Catalog No.	PA007453
Product Name	Anti-Human HLA-DR Antibody, clone L243 | PA007453
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	DRA/DRB1, HLA class II histocompatibility antigen DR alpha/ DRB1-15 beta chain, HLA-DRA1/DRB1, MHC class II antigen DRA
Clone	L243.
Isotype	Mouse IgG2a Kappa.
Specificity/Sensitivity	The in vivo grade recombinant mouse monoclonal antibody (clone: L243) specifically binds to mouse HLA-DR.
Applications	ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse HLA-DR protein.
Form Of Antibody	0.2 μM filtered solution of 1x PBS.
Endotoxin	Less than 1 EU/mg of protein as determined by LAL method.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The in vivo grade recombinant anti-human HLA-DR antibodies (clone of L243) are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant Mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007453: Recombinant Anti-Human HLA-DR Monoclonal Antibody (Clone: L243), Mouse IgG2a Kappa, In vivo Grade

The in vivo grade recombinant anti-human HLA-DR Mouse IgG2a Kappa monoclonal antibody was produced in mammalian cells. The anti-human HLA-DR antibody is a specialized reagent that specifically targets the HLA-DR protein, a key component of the human Major Histocompatibility Complex (MHC) Class II molecules. HLA-DR is primarily expressed on professional antigen-presenting cells (APCs) such as B cells, monocytes, macrophages, dendritic cells, and activated T cells.

In immunology research and diagnostics, anti-human HLA-DR antibodies serve as essential tools for identifying and analyzing MHC Class II-expressing immune cells. They are widely used in techniques including:

• Flow Cytometry (FC) for quantifying HLA-DR expression on cell surfaces.
• Immunohistochemistry (IHC) to visualize HLA-DR-positive cells in tissue sections.
• Immunofluorescence (IF) for detailed cell identification and immune response studies.

Common monoclonal clones include L243 (highly specific for HLA-DR), LN3, and YD1/63.4.10. These antibodies are often available conjugated to fluorophores such as FITC, PE, or APC for multi-color experiments.

The term anti-HLA-DR antibody also refers to naturally occurring alloantibodies produced in individuals following exposure to foreign HLA antigens, such as during pregnancy, blood transfusions, or organ transplantation. These patient-derived antibodies can react with donor HLA-DR molecules, increasing the risk of graft rejection in transplant recipients.

In clinical practice, detecting pre-existing anti-HLA-DR antibodies is critical for HLA typing and compatibility testing prior to transplantation, helping to minimize rejection risks. While these antibodies are generally harmless to the individual producing them, they play a significant role in transplant immunology.

Whether used as a research reagent or identified in clinical settings, the anti-human HLA-DR antibody is indispensable for studying immune cell function, analyzing antigen presentation, and improving outcomes in organ transplantation.

References of Anti-Human HLA-DR Monoclonal Antibody:

1、Citrullinated Vimentin Presented on MHC-II in Tumor Cells Is a Target for CD4+ T-Cell-Mediated Antitumor Immunity.
Brentville, V. A., et al. Cancer Res. 2016 Feb 1;76(3):548-60. doi: 10.1158/0008-5472.CAN-15-1085. PMID: 26719533.
“To construct the plasmid pVitro 2 Chimeric HLA-DR401, cDNA was generated from mRNA isolated from the splenocytes of transgenic HLA-DR4 mice. …Following sequence confirmation full-length chimeric α chain comprising murine H2-Ea with the human HLA-DRA α 1 domain was ligated into the FspI/EcoRI mcs2 of the vector pVITRO2-hygro-mcs (Invivogen). …The CMV promoter within each cassette was excised and replaced with the IFNγ-inducible promoter driving expression of the HLA-DR401 chains within the pDCOrig vector backbone. …B16F1 melanoma, LLC/2 lung carcinoma, and Pan02 pancreatic cancer cells were transfected using the Lipofectamine Transfection Reagent (Invitrogen) with 4 μg of the plasmid pVitro 2 Chimeric HLA-DR401 that encodes both full-length chimeric α and β chains according to the manufacturer’s instructions. …Lines were cloned by limiting dilution and expression was confirmed by flow cytometry using the HLA-DR PE conjugated antibody (clone L243) from eBioscience or the murine MOG antibody (clone 8-18C5, Millipore) with the anti-mouse IgG1 AlexaFluor 647 secondary antibody (Life Technologies).”

2、The Possible Mechanism of Idiosyncratic Lapatinib-Induced Liver Injury in Patients Carrying Human Leukocyte Antigen-DRB1*07:01.
Hirasawa, M., et al. PLoS One. 2015 Jun 22;10(6):e0130928. doi: 10.1371/journal.pone.0130928. PMID: 26098642.
“HLA-peptide complexes were then captured in triplicate on a 96-well EIA/RIA plate (Corning, #3361) coated with L243 anti-HLA-DRA antibody (BioXCell, #BE0160). …The finding motivated us to predict the possible interaction mode of lapatinib with HLA-DRs. …Considering the enhancing effect of abacavir on the binding of LF9 peptide to HLA-B*57:01, the destabilizations of several pockets by a small molecular drug do not necessarily lead to the displacement of the ligand peptide. …This notable narrow binding groove was observed only in the lapatinib-DRB1*07:01 system. …In summary, our in vitro experiments revealed that lapatinib enhances binding of the ligand peptide to HLA-DRB1*07:01 preferentially.”

