Biotinylated Anti-mouse CD8b Antibody (YTS 156.7, Human IgG1 Fc Silent Kappa)

Catalog No.	PA007729.h1Fs-b
Product Name	Biotinylated Recombinant Anti-mouse CD8b Monoclonal Antibody (Clone: YTS 156.7), Human IgG1 Fc Silent Kappa
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Clone	YTS 156.7
Isotype	Human IgG1 Fc Silent Kappa.
Source/Host	The site-directed biotinylated anti-mouse CD8b monoclonal antibody (clone: YTS 156.7) was produced in mammalian cells.
Specificity/Sensitivity	The site-directed biotinylated recombinant YTS 156.7 antibody specifically binds to mouse CD8b protein.
Applications	Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form Of Antibody	0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Conjugate	Biotin-conjugated
Purity	>95% by SDS-PAGE under reducing conditions and HPLC
Shipping	The YTS 156.7 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant rat IgG2b isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007729.h1Fs-b: Biotinylated Recombinant Anti-mouse CD8b Monoclonal Antibody (Clone: YTS 156.7), Human IgG1 Fc Silent Kappa

Recombinant human IgG1 monoclonal antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.

The rat anti-mouse CD8b monoclonal antibody YTS 156.7 (rat IgG2b kappa) reacts with the mouse CD8b protein (T-cell surface glycoprotein CD8 beta chain, Lyt-3 or Ly-3) encoded by the mouse cd8b gene that encodes the CD8b chain of the dimeric CD8 protein. The mouse CD8 protein is primarily responsible for cell-mediated immune defense and T-cell development.
Our recombinant YTS 156.7 antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse CD8b monoclonal antibody (hybridoma clone name or number: YTS 156.7).

References of Anti-Mouse CD8b Monoclonal Antibody (Clone: YTS 156.7):

1. Adding liposomal doxorubicin enhances the abscopal effect induced by radiation/PD1 therapy depending on tumor cell mitochondrial DNA and cGAS/STING

Wang, L., et al. J Immunother Cancer. 2023 Aug;11(8):e006235. PMID: 37640480

“In some experiments, CD8+ T cells were depleted by injecting anti-CD8β (clone YTS 156.7.7) antibodies i.p. 2 days before hRT, on the day of the first hRT, and once weekly thereafter. … Flow-cytometric analyses of tumor-infiltrating lymphocytes (TILs) and single-cell suspensions from tumors, blood, and lymph nodes were performed.”

2. CD8+ T Cells Regulate Monopoiesis and Circulating Ly6C-high Monocyte Levels in Atherosclerosis in Mice

Cochain, C., et al. Circ Res. 2015 Jul 17;117(3):244-53. PMID: 25991812

“Mice were placed on an atherogenic diet for 6 weeks, and treated with weekly intraperitoneal injections of rat antimouse-CD8β (clone YTS-156.7.7) to investigate the regulatory role of CD8+ T cells in monopoiesis and atherosclerosis.”

3. Effects of T-lymphocyte depletion on muscle fibrosis in the mdx mouse

Morrison, J., et al. Am J Pathol. 2005 Jun;166(6):1701-10. PMID: 15920155

“To investigate the role of T lymphocytes in fibrosis, we depleted either CD4 or CD8 or both subsets of T lymphocytes from 4-week-old mdx mice by use of thymectomy followed by specific antibody administration (YTS 156.7.7 and YTS 169.4.7).”

4. Insulin immunization of nonobese diabetic mice induces a protective insulitis characterized by diminished intraislet interferon-gamma transcription

Muir, A., et al. J Clin Invest. 1995 Feb;95(2):628-34. PMID: 7860747

“Synergistic depleting monoclonal antibodies against CD4+ and CD8+ (YTS 156.7) T lymphocytes were used to analyze protective insulitis and intraislet interferon-gamma transcription in nonobese diabetic mice.”

5. Induction of classical transplantation tolerance in the adult

Qin, S. X., et al. J Exp Med. 1989 Mar 1;169(3):779-94. PMID: 2647894

“Of the two CD8 mAbs, YTS 169.4 is anti-Lyt-2 and YTS 156.7 (anti-mouse CD8b antibody) is anti-Lyt3. … The two pairs of synergistic mAbs used in the experiments were all of rat IgG2b isotype.”

For more technical references or data sheets regarding the Recombinant Anti-Mouse CD8b Monoclonal Antibody (Clone: YTS 156.7) formats, please contact our scientific support team at message@sydlabs.com or explore options via Syd Labs.

Syd Labs provides the following in vivo grade recombinant anti-mouse CD8 monoclonal antibodies:
Recombinant Anti-mouse CD8a monoclonal antibody (Clone: 2.43)
Recombinant Anti-mouse CD8a monoclonal antibody (Clone: YTS 169.4)
Recombinant Anti-mouse CD8a monoclonal antibody (Clone: YTS 105.18)
Recombinant Anti-mouse CD8a monoclonal antibody (Clone: 53-6.72)
Recombinant Anti-mouse CD8b monoclonal antibody (Clone: YTS 156.7)
Recombinant Anti-mouse CD8 (Lyt 2.1) monoclonal antibody (Clone: 116-13.1)

Syd Labs provides the following in vivo grade recombinant anti-human CD8 monoclonal antibodies:
Recombinant Anti-human CD8a monoclonal antibody (Clone: OKT8)

Syd Labs provides the following recombinant anti-mouse CD8 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD8a monoclonal antibody (Clone: 2.43) for flow cytometry
Recombinant Anti-mouse CD8a monoclonal antibody (Clone: YTS 169.4) for flow cytometry
Recombinant Anti-mouse CD8a monoclonal antibody (Clone: YTS 105.18) for flow cytometry
Recombinant Anti-mouse CD8b monoclonal antibody (Clone: YTS 156.7) for flow cytometry

Syd Labs provides the following recombinant anti-human CD8 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD8a monoclonal antibody (Clone: OKT8) for flow cytometry