Catalog No.	PA008000.m2cLA
Product Name	Anti-mouse PD-1 / PD-1 Bispecific Antibody (RMP1-14.1 / 29F.1A12.1) | PA008000.m2cLA
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	Programmed Cell Death Protein 1, PD-1, CD279, Cluster of Differentiation 279, RMP1-14
Clone	RMP1-14.1, the same variable region sequences as the rat anti-mouse PD-1 monoclonal antibody (clone number: RMP1-14). 29F.1A12.1, the same variable region sequences as the rat anti-mouse PD-1 monoclonal antibody (clone number: 29F.1A12).
Isotype	mouse IgG2c, kappa.
Applications	immunohistochemistry (IHC), Flow Cytometry (FC), and various in vitro and in vivo functional assays.
Immunogen	The original rat hybridoma (clone name: RMP1-14) was generated by immunizing rats with mouse PD-1-transfected BHK cells. The original rat hybridoma (clone name: 29F.1A12) was generated by immunizing rats with the mouse PD-1 cDNA followed by the mouse PD-1-Ig fusion protein.
Form Of Antibody	0.2 μM filtered solution of 1x PBS.
Endotoxin	Less than 1 EU/mg of protein as determined by LAL method.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The In Vivo Grade Recombinant Anti-mouse PD-1 / PD-1 Bispecific Antibody, Mouse IgG2c LALAPG Kappa (Clone RMP1-14.1 / 29F.1A12.1) are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 3 month from date of receipt, 2 to 8°C as supplied. 12 months from date of receipt, -20°C to -70°C as supplied.
Note	Recombinant anti-mouse PD 1 / PD 1 bispecific antibodies share the variable region sequences with the rat anti-mouse PD-1 antibody (clone number: RMP1-14) and the rat anti-mouse PD-1 antibody (clone number: 29F.1A12). The recombinant mouse IgG2c LALAPG bispecific isotype control antibody available.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA008000.m2cLA: In Vivo Grade Recombinant Anti-mouse PD-1 / PD-1 Bispecific Antibody, Mouse IgG2c LALAPG Kappa (Clone RMP1-14.1 / 29F.1A12.1)

Background
Are you looking for a better anti-mouse PD-1 monoclonal antibody than the clones of RMP1-14 and 29F.1A12? The rat anti-mouse PD-1 monoclonal antibodies (clone No. RMP1-14, rat IgG2a kappa, and clone No. 29F.1A12, rat IgG2a kappa) bind to different epitopes of the mouse PD-1 protein (CD279 or programmed death-1) encoded by the mouse pdcd1 gene, a member of the CD28 family of the Ig superfamily. PD-1 has two ligands, PD-L1 and PD-L2, both of which belong to the B7 family. It has been shown that in mouse models of melanoma, tumor growth can be transiently arrested via treatment with the anti-mouse PD 1 antibody and the anti-mouse PD L1 antibody which block the interaction between the PD-L1 protein and its receptor PD-1 protein. Both RMP1-14 and 29F.1A12 antibodies block the binding of both the mouse PD-L1 protein and the mouse PD-L2 protein to the mouse PD-1 protein. It is under investigation whether the affinity of the recombinant anti-mouse PD-1 / PD-1 bispecific antibodies (RMP1-14 / 29F.1A12 and 29F.1A12 / RMP1-14) is higher than the individual monoclonal antibodies and their combination.

Our recombinant anti-mouse PD-1 / PD-1 bispecific antibodies (combination of RMP1-14 and 29F.1A12) have a part (variable regions) or complete amino acid sequences of the rat anti-mouse PD-1 antibody (hybridoma clone name or number: RMP1-14 and 29F.1A12).

