Catalog No.	PA008008.m1
Product Name	Anti-human CD3 / CD3 Bispecific Antibody (SP34-2 / OKT3) | PA008008.m1
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	cluster of differentiation 3, CD3D, CD3E, CD3G
Clone	SP34-2 / OKT3.
Isotype	mouse IgG1, lambda.
Applications	immunohistochemistry (IHC), Flow Cytometry (FC), and various in vitro and in vivo functional assays.
Form Of Antibody	0.2 μM filtered solution of 1x PBS.
Endotoxin	Less than 1 EU/mg of protein as determined by LAL method.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The in vivo grade recombinant mouse anti-human CD3 / CD3 bispecific antibodies are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 3 month from date of receipt, 2 to 8°C as supplied. 12 months from date of receipt, -20°C to -70°C as supplied.
Note	Recombinant anti-human CD3 / CD3 bispecific antibodies have the same variable region sequences as the mouse anti-human CD3 antibody (clone number: OKT3) and the mouse anti-human CD3 antibody (clone number: SP34-2). The recombinant mouse IgG2a bispecific isotype control antibody available.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA008008.m1: Recombinant Anti-human CD3 / CD3 Bispecific Antibody, Mouse IgG1 lambda (Clone SP34-2 / OKT3), In Vivo Grade

The in vivo grade recombinant mouse anti-human CD3 / CD3 bispecific antibody (mouse IgG1 lambda) was produced in mammalian cells.

The OKT3 antibody binds to CD3 epsilon of the T cell receptor-CD3 complex found only on mature T cells and medullary thymocytes. An interaction between T cells, OKT3, and monocytes causes T-cell activation (mitogenesis) in vitro. On the other hand, the OKT3 antibody blocks the unique association between the antigen receptor and CD3, and both the generation and function of cytotoxic T cells. After an initial dose of the OKT3 antibody, T cells disappear from the circulation within minutes to hours. During treatment with the OKT3 antibody, T cells bearing the usual array of surface molecules (CD2, CD4, and CD8) reappear in the peripheral blood circulation but these cells are devoid of CD3. After another 48 hours without the OKT3 antibody, the normal array of surface molecules including CD3 appear again on all T cells. The selective removal of CD3 by internalization is thought to be the key mechanism of action of the OKT3 antibody. Comodulation of the antigen receptor with CD3 explains the immunoblocking action of the OKT3 antibody in vivo. The OKT3 antibody is ineffective clinically if the CD3 target molecule is not modulated. The OKT3 antibody is effective for induction of immunosuppression and for treating initial and steroid-resistant rejections.

The anti-human CD3 antibody (clone: SP34-2), a derivative of SP34, is known to cross-react with a major subset of peripheral blood lymphocytes in both human and non-human primates, including baboon, rhesus, cynomolgus, and pigtail macaque monkeys. The SP34-2 antibody specifically recognizes a major subset of CD3-positive cells from non-human primates, similar to its human counterpart, but does not react with monocytes or granulocytes. The SP34-2 antibody is used in flow cytometry to identify and analyze CD3-positive cells in human and various non-human primate species. It can also be used to stimulate cell proliferation in various primate peripheral blood mononuclear cells (PBMC).

Our recombinant anti-human OKT3 / SP34-2 bispecific antibodies have a part (variable regions) or complete amino acid sequences of the mouse anti-human CD3 antibody (hybridoma clone name or number: OKT3 and SP34-2). It is under investigation whether either of the recombinant anti-human CD3 / CD3 bispecific antibodies (OKT3 / SP34-2 and SP34-2 / OKT3) is the best anti-human CD3 antibody.

Recombinant anti-HEL / HEL Bispecific Isotype Control Antibodies:

In vivo Grade Recombinant anti-HEL / HEL Mouse IgG1 Kappa Bispecific Isotype Control Antibody
In vivo Grade Recombinant anti-HEL / HEL Mouse IgG2a Kappa Bispecific Isotype Control Antibody
In vivo Grade Recombinant anti-HEL / HEL Mouse IgG2c Kappa Bispecific Isotype Control Antibody

Syd Labs provides the following in vivo grade recombinant anti-human CD3 monoclonal antibodies:

Muromonab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Teplizumab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Foralumab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Recombinant Anti-human CD3 monoclonal antibody (Clone: OKT3)
Recombinant Anti-human CD3 monoclonal antibody (Clone: SP34-2)
Recombinant Anti-human CD3 monoclonal antibody (Clone: UCHT1)
Recombinant Anti-human CD3 monoclonal antibody (Clone: 12F6)
Recombinant Anti-human CD3 / CD3 Bispecific Antibody (Clone: OKT3 / UCHT1)
Recombinant Anti-human CD3 / CD3 Bispecific Antibody (Clone: OKT3 / SP34-2)
Recombinant Anti-human CD3 / CD3 Bispecific Antibody (Clone: SP34-2 / OKT3)

Syd Labs provides the following in vivo grade recombinant anti-mouse CD3 monoclonal antibodies:

Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 145-2C11)
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 500A2)
Recombinant Anti-mouse CD3 monoclonal antibody (Clone: 17A2)

Syd Labs provides the following recombinant anti-mouse CD3 monoclonal antibodies for flow cytometry:

Recombinant Anti-mouse CD3 monoclonal antibody (Clone: 17A2) for flow cytometry
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 145-2C11) for flow cytometry
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 500A2) for flow cytometry

Syd Labs provides the following recombinant anti-human CD3 monoclonal antibodies for flow cytometry:

Recombinant Anti-human CD3 monoclonal antibody (Clone: OKT3) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: UCHT1) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: 12F6) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: SP34-2) for flow cytometry

Questions and Answers about recombinant anti-human CD3 / CD3 bispecific antibody (Clone OKT3 / SP34-2):

Question: How did you design the anti-human CD3 / CD3 bispecific antibody?
Answer: Clones OKT3 and SP34-2 are well-known anti-human CD3 antibodies available in the market. These two clones both have extensive multi-year publication records supporting them. The two antibodies bind to different epitopes of the human CD3 protein. Thus, the OKT3 / SP34-2 bispecific antibody may behave different from the individual or combined OKT3 and SP34-2 antibodies.

Question: Do you produce any recombinant Fc-silenced OKT3 / SP34-2 or SP34-2 / OKT3 bispecific antibody?
Answer: We provide custom recombinant antibody production service to produce engineered versions of recombinant OKT3 / SP34-2 and SP34-2 / OKT3 bispecific antibodies, such as mIgG2c LALAPG, mIgG2a LALAPG, and mIgG1 D265A.

Anti-human CD3 / CD3 Bispecific Antibody (SP34-2 / OKT3) from: In Vivo Grade Recombinant Anti-human CD3 / CD3 Bispecific Antibody, Mouse IgG2a Kappa (Clone SP34-2 / OKT3): Syd Labs PA008008.m1

 

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