UNveil Unstained Protein Ladder（10 to 200 kDa）
$88.00
BIO-HELIX UNveil Unstained Protein Ladder（10 to 200 kDa） (catalog No. PMU12-0500), The Unveil Unstained Protein Ladder (12 unstained recombinant proteins, 10 to 200 kDa). The intensified 25kDa and 85kDa protein bands serving as the two reference bands.
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| Catalog No. | PMU12-0500 |
|---|---|
| Product Name | UNveil Unstained Protein Ladder（10 to 200 kDa） |
| Supplier Name | BIO-HELIX |
| Brand Name | BIO-HELIX |
| Order Offline | Syd Labs, Inc. 4 Avenue E, Hopkinton, MA 01748 USA. Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com |
Description
PMU12-0500: UNveil Unstained Protein Ladder（10 to 200 kDa）
The Unveil Unstained Protein Ladder is a mixture of 12 unstained recombinant proteins covering a wide range of molecular weights from 10 to 200 kDa. The proteins resolve into clearly identifiable bands when separated on the SDS-PAGE gel (MES buffer), with the intensified 25kDa and 85kDa protein bands serving as the two reference bands. The Unveil Unstained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in the gel loading buffer and is ready to use.
3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.
Storage Buffer :
20 mM Tris-phosphate(pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol.
Quality Control :
Under suggested conditions, Unveil Unstained Protein Ladder resolves 12 major bands in 4-12%Bis- Tris Gel(MES buffer) and after Western blotting to the nitrocellulose membrane.
Storage :
Stable for up to 2 weeks at 25°C.
Stable for up to 3 months at 4°C.
Stable for up to 24 months at -20°C.
Note. The apparent molecular weight (kDa) of each protein has been determined by calibration against unstained protein standards; supplemental data should be considered for more accurate adjustment in different electrophoresis conditions.
All products are for research use only.
Caution :
Not intended for human or animal diagnostic or therapeutic uses.
Required materials but not provided :
Vertical Electrophoresis system
Power supplies
Vortex or equivalent
Microcentrifuge
Please remember our product information: UNveil Unstained Protein Ladder（10 to 200 kDa） catalog number: PMU12-0500 BIO-HELIX