Recombinant anti-HEL / HEL Bispecific Isotype Control Antibodies:
Recombinant anti-HEL / HEL Mouse IgG1 Kappa Bispecific Isotype Control Antibody, In vivo grade
Recombinant anti-HEL / HEL Mouse IgG2a Kappa Bispecific Isotype Control Antibody, In vivo grade
Recombinant anti-HEL / HEL Mouse IgG2c Kappa Bispecific Isotype Control Antibody, In vivo grade

Syd Labs provides the following anti-mouse PD-L1 / PD-1 monoclonal antibodies:
Recombinant anti-mouse PD1 antibodies (Clone 29F.1A12.1), In vivo grade
Recombinant anti-mouse PD-1 antibodies (Clone RMP1-14.1), In vivo grade
Recombinant anti-mouse PD-L1 antibodies (Clone 10F.9G2.1), In vivo grade

Syd Labs provides the following anti-mouse PD-L1 and PD-1 bispecific antibodies:
Recombinant anti-mouse PD-1 / PD-1 bispecific antibodies (Clone RMP1-14.1 / 29F.1A12.1), In vivo grade
Recombinant anti-mouse PD-1 / PD-1 bispecific antibodies (Clone 29F.1A12.1 / RMP1-14.1), In vivo grade
Recombinant anti-mouse PD-1 / PD-L1 bispecific antibodies (Clone RMP1-14.1 / 10F.9G2.1), In vivo grade
Recombinant anti-mouse PD-L1 / PD-1 bispecific antibodies (Clone 10F.9G2.1 / RMP1-14.1), In vivo grade
Recombinant anti-mouse PD-1 / PD-L1 bispecific antibodies (Clone 29F.1A12.1 / 10F.9G2.1), In vivo grade
Recombinant anti-mouse PD-L1 / PD-1 bispecific antibodies (Clone 10F.9G2.1 / 29F.1A12.1), In vivo grade

Questions and Answers about recombinant anti-mouse PD-1 / PD-1 bispecific antibody (Clone RMP1-14 / 29F.1A12):
Question:How did you design the anti-mouse PD1 / PD1 bispecific antibody?
Answer:Clones RMP1-14 and 29F.1A12 are the most well-known anti-mouse PD1 antibodies available in the market. They are commonly used to block PD-1 signaling in vivo in murine tumor models and other mouse models. These two clones both have extensive multi-year publication records supporting them. The two antibodies bind to different epitopes of the mouse PD1 protein. In addition, the mouse IgG2c antibody is produced in certain inbred strains such as C57BL/6, C57BL/10, SJL, and NOD, which does not express the mouse IgG2a antibody; the mouse IgG2a antibody is produced in other inbred strains such as BALB/c and Swiss Webster mice, which does not express the mouse IgG2c antibody. Because the C57BL/6 mouse strain is used in most of animal tumor model research, it is better to use the IgG2c antibodies rather than the IgG2a antibodies. The format with the Fc silenced, Fc silent, or Fcs with silenced effector function, such as LALAPG mutation, is the most popular for anti-mouse PD1 antibodies (clones RMP1-14 and 29F.1A12) and anti-mouse PD-L1 antibodies (clone 10F.9G2).
Question:Do you produce any recombinant Fc-silenced RMP1-14 / 29F.1A12 or 29F.1A12 / RMP1-14 bispecific antibody?
Answer:Sure, we provide various recombinant Fc silent RMP1-14 / 29F.1A12 and 29F.1A12 / RMP1-14 bispecific antibodies, such as mIgG2c LALAPG, mIgG2a LALAPG, and mIgG1 D265A. We also provide custom recombinant antibody production service to produce other engineered versions of recombinant RMP1-14 / 29F.1A12 and 29F.1A12 / RMP1-14 bispecific antibodies.

Anti-mouse PD-1 / PD-1 Antibody (RMP1-14.1 / 29F.1A12.1) from: In Vivo Grade Recombinant Anti-mouse PD-1 / PD-1 Bispecific Antibody, Mouse IgG2c LALAPG Kappa (Clone RMP1-14.1 / 29F.1A12.1): PA008000.m2cLA Syd Labs

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