Anti-mouse CTLA-4 Antibody for Flow Cytometry | PA007501.m2b

Catalog No.	PA007501.m2b
Product Name	Recombinant Anti-mouse CTLA-4 Monoclonal Antibody (Clone: 9D9), Mouse IgG2b Kappa
Data Sheet	📄 Download Product Data Sheet (PDF)
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	cytotoxic T-lymphocyte associated antigen 4, cytotoxic T-lymphocyte antigen 4, CD152, CTLA4
Clone	9D9.
Isotype	Mouse IgG2b Kappa.
Source/Host	The anti-mouse CTLA-4 monoclonal antibody (clone: 9D9) for Flow Cytometry was produced in mammalian cells.
Specificity/Sensitivity	The recombinant 9D9 antibody for Flow Cytometry specifically binds to mouse CTLA-4 protein.
Applications	Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form Of Antibody	0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Conjugate	Non-conjugated.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The Recombinant Anti-mouse CTLA-4 Monoclonal Antibody (Clone: 9D9) for Flow Cytometry, Mouse IgG2b Kappa is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
Note	Recombinant mouse IgG2b isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007501.m2b: Recombinant Anti-mouse CTLA-4 Monoclonal Antibody (Clone: 9D9) for Flow Cytometry, Mouse IgG2b Kappa

CTLA-4, a 33-kilodalton immunoglobulin (Ig) superfamily protein, shares significant structural, genomic, and amino acid sequence similarities with CD28. Cytotoxic T-lymphocyte antigen-4 (CTLA-4) is an inhibitory molecule that competes with the stimulatory CD28 for binding to B7 on antigen presenting cells. CTLA-4 and CD28 are both presented on the surface of T-cells. Functioning as a critical immune checkpoint receptor, CTLA-4 plays a central role in downregulating immune responses to maintain homeostasis. It regulates protective immunity development, supports thymocyte activity, and ensures the induction and long-term maintenance of immunological tolerance. These biological properties position CTLA-4 as a dual-action therapeutic target: its agonist applications can suppress excessive immune activity in autoimmune disorders, while antagonist strategies enhance immune activation, particularly in cancer immunotherapy. This dual functionality underscores its clinical relevance in developing treatments for conditions ranging from autoimmune diseases to malignancies, making CTLA-4 a pivotal focus in modern immunotherapy research and precision medicine advancements.

This 9D9 antibody has been reported for use in flow cytometric analysis and blocks the mouse cytotoxic T lymphocyte antigen-4 (CTLA-4). Blocking CTLA-4 removes an inhibitory signal from reducing the activity of T lymphocytes.

References of Anti-Mouse CTLA-4 Monoclonal Antibody (Clone: 9D9)

1. Fc-dependent depletion of tumor-infiltrating regulatory T cells co-defines the efficacy of anti–CTLA-4 therapy against melanoma.
Simpson TR, et al.J Exp Med. 2013 Aug 26;210(9):1695-710. doi: 10.1084/jem.20130091. PMID: 23878181
“The study investigates the mechanism of anti-CTLA-4 therapy using the 9D9 monoclonal antibody in a B16 melanoma mouse model. Tumor-infiltrating lymphocytes were isolated from B16 tumors 7 days after the final dose of anti-CTLA-4 mAb and stained with fluorescently conjugated antibodies against CD4, CD25, CTLA-4 (clone 9D9), FoxP3, CD103, CD8, and CD45.1. Flow cytometry, performed on a modified FACSCanto II cytometer and analyzed with FlowJo software, showed that Tregs were gated as CD4+ FoxP3+ tumor-infiltrating lymphocytes, with the frequency of CTLA-4+ Tregs determined. Anti-CTLA-4 therapy significantly increased CD8+ T cell infiltration in tumors, highlighting the Fc-dependent depletion of regulatory T cells as a key factor in therapeutic efficacy.”

2. Anti-CTLA-4 therapy broadens the melanoma-reactive CD4+ T cell response.
Quezada SA, et al.Oncoimmunology. 2014;3(5):e29032. doi: 10.4161/onci.29032. PMID: 24828551
“This study explores the effects of anti-CTLA-4 therapy using the 9D9 monoclonal antibody on melanoma-reactive CD4+ T cells. Splenocytes were harvested and stained with fluorescently conjugated antibodies against CD4, CD8, CD25, CD44, CTLA-4 (clone 9D9), and PD-1 for flow cytometry analysis. Tetramer staining identified melanoma-specific CD4+ T cells, revealing a 3-fold increase in their frequency following anti-CTLA-4 treatment. Polyfunctional cytokine production was assessed via intracellular staining, demonstrating that anti-CTLA-4 therapy enhances the melanoma-reactive CD4+ T cell response.”

3. CTLA-4 expression on activated T cells by flow cytometry with clone 9D9.
Egen JG, et al.Immunity. 2006 Jun;24(6):803-14. doi: 10.1016/j.immuni.2006.04.009. PMID: 16751384
“This study examines CTLA-4 expression on activated T cells using the 9D9 monoclonal antibody. Activated T cells were stained with PE-conjugated anti-mouse CTLA-4 (clone 9D9), revealing rapid upregulation of CTLA-4 within 24 hours of activation via flow cytometry. CD4+ T cells from OT-II mice were stimulated with OVA peptide, showing CTLA-4 internalization post-stimulation. Blockade with anti-CTLA-4 enhanced T cell expansion, demonstrating the role of CTLA-4 in regulating T cell responses in animal models.”

For more technical references or data sheets regarding the Anti-Mouse CTLA-4 Monoclonal Antibody (Clone: 9D9) formats, please contact our scientific support team at message@sydlabs.com.

Syd Labs provides the following research grade anti-CTLA-4 antibody biosimilars:

Ipilimumab Biosimilar, research grade, anti-human CTLA-4 monoclonal antibody
Tremelimumab Biosimilar, research grade, anti-human CTLA-4 monoclonal antibody

Anti-mouse CTLA-4 Antibody (9D9) for Flow Cytometry from: Recombinant Anti-mouse CTLA-4 Monoclonal Antibody (Clone: 9D9), Mouse IgG2b Kappa: PA007501.m2b Syd Labs