Biotinylated Anti-mouse CTLA-4 Antibody (9D9, Human IgG1 Fc Silent Kappa)
$90.00 – $180.00
Recombinant mouse anti-mouse CTLA-4 / CD152 monoclonal antibody (Clone 9D9, Biotinylated), engineered with a Human IgG1 Fc-Silent domain to minimize non-specific Fc receptor binding during flow cytometry and immune checkpoint analysis. Variable regions are derived from the classic rat anti-mouse CTLA-4 clone 9D9 and recombinantly expressed in mammalian cells. For human T cell research, Syd Labs OKT3 antibodies are also available.
- Form & Conjugation: Biotinylated, Flow Cytometry Grade
- Applications: Flow Cytometry (FC), Immune Checkpoint Analysis, Functional Assays
- Alternative Formats: Non-biotinylated FC grade, unconjugated in vivo grade, and Syd Labs OKT3 antibodies are also available.
- Details & Specifications
- References
| Catalog No. | PA007730.h1Fs-b |
|---|---|
| Product Name | Biotinylated Recombinant Anti-mouse CTLA-4 Monoclonal Antibody (Clone: 9D9), Human IgG1 Fc Silent Kappa |
| Supplier Name | Syd Labs, Inc. |
| Brand Name | Syd Labs |
| Clone | 9D9. |
| Isotype | Human IgG1 Fc Silent Kappa |
| Source/Host | The site-directed biotinylated anti-mouse CTLA-4 monoclonal antibody (clone: 9D9) was produced in mammalian cells. |
| Specificity/Sensitivity | The site-directed biotinylated recombinant 9D9 antibody specifically binds to mouse CTLA-4 protein |
| Applications | Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads |
| Form Of Antibody | 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives. |
| Conjugate | Biotin-conjugated |
| Purity | >95% by SDS-PAGE under reducing conditions and HPLC |
| Shipping | The 9D9 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied. |
| Note | Recombinant rat IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator. Recombinant anti-mouse GITR monoclonal antibodies from the variable region sequences of the rat anti-mouse GITR monoclonal antibody (clone number: DTA-1) are produced from mammalian cells and good for in vitro and in vivo studies. |
| Order Offline | Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card. |
Description
PA007730.h1Fs-b: Biotinylated Recombinant Anti-mouse CTLA-4 Monoclonal Antibody (Clone: 9D9), Human IgG1 Fc Silent Kappa
Recombinant human IgG1 monoclonal antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
The 9D9 antibody blocks the mouse cytotoxic T lymphocyte antigen-4 (CTLA-4). Blocking CTLA-4 removes an inhibitory signal from reducing the activity of T lymphocytes.
Cytotoxic T-lymphocyte antigen-4 (CTLA-4) is an inhibitory molecule that competes with the stimulatory CD28 for binding to B7 on antigen presenting cells. CTLA-4 and CD28 are both presented on the surface of T-cells.
References of Anti-Mouse CTLA-4 Monoclonal Antibody (Clone: 9D9):
Simpson, T. R., et al. J Exp Med. 2013 Aug 26;210(9):1695-710. PMID: 23878181
“The study investigates the mechanism of anti-CTLA-4 therapy using the 9D9 monoclonal antibody in a B16 melanoma mouse model. …Tumor-infiltrating lymphocytes were isolated from B16 tumors and stained with fluorescently conjugated antibodies against CD4, CD25, CTLA-4 (clone 9D9), FoxP3, CD103, CD8, and CD45.1. …Anti-CTLA-4 therapy significantly increased CD8+ T cell infiltration in tumors, highlighting the Fc-dependent depletion of regulatory T cells as a key factor in therapeutic efficacy.”
2. Anti-CTLA-4 therapy broadens the melanoma-reactive CD4+ T cell response
Quezada, S. A., et al. Oncoimmunology. 2014;3(5):e29032. PMID: 24828551
“This study explores the effects of anti-CTLA-4 therapy using the 9D9 monoclonal antibody on melanoma-reactive CD4+ T cells. …Splenocytes were harvested and stained with fluorescently conjugated antibodies against CD4, CD8, CD25, CD44, CTLA-4 (clone 9D9), and PD-1 for flow cytometry analysis. …Tetramer staining identified melanoma-specific CD4+ T cells, revealing a 3-fold increase in their frequency following anti-CTLA-4 treatment.”
3. CTLA-4 expression on activated T cells by flow cytometry with clone 9D9
Egen, J. G., et al. Immunity. 2006 Jun;24(6):803-14. PMID: 16751384
“This study examines CTLA-4 expression on activated T cells using the 9D9 monoclonal antibody. …Activated T cells were stained with PE-conjugated anti-mouse CTLA-4 (clone 9D9), revealing rapid upregulation of CTLA-4 within 24 hours of activation via flow cytometry. …Blockade with anti-CTLA-4 enhanced T cell expansion, demonstrating the role of CTLA-4 in regulating T cell responses in animal models.”
For more technical references or data sheets regarding the Anti-Mouse CTLA-4 Monoclonal Antibody (Clone: 9D9) formats, please contact our scientific support team at message@sydlabs.com.
Syd Labs provides the following in vivo grade recombinant anti-mouse CTLA-4 monoclonal antibodies:
Recombinant Anti-mouse CTLA-4 monoclonal antibody (Clone: 9D9)
Recombinant Anti-mouse CTLA-4 (CD152) monoclonal antibody (Clone: UC10-4F10-11)
Syd Labs provides the following recombinant anti-mouse CTLA-4 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CTLA-4 monoclonal antibody (Clone: 9D9) for flow cytometry
Syd Labs provides the following research grade anti-CTLA-4 antibody biosimilars:
Ipilimumab Biosimilar, research grade, anti-human CTLA-4 monoclonal antibody
Tremelimumab Biosimilar, research grade, anti-human CTLA-4 monoclonal antibody
Recombinant CTLA-4 Proteins:
Biotinylated Human CTLA-4 Protein
Cynomolgus CTLA-4 Protein
Human CTLA-4 Protein
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