Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8) PA007331.h1Fs Syd Labs

Recombinant Anti-human CD16Monoclonal Antibody- (Clone 3G8)

Anti-human CD16 Antibody for Flow Cytometry | PA007331.h1Fs

Name: Anti-human CD16 Antibody for Flow Cytometry
Brand: Syd Labs
SKU: PA007331.h1Fs
Price: 40-80 USD
Availability: InStock
Rating: 5 (1 reviews)

$65.00 – $130.00

Recombinant mouse IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.

Details & Specifications
References

Catalog No.	PA007331.h1Fs
Product Name	Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8), Human IgG1 Fc Silent Kappa
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	cluster of differentiation 16, Fc gamma RIIIA and Fc gamma RIIIB
Clone	3G8.
Isotype	Mouse IgG1 kappa.
Source/Host	The recombinant anti-human CD16 monoclonal antibody (clone: 3G8) for Flow Cytometry was produced in mammalian cells.
Specificity/Sensitivity	The recombinant 3G8 antibody for Flow Cytometry specifically binds to human and non-human primate CD16.
Applications	Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form Of Antibody	0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Conjugate	Non-conjugated.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8) for Flow Cytometry is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
Note	Recombinant mouse IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007331.h1Fs: Recombinant Anti-HA-Tag Monoclonal Antibody (Clone: 3G8) for Flow Cytometry, Human IgG1 Fc Silent Kappa, In vivo Grade

The recombinant 3G8 antibody binds to human and non-human primate CD16 or FcγRIII, a cluster of differentiation molecule found on the surface of natural killer cells, neutrophils, monocytes, macrophages, and certain T cells. CD16 consists of Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). The most well-researched membrane receptor implicated in triggering lysis by NK cells, CD16 is a molecule of the immunoglobulin superfamily (IgSF) involved in antibody-dependent cellular cytotoxicity (ADCC). It can be used to isolate populations of specific immune cells through fluorescent-activated cell sorting (FACS) or magnetic-activated cell sorting, using antibodies directed towards CD16.

References of Anti-Human CD16 Monoclonal Antibody (Clone: 3G8)

1. Comparison of the different anti-CD16 antibody clones in the activation and expansion of peripheral blood NK cells.
Kwon, et al.PLoS One. 2023 Jun 9;18(6):e0286915. doi: 10.1371/journal.pone.0286915. PMID: 37294741; PMCID: PMC10258201
“Natural killer (NK) cells play an essential role in cancer immunotherapy, and their activation often relies on CD16-mediated signaling pathways. … In this study, we systematically compared the downstream functional outcomes of using different anti-CD16 antibody clones, including 3G8, DJ130c, and KD1, on human peripheral blood NK cells. … Our results show that clone 3G8 induced the most robust NK cell proliferation, degranulation, and inflammatory cytokine secretion compared to other candidate clones.”

2. Factor VIII moiety of recombinant Factor VIII Fc fusion protein impacts Fc effector function and CD16+ NK cell activation.
Ochiel, et al.Front Immunol. 2024 Apr 9;15:1356391. doi: 10.3389/fimmu.2024.1356391. PMID: 38711484; PMCID: PMC11035769
“Recombinant Factor VIII Fc fusion proteins (rFVIIIFc) are widely used therapeutic agents, yet the impact of the large FVIII moiety on Fc-mediated effector functions remains poorly understood. … Here, we investigated how the steric presence of the FVIII moiety modulates Fc binding to FcγRIIIa (CD16) and subsequent natural killer (NK) cell activation. … Using the CD16-blocking monoclonal antibody clone 3G8, we confirmed that rFVIIIFc interacts directly with CD16+ NK cells to trigger downstream intracellular signaling and effector cytokine release.”

