Biotinylated Anti-human CD32 Antibody (IV.3, Human IgG1 Fc Silent Kappa)
$125.00 – $250.00
Biotinylated recombinant anti-human CD32 / FcγRII monoclonal antibody (Clone IV.3), engineered with a Human IgG1 Fc-silent domain to minimize non-specific background binding to Fc receptors during cell analysis. Features site-directed biotinylation via a C-terminal Avi tag on the heavy chain, with variable regions derived from anti-human CD32 clone (IV.3) and expressed in mammalian cells.
- Conjugation & Purity: Biotinylated (Site-directed via C-terminal Avi-tag), > 95% by SDS-PAGE
- Applications: Flow Cytometry (FC) via Streptavidin Conjugates, Fc Receptor Blocking, Cell Isolation
- Alternative Formats: Unconjugated human IgG1 Fc-silent, mouse IgG1, and other recombinant versions of clone IV.3 are also available at Syd Labs and Ushelf.
- Details & Specifications
- References
| Catalog No. | PA007752.h1Fs-b |
|---|---|
| Product Name | Biotinylated Recombinant Anti-human CD32 Monoclonal Antibody (Clone: IV.3), Human IgG1 Fc Silent Kappa |
| Supplier Name | Syd Labs, Inc. |
| Brand Name | Syd Labs |
| Clone | IV.3. |
| Isotype | Human IgG1 Fc Silent Kappa. |
| Source/Host | The site-directed biotinylated anti-human CD32 monoclonal antibody (clone: IV.3) was produced in mammalian cells. |
| Specificity/Sensitivity | The site-directed biotinylated recombinant IV.3 antibody specifically binds to human CD32. |
| Applications | Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads. |
| Form Of Antibody | 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives. |
| Conjugate | Biotin-conjugated. |
| Purity | >95% by SDS-PAGE under reducing conditions. |
| Shipping | The IV.3 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied. |
| Note | The human CD32 Fc blocking antibody (IV.3 Fc blocking antibody) is an anti-human Fc receptor antibody for human FcR blocking reagent. Various isotypes are available for certain applications. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator. |
| Order Offline | Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card. |
Description
PA007752.h1Fs-b: Biotinylated Recombinant Anti-human CD32 Monoclonal Antibody (Clone: IV.3), Human IgG1 Fc Silent Kappa
Recombinant human IgG1 monoclonal antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
The recombinant IV.3 antibody binds to human CD32 (FcγRII or FCGR2), a cluster of differentiation molecule found on the surface of a variety of immune cells. CD32 is a surface receptor glycoprotein belonging to the Ig gene superfamily, has a low-affinity for the Fc region of IgG antibodies in monomeric form, but high affinity for IgG immune complexes. CD32 has two major functions: cellular response regulation, and the uptake of immune complexes. CD32 consists of Fc receptors FcγRIIa (CD32a), FcγRIIb (CD32b), and FcγRIIc (CD32c).
The most popular human Fc blocking reagents include:
Anti-human CD16 (clone 3G8), and CD32 (Clone IV.3): for flow cytometry, immunohistochemistry (IHC), and immunoprecipitation (IP). It is highly specific for FcγRII (CD32) and FcγRIII (CD16), and reduces background staining; it may not block all Fc receptors. Clones 10.1, and 22 or H22 are the most widely used anti-human FcγRI (CD64) clone for Fc blocking in flow cytometry and functional assays. It prevents non-specific Fc receptor binding on monocytes, macrophages, and dendritic cells, improving antibody specificity.
Purified human IgGs or mixes of human isotype controls: for general Fc blocking in IHC and immunofluorescence (IF). It is cheap and easy to use but it is less specific, and may even introduce unwanted immunoglobulins. Syd Labs supply various human IgG isotype controls.
Normal mouse serum, purified mouse IgG or isotype controls: for flow cytometry. It can serve as a control to block Fc receptors but it is non-specific for Fc receptors. It is cheap and easy to use but it is less specific, and may even introduce unwanted immunoglobulins. Our recombinant mouse IgG antibody mixes are affordable; the ratio of various IgGs is optimized and adjustable without any unwanted immunoglobulins.
Bovine Serum Albumin (BSA) or FBS: for general blocking in Western Blot (WB) and IHC. They are readily available to reduce non-specific binding but less effective at Fc receptor blocking.
Commercial Fc blocking solutions: for flow cytometry, IHC, and functional assays. They are normally pre-optimized and highly effective but more expensive than DIY solutions.
Best choice of human Fc blocking reagents based on your application are:
For flow cytometry: anti-human CD16 (Clone 3G8), CD32 (Clone IV.3), and CD64 (Clone 10.1, and 22 or H22).
For Immunohistochemistry (IHC) and Immunofluorescence (IF): Normal mouse serum, purified mouse or human IgGs or isotype controls or commercial Fc blocking kits. Our recombinant mouse IgG antibody mixes and recombinant human IgG isotype controls are affordable; the ratio of various IgGs is optimized and adjustable without any unwanted immunoglobulins.
For Western Blot and ELISA: BSA or FBS.
For functional assays (e.g., blocking Fc-mediated effects): Commercial Fc blocking reagents.
Syd Labs provides the following in vivo grade recombinant anti-human CD16, CD32, and CD64 monoclonal antibodies:
Recombinant Anti-human CD16 monoclonal antibody (Clone: 3G8)
Recombinant Anti-human CD32 monoclonal antibody (Clone: IV.3)
Recombinant Anti-human CD64 monoclonal antibody (Clone: H22)
Syd Labs provides the following in vivo grade recombinant anti-mouse CD16, CD32, and CD64 monoclonal antibodies:
Recombinant Anti-mouse CD16/CD32 monoclonal antibody (Clone: 2.4G2)
Syd Labs provides the following recombinant anti-human CD16, CD32, and CD64 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD16 monoclonal antibody (Clone: 3G8) for flow cytometry
Recombinant Anti-human CD32 monoclonal antibody (Clone: IV.3) for flow cytometry
Recombinant Anti-human CD64 monoclonal antibody (Clone: H22) for flow cytometry
Syd Labs provides the following recombinant anti-mouse CD16, CD32, and CD64 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD16/CD32 monoclonal antibody (Clone: 2.4G2) for flow cytometry
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