3、Preserved dendritic cell HLA-DR expression and reduced regulatory T cell activation in asymptomatic Plasmodium falciparum and P. vivax infection.
Kho, S., et al. Infect Immun. 2015 Aug;83(8):3224-32. doi: 10.1128/IAI.00226-15. PMID: 26034211.
“The activation/maturation of circulating blood DC was examined by measuring the median fluorescence intensity of HLA-DR expression. …In contrast, during acute uncomplicated P. falciparum or P. vivax malaria, DC HLA-DR expression was significantly lower (P = 0.0001 and P < 0.0001, respectively). …In both mDC subsets, HLA-DR was preserved during asymptomatic infection, but in 3 adults with uncomplicated malaria, HLA-DR expression appeared reduced. …Our DC HLA-DR data support the earlier description of HLA-DR retention by circulating blood DC in Fulani children, which is understood to contribute to protective immunity against malaria. …Studies on Treg cell activation in asymptomatic Plasmodium infection are limited.”

4、Zwitterionic polysaccharides stimulate T cells by MHC class II-dependent interactions.
Tzianabos, A. O., et al. J Immunol. 2002 Dec 1;169(11):6149-53. doi: 10.4049/jimmunol.169.11.6149. PMID: 12444118.
“The authors’ informatics analyses should allow them to draw concrete conclusions, supported by the data, about the biology of the system(s) under study. …However, it is not necessary for either type of manuscript to include definitive mechanistic analysis or experiments beyond those necessary for the acquisition of reproducible and meaningful data sets. …The latter type of manuscript must convincingly demonstrate the utility of the new analysis method to reveal novel biological insights about the system(s) under study. …Cutting Edge is the rapid publication section of The JI, presenting reports describing significant advances. …Immunology Notes And Resources is a feature where items of general interest to the immunology community such as articles on nomenclature or other significant items that may impact scientific research.”

5、Differential incorporation of CD45, CD80 (B7-1), CD86 (B7-2), and major histocompatibility complex class I and II molecules into human immunodeficiency virus type 1 virions and microvesicles: implications for viral pathogenesis and immune regulation.
Esser, M. T., et al. J Virol. 2001 Jul;75(13):6173-82. doi: 10.1128/JVI.75.13.6173-6182.2001. PMID: 11390619.
“Immunofluorescent staining of PBMC and MDMs (3 × 105 per condition) was performed at 4°C for 30 min by using isotype immunoglobulin G1 (IgG1) (X40), IgG2a (X39), and V4 (non-gp120-interacting domain on CD4) and HLA-DR (L243) MAbs from Becton Dickinson Immunocytometry Systems (San Jose, Calif.). …Future studies will determine whether the HLA-DR phenotype of the virus or the responder PBMC affects HIV-1-triggered apoptosis. …We thank Mike Grimes and Bill Bohn for the production and characterization of sucrose-banded, purified HIV-1NL4-3, Antonio Valentin and Jim Turpin for the generous gifts of selected HIV-1 isolates, and Darlene Marti and Mary Carrington for HLA-DR genotyping of the PBMC donor and cell lines. …Human immunodeficiency virus (HIV) infection results in a functional impairment of CD4+ T cells long before a quantitative decline in circulating CD4+ T cells is evident. …Because T-cell receptor engagement of major histocompatibility complex (MHC) molecules in the absence of costimulation mediated via CD28 binding to CD80 (B7-1) or CD86 (B7-2) can lead to anergy or apoptosis, we determined whether HIV type 1 (HIV-1) virions incorporated MHC class I (MHC-I), MHC-II, CD80, or CD86. Microvesicles produced from matched uninfected cells were also evaluated.”

6、Lipopolysaccharide stimulates the proliferation of human CD56+CD3- NK cells: a regulatory role of monocytes and IL-10.
Goodier, M. R., et al. J Immunol. 2000 Jul 1;165(1):139-47. doi: 10.4049/jimmunol.165.1.139. PMID: 10861046.
“NK cells recognize and kill tumor cells and normal cells, and these play an important role in immune defense in cancer, infectious disease, and autoimmunity. …However, the mechanisms controlling the proliferation and maintenance of NK cells in normal human individuals are less clearly defined. …This enhancement is also reflected in substantial increases in cytolytic activity and IFN-γ production. …On the other hand, mRNA for the p35 and p40 subunits of IL-12 is still induced in CD14-depleted cultures. …Expansion of CD3−CD56+ cells was reconstituted in MHC class II-depleted cell cultures by adding back monocyte-derived dendritic cells.”

For more references about Anti-Human HLA-DR Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.

Related Recombinant IgG Reference Antibodies:
Recombinant mouse IgG2a isotype control antibody, In vivo grade

Anti-Human HLA-DR (L243) from: Anti-Human HLA-DR Monoclonal Antibody, Mouse IgG2a Kappa (Clone: L243): PA007453 Syd Labs

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