Biotinylated Anti-human CD34 Antibody (My10, Human IgG1 Fc Silent Kappa)
$150.00 – $300.00
Biotinylated recombinant anti-human CD34 monoclonal antibody (Clone My10), engineered with a Human IgG1 Fc-silent domain to minimize non-specific background binding to Fc receptors during cell analysis. Features site-directed biotinylation via a C-terminal Avi tag on the heavy chain, with variable regions derived from anti-human CD34 clone (My10) and expressed in mammalian cells.
- Conjugation & Purity: Biotinylated (Site-directed via C-terminal Avi-tag), > 95% by SDS-PAGE
- Applications: Flow Cytometry (FC) via Streptavidin Conjugates, Cell Isolation, Hematopoietic Stem/Progenitor Cell Gating
- Alternative Formats: Unconjugated human IgG1 Fc-silent, mouse IgG1, and other recombinant versions of clone My10 are also available at Syd Labs and Ushelf.
- Details & Specifications
- References
| Catalog No. | PA007757.h1Fs-b |
|---|---|
| Product Name | Biotinylated Recombinant Anti-human CD34 Monoclonal Antibody (Clone: My10), Human IgG1 Fc Silent Kappa |
| Supplier Name | Syd Labs, Inc. |
| Brand Name | Syd Labs |
| Clone | My10 |
| Isotype | Human IgG1 Fc Silent Kappa. |
| Source/Host | The site-directed biotinylated anti-human CD34 monoclonal antibody (clone: My10) was produced in mammalian cells. |
| Specificity/Sensitivity | The site-directed biotinylated recombinant My10 antibody specifically binds to human CD34. |
| Applications | Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads. |
| Form Of Antibody | 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives. |
| Conjugate | Biotin-conjugated. |
| Purity | >95% by SDS-PAGE under reducing conditions. |
| Shipping | The My10 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied. |
| Note | Recombinant mouse IgG1 isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator. |
| Order Offline | Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card. |
Description
PA007757.h1Fs-b: Biotinylated Recombinant Anti-human CD34 Monoclonal Antibody (Clone: My10), Human IgG1 Fc Silent Kappa
Recombinant human IgG1 monoclonal antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
References of Anti-Human CD34 Monoclonal Antibody (Clone: My10):
Maleki LA, et al. Cell J. 2014 Spring;15(4):292-300. PMID: 24611141
“The My10 monoclonal antibody, recognizing CD34, was tested for specificity via ELISA and Western blotting. …Subisotyping confirmed its immunoglobulin class, and it enabled identification and enrichment of hematopoietic stem/progenitor cells.”
Civin CI, et al. Blood. 1987 Nov;70(5):1316-23. PMID: 3489673
“Two-color cell sorting with My10 and HLA-DR antibodies enriched hematopoietic progenitors in bone marrow, with the My10-positive, HLA-DR-positive fraction containing nearly all CFU-GM and BFU-E. …Flow cytometry and progenitor assays confirmed high purity and enrichment of progenitor cells using My10.”
Queen C, et al. Cancer Res. 1992 Dec 15;52(24 Suppl):6761s-6767s. PMID: 1458463
“The My10 anti-CD34 antibody was humanized via CDR grafting, retaining binding affinity to CD34+ KG1 cells in flow cytometry. …Comparative analysis showed similar reactivity between parental and humanized versions, with molecular modeling guiding framework region selection for humanization.”
Kasahara N, et al. Hum Gene Ther. 1999 Mar 20;10(5):937-46. PMID: 10094198
“The My10 scFv, expressed on retroviral vectors, targeted CD34+ KG1 cells, with flow cytometry confirming specific binding. …While targeting improved transduction specificity, CD34 engagement did not enhance virus-cell fusion, as assessed in post-binding assays.”
5. My10, a new myeloid cell antigen recognized by a monoclonal antibody
Civin CI, et al. J Exp Med. 1983 Oct 1;158(4):1125-36. PMID: 6606698
“The My10 monoclonal antibody recognized a 115-kD myeloid antigen on blasts and progenitors, absent on mature granulocytes, as shown by flow cytometry. …Sorting with My10 enriched CFU-GM, and immunoprecipitation confirmed the antigen’s molecular weight, establishing its utility in myeloid cell identification.”
For more technical references or data sheets regarding the Recombinant Anti-Human CD34 Monoclonal Antibody (Clone: My10) formats, please contact our scientific support team at message@sydlabs.com or explore options via Syd Labs.
Syd Labs provides the following recombinant anti-human CD34 monoclonal antibodies for flow cytometry:
Recombinant anti-human CD34 monoclonal antibodies (clone QBEnd/10) for flow cytometry
Recombinant anti-human CD34 monoclonal antibodies (clone My10) for flow cytometry
Recombinant anti-human CD34 monoclonal antibodies (clone 581) for flow cytometry
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