IRIS9 Plus Prestained Protein Ladder
$80.00
BIO-HELIX IRIS9 Plus Prestained Protein Ladder (catalog No. PMI09-0500), The IRIS9 Plus Prestained Protein Ladder (9 pre-stained proteins, 15 to 170 kDa). A green band at 15 kDa, a red band at 70 kDa, and a peacock green band at 60 kDa.
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| Catalog No. | PMI09-0500 |
|---|---|
| Product Name | IRIS9 Plus Prestained Protein Ladder |
| Supplier Name | BIO-HELIX |
| Brand Name | BIO-HELIX |
| Order Offline | Syd Labs, Inc. 4 Avenue E, Hopkinton, MA 01748 USA. Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com |
Description
PMI09-0500: IRIS9 Plus Prestained Protein Ladder
The IRIS9 Plus Prestained Protein Ladder is a combination of 9 pre-stained proteins with molecular weights from 15 to 170 kDa. The 9 recombinant proteins are covalently coupled with blue chromophore, while 1 green band at 15 kDa, a red band at 70 kDa and a newly designed peacock green band at 60 kDa serve as reference bands. The IRIS9 Plus Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating the target protein size and validating the Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.
3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.
Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.
Storage Buffer :
0.2~0.4 mg/ml of each band of protein is stored in a mixture of solution that contains: 20 mM Trisphosphate at pH 7.5, 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol.
Quality Control :
5μl of IRIS9 Plus Prestained Protein Ladder (PMI09) resolves 9 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane.
Storage :
Stable for up to 2 weeks at 25°C.
Stable for up to 3 months at 4°C.
Stable for up to 24 months at -20°C.
Required materials but not provided：
Vertical Electrophoresis system
Power supplies
Vortex or equivalent
Microcentrifuge
Please remember our product information: IRIS9 Plus Prestained Protein Ladder catalog number: PMI09-0500 BIO-HELIX