3. Multiplex interrogation of the NK cell signalome reveals global downregulation of CD16 signaling during lentivirus infection through an IL-18/ADAM17-dependent mechanism.
Sachan, et al.PLoS Pathog. 2023 Sep 11;19(9):e1011617. doi: 10.1371/journal.ppat.1011617. PMID: 37695786; PMCID: PMC10503717
“Chronic lentivirus infections severely impair natural killer (NK) cell functionality, yet the precise intracellular signaling defects remain incompletely mapped. … We utilized single-cell multiplexed signaling assays to profile downstream targets of the CD16 receptor during active viral infection. … Our findings demonstrate that an IL-18 and ADAM17-dependent pathway drives the shedding of CD16, which was validated using clone 3G8 to track and block functional surface CD16 expression.”

4. NK Cytotoxicity Mediated by NK-92 Cell Lines Expressing Combinations of Two Allelic Variants for FCGR3.
Gomez-Milla, et al.Int J Mol Sci. 2024 Jul 12;25(14):7621. doi: 10.3390/ijms25147621. PMID: 39062864; PMCID: PMC11270249
“The low-affinity IgG receptor FcγRIIIa (CD16) displays genetic polymorphisms, particularly the F158V variation, which influences antibody binding affinity and clinical responses to therapeutic antibodies. … To dissect these differences, we engineered NK-92 cell lines to express single or combined allelic variants of the FCGR3A gene. … Functional characterization using the anti-CD16 monoclonal antibody clone 3G8 demonstrated distinct differences in receptor trigger thresholds and downstream killing dynamics between the high-affinity and low-affinity variants.”

5. Complement activation induces excessive T cell cytotoxicity in severe COVID-19.
Georg, et al.Cell. 2022 Jan 20;185(2):292-312.e25. doi: 10.1016/j.cell.2021.12.019. PMID: 34968393; PMCID: PMC8712270
“Severe COVID-19 is characterized by profound immune dysregulation, hyperinflammation, and progressive lung damage. … We discovered that complement activation in severe patients drives ectopic upregulation of the low-affinity IgG receptor CD16 (FcγRIII) on T cells, rendering them highly cytotoxic. … Blockade of this aberrant CD16 expression on T cells using the 3G8 monoclonal antibody significantly reduced immune-complex-mediated vascular injury, highlighting CD16 as a potential therapeutic target.”

For more technical references or data sheets regarding the Recombinant Anti-Human CD16 Monoclonal Antibody (Clone: 3G8) formats, please contact our scientific support team at message@sydlabs.com.

Syd Labs provides the following recombinant anti-human CD16 monoclonal antibodies for flow cytometry:

Syd Labs provides the following in vivo grade recombinant anti-human CD16, CD32, and CD64 monoclonal antibodies:

Recombinant Anti-human CD16 monoclonal antibody (Clone: 3G8)
Recombinant Anti-human CD32 monoclonal antibody (Clone: IV.3)
Recombinant Anti-human CD64 monoclonal antibody (Clone: H22)

Syd Labs provides the following in vivo grade recombinant anti-mouse CD16, CD32, and CD64 monoclonal antibodies:

Recombinant Anti-mouse CD16/CD32 monoclonal antibody (Clone: 2.4G2)

Syd Labs provides the following recombinant anti-human CD16, CD32, and CD64 monoclonal antibodies for flow cytometry:

Recombinant Anti-human CD16 monoclonal antibody (Clone: 3G8) for flow cytometry
Recombinant Anti-human CD32 monoclonal antibody (Clone: IV.3) for flow cytometry
Recombinant Anti-human CD64 monoclonal antibody (Clone: H22) for flow cytometry

Anti-human CD16 (3G8) for Flow Cytometry from: Anti-human CD16 Monoclonal Antibody (Clone: 3G8): PA007331.h1Fs Syd Labs

Ship from Country	USA
Size	200 ug 50 ug Clear

Anti-human CD16 Antibody for Flow Cytometry | PA007331.h1Fs

Anti-human CD16 Antibody for Flow Cytometry | PA007331.h1Fs