Has anyone used your electroporation machine for antibody transient production in CHO cells? Almost all well-known CRO companies in China have purchased and used the electroporation machine SLT for recombinant antibody transient production in CHO...
Can the electroporation model SLT be used for stable transfection in addition to transient transfection? Transient transfection and stable transfection the plasmid vectors used make the major difference.
What is the problem with transient transfection to CHO cells? Both chemical transfection and electroporation-based transfection methods have been tried to improve the efficiency and yield of protein transient production in CHO cells.
Celetrix PBMC cell electroporation upgrade model EX+ (11-0106) is an upgraded version of model LE+ (11-0101) with larger electroporation volume (600 ul and 1 ml electroporation tubes) and the option of the electrofusion function for...
Celetrix T cell electroporation upgrade model LE+ (11-0101) can be used to transfect a variety of mammalian cell line, especially PBMC, with high-efficiency and low-toxicity electroporation. It can maintain a high level of T cell...
Recombinant Anti-Myc-Tag Mouse IgG1 Monoclonal Antibody (Clone 9E10). Recombinant Myc-Tag monoclonal antibodies (clone 9E10) with various isotypes; low prices for bulk order.
Extensive experience with making DNA constructs for Fc fusion protein production, expressing and purifying a number of Fc fusion proteins from microgram to kilogram scales in mammalian cells, such as CHO and HEK 293 cells.
Recombinant antibody (IgG) production services include: molecular construction of recombinant IgG antibodies; transient production of recombinant antibodies; purification of recombinant antibodies. Antibody isotype switching or class switching is available.
Recombinant antibody (scFv-Fc) production services include: molecular construction of recombinant scFv-Fc antibodies; transient production of recombinant antibodies; purification of recombinant antibodies.
We can optimize stable and transient expression for secreted or intracellular, cytosolic or periplasmic, tagged or untagged proteins. We assess expression levels using the methods of SDS-PAGE, Western, ELISA or functional analysis.
