Biotinylated Anti-human CD34 Antibody (581, Human IgG1 Fc Silent Kappa)
$100.00 – $200.00
Biotinylated recombinant anti-human CD34 monoclonal antibody (Clone 581), engineered with a Human IgG1 Fc-silent domain to minimize non-specific background binding to Fc receptors during cell analysis. Features site-directed biotinylation via a C-terminal Avi tag on the heavy chain, with variable regions derived from anti-human CD34 clone (581) and expressed in mammalian cells.
- Conjugation & Purity: Biotinylated (Site-directed via C-terminal Avi-tag), > 95% by SDS-PAGE
- Applications: Flow Cytometry (FC) via Streptavidin Conjugates, Cell Isolation, Hematopoietic Stem Cell Gating
- Alternative Formats: Unconjugated human IgG1 Fc-silent, mouse IgG1, and other recombinant versions of clone 581 are also available at Syd Labs and Ushelf.
- Details & Specifications
- References
| Catalog No. | PA007755.h1Fs-b |
|---|---|
| Product Name | Biotinylated Recombinant Anti-human CD34 Monoclonal Antibody (Clone: 581), Human IgG1 Fc Silent Kappa |
| Supplier Name | Syd Labs, Inc. |
| Brand Name | Syd Labs |
| Clone | 581 |
| Isotype | Human IgG1 Fc Silent Kappa. |
| Source/Host | The site-directed biotinylated anti-human CD34 monoclonal antibody (clone: 581) was produced in mammalian cells. |
| Specificity/Sensitivity | The site-directed biotinylated recombinant 581 antibody specifically binds to human CD34. |
| Applications | Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads. |
| Form Of Antibody | 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives. |
| Conjugate | Biotin-conjugated. |
| Purity | >95% by SDS-PAGE under reducing conditions. |
| Shipping | The 581 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied. |
| Note | Recombinant mouse IgG1 isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator. |
| Order Offline | Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card. |
Description
PA007755.h1Fs-b: Biotinylated Recombinant Anti-human CD34 Monoclonal Antibody (Clone: 581), Human IgG1 Fc Silent Kappa
Recombinant human IgG1 monoclonal antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
References of Anti-Human CD34 Monoclonal Antibody (Clone: 581):
1. Deep Immunophenotyping of Human Whole Blood by Standardized Multi-parametric Flow Cytometry Analyses
Kvistborg, P., et al. Front Immunol. 2023 May 18;14:1163618. PMID: 37265694
“The anti-human CD34 clone 581 (BD Biosciences) was utilized in an 11-color antibody cocktail for flow cytometric identification of hematopoietic stem cells in whole blood samples. The panel enabled simultaneous detection of CD34+ progenitors and mature leukocytes, with data acquired on a BD FACSCanto II and analyzed using FlowJo v10. Standardization ensured reproducible CD34 expression patterns across laboratories in healthy donors.”
2. Standardizing Flow Cytometry Immunophenotyping Analysis from the Human ImmunoPhenotyping Consortium
Maecker, H. T., et al. Sci Rep. 2016 Feb 11;6:20686. PMID: 26859299
“The 8-color flow cytometry panel incorporated anti-human CD34 clone 581 for enumerating stem cell populations in thawed PBMCs. Data from 30 healthy donors validated inter-lab consistency in CD34+ cell gating, with viability dye and isotype controls minimizing non-specific binding. Clone 581 demonstrated high sensitivity for detecting rare progenitors in cryopreserved samples.”
3. Flow cytometric enumeration of CD34+ hematopoietic stem and progenitor cells. European Working Group on Clinical Cell Analysis
Sutherland, D. R., et al. J Hematother. 1996 Summer;5(3):213-22. PMID: 9271794
“The anti-human CD34 monoclonal antibody clone 581 (Becton Dickinson) was selected for its specificity in flow cytometric enumeration, using a single-platform method with Trucount beads for absolute CD34+ cell counting in mobilized blood. Gating excluded doublets and debris, with validation against colony-forming assays and low inter-laboratory variability confirming clone 581’s reliability.”
4. Sensitive detection and enumeration of CD34+ cells in peripheral and cord blood by flow cytometry
Bender, J. G., et al. Clin Cancer Res. 1996 Aug;2(8):1527-33. PMID: 8682641
“Flow cytometry with PE-conjugated anti-human CD34 clone 581 detected as few as 0.01% CD34+ cells in lysed peripheral and cord blood samples with high precision. Comparison with immunofluorescence microscopy validated the approach, and CD34+ progenitor frequencies varied significantly between donors, highlighting the assay’s sensitivity.”
5. Comparative evaluation of commonly used clones and fluorochrome conjugates of monoclonal antibodies for CD34 antigen detection
Schuurman, H. J., et al. Cytometry. 1999 Nov 15;38(1):39-47. PMID: 10533724
“The anti-human CD34 clone 581 exhibited superior performance in direct immunofluorescence flow cytometry, with PE-conjugated clone 581 providing the highest signal-to-noise ratio for rare cell detection on KG-1a cells. Minimal cross-reactivity with non-hematopoietic cells and its favorability in multi-color panels supported its clinical use recommendations.”
For more technical references or data sheets regarding the Recombinant Anti-Human CD34 Monoclonal Antibody (Clone: 581) formats, please contact our scientific support team at message@sydlabs.com or explore options via Syd Labs.
Syd Labs provides the following recombinant anti-human CD34 monoclonal antibodies for flow cytometry:
Recombinant anti-human CD34 monoclonal antibodies (clone QBEnd/10) for flow cytometry
Recombinant anti-human CD34 monoclonal antibodies (clone My10) for flow cytometry
Recombinant anti-human CD34 monoclonal antibodies (clone 581) for flow cytometry
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