as the isotype control of the mIgG1 antibodies and the mIgG1 Fc fusion proteins in various in vitro and in vivo experiments.

The T252M substitution of the mIgG1 Fc protein can significantly increase the affinity to protein A.

The in vivo grade recombinant mouse IgG1 Fc protein (mIgG1 Fc, Val 98 – Lys 324 of the constant region of the IgG1 heavy chain, Accession # P01868-1) was produced in mammalian cells and purified by protein G. The mIgG1 Fc protein includes the CH2 (111-217) and CH3 (218-324) domains of the heavy chain constant region as well as the hinge region (98-110) before the CH2 domain. The C102S version of the mIgG1 Fc protein is also available. The C102S substitution prevents improper cysteine bond formation in the absence of a light chain.

References of Mouse IgG1 Fc Protein:

1、Chimeric Fusion between Clostridium Ramosum IgA Protease and IgG Fc Provides Long-Lasting Clearance of IgA Deposits in Mouse Models of IgA Nephropathy.
Xie, X., et al. J Am Soc Nephrol. 2022 May;33(5):918-935. doi: 10.1681/ASN.2021030372. PMID: 35172987.
“To extend the t1/2 in blood, we fused AK183 to the Fc segment of human IgG1. …First, we discovered an autocleavage activity of AK183 that separates the N-terminal protease from its C-terminal autotransporter β domain. …The combined actions of Fc-AK183-mediated cleavage and subsequent renal clearance of IgA resulted in a lasting obliteration of blood IgA, as demonstrated in a human IgA-injection model and in a humanized α1KI transgenic model. …Fc-AK183 was also able to remove chronic IgA and associated complement C3 deposits in the glomerulus. …IgA nephropathy is a common primary glomerulonephritis caused by mesangial deposition of poly-IgA complexes.”

2、Therapeutic benefits of recombinant alpha1-antitrypsin IgG1 Fc-fusion protein in experimental emphysema.
Takeda, K., et al. Respir Res. 2021 Jul 16;22(1):207. doi: 10.1186/s12931-021-01784-y. PMID: 34271910.
“Mice received AAT-Fc, pAAT, IgG1-Fc or vehicle 21 days after PPE instillation and the effects in the lungs were analyzed 32 days after PPE (AAT Fc PPE day 32, pAAT PPE day 32, IgG1-Fc PPE day 32, and PBS PPE day 32, respectively). …Lung function testing in CS-exposed mice revealed significant reductions in Cst following treatment with AAT-Fc (14% increase) but not following pAAT or IgG1-Fc treatment which were similar to vehicle treatment (Fig. 3B). …In all aspects, the effects of AAT-Fc were greater than those seen with pAAT or control, IgG1-Fc. The benefits of AAT-Fc treatment were also shown in CS-exposed mice with improvements in airspace enlargement. …Spleen MNCs isolated from PPE-treated mice and exposed to EP produced IFNγ and IL-17. In these studies, the inhibitory effects of AAT-Fc on cytokine production were shown to reside on EP primed-DCs when co-cultured with CD4+ T cells. …However, studies revealed only modest effects of this therapy; this treatment slowed progression of emphysema as measured by lung density with computed tomography or total lung capacity, but not when measured by functional residual capacity.”

3、Modulation of Neutrophil Function by Recombinant Human IgG1 Fc Hexamer in the Endogenous K/BxN Mouse Model of Rheumatoid Arthritis.
Almizraq, R. J., et al. Pharmacology. 2023 Feb;108(2):176-187. doi: 10.1159/000528780. PMID: 36696888.
“In this study, due to its high avidity for binding to FcγRs, we investigated the potential anti-inflammatory effect of a recombinant IgG1 Fc hexamer (rFc-µTP-L309C) on neutrophils in the K/BxN mouse model of endogenously generated chronic arthritis. …It was produced by fusing the 18-aa IgM tailpiece to the C-terminus of a variant human IgG1 Fc with a point mutation at position 309, and this point mutation stabilizes the hexamer through the formation of disulfide bridges. …Recombinant IgG1 Fc hexamer (rFc-µTP-L309C) was obtained from CSL Behring AG, Switzerland. …Treatment with rFc-µTP-L309C, but not HSA, was able to significantly ameliorate the arthritis in the K/BxN mice. …In the endogenous K/BxN mouse model of rheumatoid arthritis, amelioration can be explained in part by inhibition of neutrophil infiltration into the joints as well as inhibition of IL-1β production.”

For more references about Mouse IgG1 Fc Protein please contact our scientific support team with message@sydlabs.com.

Other Recombinant IgG Fc Proteins:
Recombinant Human IgG1 Fc Protein (hIgG1), In vivo Grade
Recombinant Human IgG2 Fc Protein (hIgG2), In vivo Grade
Recombinant Human IgG4 Fc Protein (hIgG4), In vivo Grade
Recombinant Mouse IgG1 Fc Protein (mIgG1), In vivo Grade
Recombinant Mouse IgG2a Fc Protein (mIgG2a), In vivo Grade
Recombinant Mouse IgG2b Fc Protein (mIgG2b), In vivo Grade
Recombinant Mouse IgG2c Fc Protein (mIgG2c), In vivo Grade
Recombinant Mouse IgG3 Fc Protein (mIgG3), In vivo Grade
Recombinant Rat IgG2a Fc Protein (rtIgG2a), In vivo Grade
Recombinant Rat IgG2b Fc Protein (rtIgG2b), In vivo Grade
Recombinant Llama IgG2b Fc Protein (lIgG2b), In vivo Grade
Recombinant Rabbit IgG Fc Protein (rIgG), In vivo Grade

Recombinant IgG Reference Antibodies:
Recombinant Human IgG1 Isotype Control Antibody and Mutants, In vivo Grade
Recombinant Human IgG2 Isotype Control Antibody, In vivo Grade
Recombinant Human IgG3 Isotype Control Antibody, In vivo Grade
Recombinant Human IgG4-S228P Isotype Control Antibody and Mutants, In vivo Grade
Recombinant Mouse IgG1 Isotype Control Antibody and Mutants, In vivo Grade
Recombinant Mouse IgG2a Isotype Control Antibody and Mutants, In vivo Grade
Recombinant Mouse IgG2b Isotype Control Antibody and Mutants, In vivo Grade
Recombinant Mouse IgG2c Isotype Control Antibody and Mutants, In vivo Grade
Recombinant Mouse IgG3 Isotype Control Antibody, In vivo Grade
Recombinant Rat IgG1 Isotype Control Antibody, In vivo Grade
Recombinant Rat IgG2a Isotype Control Antibody, In vivo Grade
Recombinant Rat IgG2b Isotype Control Antibody, In vivo Grade
Recombinant Rat IgG2c Isotype Control Antibody, In vivo Grade

Fc ELISA Kits and Reagents:
Human Fc ELISA Kit
Mouse Fc ELISA Kit
Human Fc ELISA Reagent Kit
Mouse Fc ELISA Reagent Kit

Mouse IgG1 Fc Protein from: In Vivo Grade Recombinant Mouse IgG1 Fc Protein: BP4111 Syd Labs

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